A rapid reporter system using GFP as a reporter protein for identification and screening of synthetic stationary-phase promoters in Escherichia coli

Miksch G, Bettenworth F, Friehs K, Flaschel E, Saalbach A, Nattkemper TW (2006)
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY 70(2): 229-236.

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Abstract
To develop a rapid reporter system for the screening of stationary-phase promoters in Escherichia coli, the expression pattern of the green fluorescent protein (GFP) during bacterial cultivation was compared with that of the commonly used beta-galactosidase. Using GFP with enhanced fluorescence, the expression pattern of both reporter systems GFP and beta-galactosidase were similar and showed a typical induction of gene activity of the reporter genes, i.e. increase of expression at the transition from exponential to stationary phase. The expression was affected by the culture medium, i.e. in contrast to the complex medium (LB medium), the stationary-phase specific induction was only observed in synthetic medium (M9) when amino acids were added, whereas there was generally no induction in MOPS medium. To develop a rapid screening method on agar plates for stationary-phase promoters, a photographic approach was used, continued with computational image treatment. A screening method is presented which enables an on-line monitoring of gene activity.
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Miksch G, Bettenworth F, Friehs K, Flaschel E, Saalbach A, Nattkemper TW. A rapid reporter system using GFP as a reporter protein for identification and screening of synthetic stationary-phase promoters in Escherichia coli. APPLIED MICROBIOLOGY AND BIOTECHNOLOGY. 2006;70(2):229-236.
Miksch, G., Bettenworth, F., Friehs, K., Flaschel, E., Saalbach, A., & Nattkemper, T. W. (2006). A rapid reporter system using GFP as a reporter protein for identification and screening of synthetic stationary-phase promoters in Escherichia coli. APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, 70(2), 229-236.
Miksch, G., Bettenworth, F., Friehs, K., Flaschel, E., Saalbach, A., and Nattkemper, T. W. (2006). A rapid reporter system using GFP as a reporter protein for identification and screening of synthetic stationary-phase promoters in Escherichia coli. APPLIED MICROBIOLOGY AND BIOTECHNOLOGY 70, 229-236.
Miksch, G., et al., 2006. A rapid reporter system using GFP as a reporter protein for identification and screening of synthetic stationary-phase promoters in Escherichia coli. APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, 70(2), p 229-236.
G. Miksch, et al., “A rapid reporter system using GFP as a reporter protein for identification and screening of synthetic stationary-phase promoters in Escherichia coli”, APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, vol. 70, 2006, pp. 229-236.
Miksch, G., Bettenworth, F., Friehs, K., Flaschel, E., Saalbach, A., Nattkemper, T.W.: A rapid reporter system using GFP as a reporter protein for identification and screening of synthetic stationary-phase promoters in Escherichia coli. APPLIED MICROBIOLOGY AND BIOTECHNOLOGY. 70, 229-236 (2006).
Miksch, Gerd, Bettenworth, F, Friehs, Karl, Flaschel, Erwin, Saalbach, A, and Nattkemper, Tim Wilhelm. “A rapid reporter system using GFP as a reporter protein for identification and screening of synthetic stationary-phase promoters in Escherichia coli”. APPLIED MICROBIOLOGY AND BIOTECHNOLOGY 70.2 (2006): 229-236.
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2 Citations in Europe PMC

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58 References

Data provided by Europe PubMed Central.

A novel phosphate-regulated expression vector in Escherichia coli.
Su TZ, Schweizer H, Oxender DL., Gene 90(1), 1990
PMID: 2379833

WW, 1998
Identification and characterization of stationary phase-inducible genes in Escherichia coli.
Weichart D, Lange R, Henneberg N, Hengge-Aronis R., Mol. Microbiol. 10(2), 1993
PMID: 7934831
Sequences in the -35 region of Escherichia coli rpoS-dependent genes promote transcription by E sigma S.
Wise A, Brems R, Ramakrishnan V, Villarejo M., J. Bacteriol. 178(10), 1996
PMID: 8631665
Regulation of the Escherichia coli rmf gene encoding the ribosome modulation factor: growth phase- and growth rate-dependent control.
Yamagishi M, Matsushima H, Wada A, Sakagami M, Fujita N, Ishihama A., EMBO J. 12(2), 1993
PMID: 8440252
Molecular characterization of the promoter of osmY, an rpoS-dependent gene.
Yim HH, Brems RL, Villarejo M., J. Bacteriol. 176(1), 1994
PMID: 8282684

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