Insights into genome plasticity and pathogenicity of the plant pathogenic bacterium Xanthomonas campestris pv. vesicatoria revealed by the complete genome sequence

Thieme F, Koebnik R, Bekel T, Berger C, Boch J, Buttner D, Caldana C, Gaigalat L, Goesmann A, Kay S, Kirchner O, et al. (2005)
JOURNAL OF BACTERIOLOGY 187(21): 7254-7266.

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The gram-negative plant-pathogenic bacterium Xanthomonas campestris pv. vesicatoria is the causative agent of bacterial spot disease in pepper and tomato plants, which leads to economically important yield losses. This pathosystem has become a well-established model for studying bacterial infection strategies. Here, we present the whole-genome sequence of the pepper-pathogenic Xanthomonas campestris pv. vesicatoria strain 85-10, which comprises a 5.17-Mb circular chromosome and four plasmids. The genome has a high G+C content (64.75%) and signatures of extensive genome plasticity. Whole-genome comparisons revealed a gene order similar to both Xanthomonas axonopodis pv. citri and Xanthomonas campestris pv. campestris and a structure completely different from Xanthomonas oiyzae pv. oryzae. A total of 548 coding sequences (12.2%) are unique to X. campestris pv. vesicatoria. In addition to a type III secretion system, which is essential for pathogenicity, the genome of strain 85-10 encodes all other types of protein secretion systems described so far in gram-negative bacteria. Remarkably, one of the putative type IV secretion systems encoded on the largest plasmid is similar to the Icm/Dot systems of the human pathogens Legionella pneumophild and Coxiella burnetti. Comparisons with other completely sequenced plant pathogens predicted six novel type III effector proteins and several other virulence factors, including adhesins, cell wall-degrading enzymes, and extracellular polysaccharides.
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Thieme F, Koebnik R, Bekel T, et al. Insights into genome plasticity and pathogenicity of the plant pathogenic bacterium Xanthomonas campestris pv. vesicatoria revealed by the complete genome sequence. JOURNAL OF BACTERIOLOGY. 2005;187(21):7254-7266.
Thieme, F., Koebnik, R., Bekel, T., Berger, C., Boch, J., Buttner, D., Caldana, C., et al. (2005). Insights into genome plasticity and pathogenicity of the plant pathogenic bacterium Xanthomonas campestris pv. vesicatoria revealed by the complete genome sequence. JOURNAL OF BACTERIOLOGY, 187(21), 7254-7266.
Thieme, F., Koebnik, R., Bekel, T., Berger, C., Boch, J., Buttner, D., Caldana, C., Gaigalat, L., Goesmann, A., Kay, S., et al. (2005). Insights into genome plasticity and pathogenicity of the plant pathogenic bacterium Xanthomonas campestris pv. vesicatoria revealed by the complete genome sequence. JOURNAL OF BACTERIOLOGY 187, 7254-7266.
Thieme, F., et al., 2005. Insights into genome plasticity and pathogenicity of the plant pathogenic bacterium Xanthomonas campestris pv. vesicatoria revealed by the complete genome sequence. JOURNAL OF BACTERIOLOGY, 187(21), p 7254-7266.
F. Thieme, et al., “Insights into genome plasticity and pathogenicity of the plant pathogenic bacterium Xanthomonas campestris pv. vesicatoria revealed by the complete genome sequence”, JOURNAL OF BACTERIOLOGY, vol. 187, 2005, pp. 7254-7266.
Thieme, F., Koebnik, R., Bekel, T., Berger, C., Boch, J., Buttner, D., Caldana, C., Gaigalat, L., Goesmann, A., Kay, S., Kirchner, O., Lanz, C., Linke, B., McHardy, A.C., Meyer, F., Mittenhuber, G., Nies, D.H., Niesbach-Klosgen, U., Patschkowski, T., Rückert, C., Rupp, O., Schneiker-Bekel, S., Schuster, S.C., Vorhölter, F.-J., Weber, E., Pühler, A., Bonas, U., Bartels, D., Kaiser, O.: Insights into genome plasticity and pathogenicity of the plant pathogenic bacterium Xanthomonas campestris pv. vesicatoria revealed by the complete genome sequence. JOURNAL OF BACTERIOLOGY. 187, 7254-7266 (2005).
Thieme, F, Koebnik, R, Bekel, T, Berger, C, Boch, J, Buttner, D, Caldana, C, Gaigalat, L, Goesmann, Alexander, Kay, S, Kirchner, O, Lanz, C, Linke, B, McHardy, AC, Meyer, F, Mittenhuber, G, Nies, DH, Niesbach-Klosgen, U, Patschkowski, Thomas, Rückert, Christian, Rupp, O, Schneiker-Bekel, Susanne, Schuster, SC, Vorhölter, Frank-Jörg, Weber, E, Pühler, Alfred, Bonas, U, Bartels, D, and Kaiser, O. “Insights into genome plasticity and pathogenicity of the plant pathogenic bacterium Xanthomonas campestris pv. vesicatoria revealed by the complete genome sequence”. JOURNAL OF BACTERIOLOGY 187.21 (2005): 7254-7266.
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Liu H, Dong C, Zhao T, Han J, Wang T, Wen X, Huang Q., PLoS ONE 11(2), 2016
PMID: 26910324
Draft Genome Sequences of Two Xanthomonas euvesicatoria Strains from the Balkan Peninsula.
Vancheva T, Lefeuvre P, Bogatzevska N, Moncheva P, Koebnik R., Genome Announc 3(1), 2015
PMID: 25657284
Draft Genome Sequence of Xanthomonas translucens pv. graminis Pathotype Strain CFBP 2053.
Pesce C, Bolot S, Berthelot E, Bragard C, Cunnac S, Fischer-Le Saux M, Portier P, Arlat M, Gagnevin L, Jacques MA, Noel LD, Carrere S, Koebnik R., Genome Announc 3(5), 2015
PMID: 26450740
Draft Genome Sequences of Two Xanthomonas vesicatoria Strains from the Balkan Peninsula.
Vancheva T, Bogatzevska N, Moncheva P, Lefeuvre P, Koebnik R., Genome Announc 3(1), 2015
PMID: 25676765
Comparative genomics of 43 strains of Xanthomonas citri pv. citri reveals the evolutionary events giving rise to pathotypes with different host ranges.
Gordon JL, Lefeuvre P, Escalon A, Barbe V, Cruveiller S, Gagnevin L, Pruvost O., BMC Genomics 16(), 2015
PMID: 26699528
Refined requirements for protein regions important for activity of the TALE AvrBs3.
Schreiber T, Sorgatz A, List F, Bluher D, Thieme S, Wilmanns M, Bonas U., PLoS ONE 10(3), 2015
PMID: 25781334
Comparative Genomics of Pathogenic and Nonpathogenic Strains of Xanthomonas arboricola Unveil Molecular and Evolutionary Events Linked to Pathoadaptation.
Cesbron S, Briand M, Essakhi S, Gironde S, Boureau T, Manceau C, Fischer-Le Saux M, Jacques MA., Front Plant Sci 6(), 2015
PMID: 26734033
Complete sequence and detailed analysis of the first indigenous plasmid from Xanthomonas oryzae pv. oryzicola.
Niu XN, Wei ZQ, Zou HF, Xie GG, Wu F, Li KJ, Jiang W, Tang JL, He YQ., BMC Microbiol. 15(), 2015
PMID: 26498126
Development of a Species-specific PCR Assay for Three Xanthomonas Species, Causing Bulb and Flower Diseases, Based on Their Genome Sequences.
Back CG, Lee SY, Lee BJ, Yea MC, Kim SM, Kang IK, Cha JS, Jung HY., Plant Pathol. J. 31(3), 2015
PMID: 26361469
Ecological genomics in Xanthomonas: the nature of genetic adaptation with homologous recombination and host shifts.
Huang CL, Pu PH, Huang HJ, Sung HM, Liaw HJ, Chen YM, Chen CM, Huang MB, Osada N, Gojobori T, Pai TW, Chen YT, Hwang CC, Chiang TY., BMC Genomics 16(), 2015
PMID: 25879893
Draft genome sequence of Xanthomonas axonopodis pathovar vasculorum NCPPB 900.
Harrison J, Studholme DJ., FEMS Microbiol. Lett. 360(2), 2014
PMID: 25263632
HrcT is a key component of the type III secretion system in Xanthomonas spp. and also regulates the expression of the key hrp transcriptional activator HrpX.
Liu ZY, Zou LF, Xue XB, Cai LL, Ma WX, Xiong L, Ji ZY, Chen GY., Appl. Environ. Microbiol. 80(13), 2014
PMID: 24747909
Small non-coding RNAs in plant-pathogenic Xanthomonas spp.
Abendroth U, Schmidtke C, Bonas U., RNA Biol 11(5), 2014
PMID: 24667380
Distribution and biological role of the oligopeptide-binding protein (OppA) in Xanthomonas species.
Oshiro EE, Tavares MB, Suzuki CF, Pimenta DC, Angeli CB, de Oliveira JC, Ferro MI, Ferreira LC, Ferreira RC., Genet. Mol. Biol. 33(2), 2010
PMID: 21637492
Isolation of Pseudomonas spp. from Diseased Capsicum chinense (Habanero Pepper) Plants in Yucatan, Mexico
Moguel-S.alazar F, Quijano-R.amayo A, Keb-L.lanes M, Moreno-V.alenzuela O, Islas-F.lores I., 2007
PMID: IND43933718

112 References

Data provided by Europe PubMed Central.

Synopsis on the taxonomy of the genus xanthomonas.
Vauterin L, Rademaker J, Swings J., Phytopathology 90(7), 2000
PMID: 18944485
The roles of plasmids in phytopathogenic bacteria: mobile arsenals?
Vivian A, Murillo J, Jackson RW., Microbiology (Reading, Engl.) 147(Pt 4), 2001
PMID: 11283273
Quorum sensing in plant-pathogenic bacteria.
Von Bodman SB, Bauer WD, Coplin DL., Annu Rev Phytopathol 41(), 2003
PMID: 12730390
A bacterial cell-cell communication signal with cross-kingdom structural analogues.
Wang LH, He Y, Gao Y, Wu JE, Dong YH, He C, Wang SX, Weng LX, Xu JL, Tay L, Fang RX, Zhang LH., Mol. Microbiol. 51(3), 2004
PMID: 14731288
HrpG, a key hrp regulatory protein of Xanthomonas campestris pv. vesicatoria is homologous to two-component response regulators.
Wengelnik K, Van den Ackerveken G, Bonas U., Mol. Plant Microbe Interact. 9(8), 1996
PMID: 8870269
Avirulence gene avrRxv from Xanthomonas campestris pv. vesicatoria specifies resistance on tomato line Hawaii 7998.
Whalen MC, Wang JF, Carland FM, Heiskell ME, Dahlbeck D, Minsavage GV, Jones JB, Scott JW, Stall RE, Staskawicz BJ., Mol. Plant Microbe Interact. 6(5), 1993
PMID: 8274773

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