Dynamics of amino acid metabolism of primary human liver cells in 3D bioreactors

Guthke R, Zeilinger K, Sickinger S, Schmidt-Heck W, Büntemeyer H, Iding K, Lehmann J, Pfaff M, Pless G, Gerlach JC (2006)
BIOPROCESS AND BIOSYSTEMS ENGINEERING 28(5): 331-340.

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The kinetics of 18 amino acids, ammonia (NH3) and urea (UREA) in 18 liver cell bioreactor runs were analyzed and simulated by a two-compartment model consisting of a system of 42 differential equations. The model parameters, most of them representing enzymatic activities, were identified and their values discussed with respect to the different liver cell bioreactor performance levels. The nitrogen balance based model was used as a tool to quantify the variability of runs and to describe different kinetic patterns of the amino acid metabolism, in particular with respect to glutamate (GLU) and aspartate (ASP).
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Guthke R, Zeilinger K, Sickinger S, et al. Dynamics of amino acid metabolism of primary human liver cells in 3D bioreactors. BIOPROCESS AND BIOSYSTEMS ENGINEERING. 2006;28(5):331-340.
Guthke, R., Zeilinger, K., Sickinger, S., Schmidt-Heck, W., Büntemeyer, H., Iding, K., Lehmann, J., et al. (2006). Dynamics of amino acid metabolism of primary human liver cells in 3D bioreactors. BIOPROCESS AND BIOSYSTEMS ENGINEERING, 28(5), 331-340.
Guthke, R., Zeilinger, K., Sickinger, S., Schmidt-Heck, W., Büntemeyer, H., Iding, K., Lehmann, J., Pfaff, M., Pless, G., and Gerlach, J. C. (2006). Dynamics of amino acid metabolism of primary human liver cells in 3D bioreactors. BIOPROCESS AND BIOSYSTEMS ENGINEERING 28, 331-340.
Guthke, R., et al., 2006. Dynamics of amino acid metabolism of primary human liver cells in 3D bioreactors. BIOPROCESS AND BIOSYSTEMS ENGINEERING, 28(5), p 331-340.
R. Guthke, et al., “Dynamics of amino acid metabolism of primary human liver cells in 3D bioreactors”, BIOPROCESS AND BIOSYSTEMS ENGINEERING, vol. 28, 2006, pp. 331-340.
Guthke, R., Zeilinger, K., Sickinger, S., Schmidt-Heck, W., Büntemeyer, H., Iding, K., Lehmann, J., Pfaff, M., Pless, G., Gerlach, J.C.: Dynamics of amino acid metabolism of primary human liver cells in 3D bioreactors. BIOPROCESS AND BIOSYSTEMS ENGINEERING. 28, 331-340 (2006).
Guthke, R, Zeilinger, K, Sickinger, S, Schmidt-Heck, W, Büntemeyer, Heino, Iding, K, Lehmann, J, Pfaff, M, Pless, G, and Gerlach, JC. “Dynamics of amino acid metabolism of primary human liver cells in 3D bioreactors”. BIOPROCESS AND BIOSYSTEMS ENGINEERING 28.5 (2006): 331-340.
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