The surface (S)-layer gene cspB of Corynebacterium glutamicum is transcriptionally activated by a LuxR-type regulator and located on a 6 kb genomic island absent from the type strain ATCC 13032

Hansmeier N, Albersmeier A, Tauch A, Damberg T, Ros R, Anselmetti D, Pühler A, Kalinowski J (2006)
Microbiology 152(4): 923-935.

Download
Es wurde kein Volltext hochgeladen. Nur Publikationsnachweis!
Zeitschriftenaufsatz | Veröffentlicht | Englisch
Abstract / Bemerkung
The surface (S)-layer gene region of the Gram-positive bacterium Corynebacterium glutamicum ATCC 14067 was identified on fosmid clones, sequenced and compared with the genome sequence of C. glutamicum ATCC 13032, whose cell surface is devoid of an ordered S-layer lattice. A 5-97 kb DNA region that is absent from the C. glutamicum ATCC 13032 chromosome was identified. This region includes cspB, the structural gene encoding the S-layer protomer PS2, and six additional coding sequences. PCR experiments demonstrated that the respective DNA region is conserved in different C. glutamicum wild-type strains capable of S-layer formation. The DNA region is flanked by a 7 bp direct repeat, suggesting that illegitimate recombination might be responsible for gene loss in C. glutamicum ATCC 13032. Transfer of the cloned cspB gene restored the PS2(-) phenotype of C. glutamicum ATCC 13032, as confirmed by visualization of the PS2 proteins by SDS-PAGE and imaging of ordered hexagonal S-layer lattices on living C. glutamicum cells by atomic force microscopy. Furthermore, the promoter of the cspB gene was mapped by 5 ' rapid amplification of cDNA ends PCR and the corresponding DNA fragment was used in DNA affinity purification assays. A 30 kDa protein specifically binding to the promoter region of the cspB gene was purified. Matrix-assisted laser desorption ionization time-of-flight mass spectrometry and peptide mass fingerprinting of the purified protein led to the identification of the putative transcriptional regulator Cg2831, belonging to the LuxR regulatory protein family. Disruption of the cg2831 gene in C. glutamicum resulted in an almost complete loss of PS2 synthesis. These results suggested that Cg2831 is a transcriptional activator of cspB gene expression in C. glutamicum.
Erscheinungsjahr
Zeitschriftentitel
Microbiology
Band
152
Zeitschriftennummer
4
Seite
923-935
ISSN
eISSN
PUB-ID

Zitieren

Hansmeier N, Albersmeier A, Tauch A, et al. The surface (S)-layer gene cspB of Corynebacterium glutamicum is transcriptionally activated by a LuxR-type regulator and located on a 6 kb genomic island absent from the type strain ATCC 13032. Microbiology. 2006;152(4):923-935.
Hansmeier, N., Albersmeier, A., Tauch, A., Damberg, T., Ros, R., Anselmetti, D., Pühler, A., et al. (2006). The surface (S)-layer gene cspB of Corynebacterium glutamicum is transcriptionally activated by a LuxR-type regulator and located on a 6 kb genomic island absent from the type strain ATCC 13032. Microbiology, 152(4), 923-935. doi:10.1099/mic.0.28673-0
Hansmeier, N., Albersmeier, A., Tauch, A., Damberg, T., Ros, R., Anselmetti, D., Pühler, A., and Kalinowski, J. (2006). The surface (S)-layer gene cspB of Corynebacterium glutamicum is transcriptionally activated by a LuxR-type regulator and located on a 6 kb genomic island absent from the type strain ATCC 13032. Microbiology 152, 923-935.
Hansmeier, N., et al., 2006. The surface (S)-layer gene cspB of Corynebacterium glutamicum is transcriptionally activated by a LuxR-type regulator and located on a 6 kb genomic island absent from the type strain ATCC 13032. Microbiology, 152(4), p 923-935.
N. Hansmeier, et al., “The surface (S)-layer gene cspB of Corynebacterium glutamicum is transcriptionally activated by a LuxR-type regulator and located on a 6 kb genomic island absent from the type strain ATCC 13032”, Microbiology, vol. 152, 2006, pp. 923-935.
Hansmeier, N., Albersmeier, A., Tauch, A., Damberg, T., Ros, R., Anselmetti, D., Pühler, A., Kalinowski, J.: The surface (S)-layer gene cspB of Corynebacterium glutamicum is transcriptionally activated by a LuxR-type regulator and located on a 6 kb genomic island absent from the type strain ATCC 13032. Microbiology. 152, 923-935 (2006).
Hansmeier, N, Albersmeier, Andreas, Tauch, Andreas, Damberg, T, Ros, R, Anselmetti, Dario, Pühler, Alfred, and Kalinowski, Jörn. “The surface (S)-layer gene cspB of Corynebacterium glutamicum is transcriptionally activated by a LuxR-type regulator and located on a 6 kb genomic island absent from the type strain ATCC 13032”. Microbiology 152.4 (2006): 923-935.

28 Zitationen in Europe PMC

Daten bereitgestellt von Europe PubMed Central.

The RamA regulon: complex regulatory interactions in relation to central metabolism in Corynebacterium glutamicum.
Shah A, Blombach B, Gauttam R, Eikmanns BJ., Appl Microbiol Biotechnol 102(14), 2018
PMID: 29804137
Tetramethylpyrazine-Inducible Promoter Region from Rhodococcus jostii TMP1.
Stanislauskienė R, Kutanovas S, Kalinienė L, Bratchikov M, Meškys R., Molecules 23(7), 2018
PMID: 29941849
Isolation and Transcriptome Analysis of Phenol-Degrading Bacterium From Carbon-Sand Filters in a Full-Scale Drinking Water Treatment Plant.
Gu Q, Wu Q, Zhang J, Guo W, Ding Y, Wang J, Wu H, Sun M, Hou L, Wei X, Zhang Y., Front Microbiol 9(), 2018
PMID: 30298058
Regulons of global transcription factors in Corynebacterium glutamicum.
Toyoda K, Inui M., Appl Microbiol Biotechnol 100(1), 2016
PMID: 26496920
Corynebacterium glutamicum possesses β-N-acetylglucosaminidase.
Matano C, Kolkenbrock S, Hamer SN, Sgobba E, Moerschbacher BM, Wendisch VF., BMC Microbiol 16(1), 2016
PMID: 27492186
Proteomes of host cell membranes modified by intracellular activities of Salmonella enterica.
Vorwerk S, Krieger V, Deiwick J, Hensel M, Hansmeier N., Mol Cell Proteomics 14(1), 2015
PMID: 25348832
Direct L-lysine production from cellobiose by Corynebacterium glutamicum displaying beta-glucosidase on its cell surface.
Adachi N, Takahashi C, Ono-Murota N, Yamaguchi R, Tanaka T, Kondo A., Appl Microbiol Biotechnol 97(16), 2013
PMID: 23749228
Corynebacterium diphtheriae invasion-associated protein (DIP1281) is involved in cell surface organization, adhesion and internalization in epithelial cells.
Ott L, Höller M, Gerlach RG, Hensel M, Rheinlaender J, Schäffer TE, Burkovski A., BMC Microbiol 10(), 2010
PMID: 20051108
The transcriptional regulators RamA and RamB are involved in the regulation of glycogen synthesis in Corynebacterium glutamicum.
Seibold GM, Hagmann CT, Schietzel M, Emer D, Auchter M, Schreiner J, Eikmanns BJ., Microbiology 156(pt 4), 2010
PMID: 20056699
The regulator RamA influences cmytA transcription and cell morphology of Corynebacterium ammoniagenes.
Lee SM, Lee JY, Park KJ, Park JS, Ha UH, Kim Y, Lee HS., Curr Microbiol 61(2), 2010
PMID: 20107993
Characterization and separate activities of the two promoters of the Lactobacillus brevis S-layer protein gene.
Hynönen U, Avall-Jääskeläinen S, Palva A., Appl Microbiol Biotechnol 87(2), 2010
PMID: 20229202
A deficiency in arabinogalactan biosynthesis affects Corynebacterium glutamicum mycolate outer membrane stability.
Bou Raad R, Méniche X, de Sousa-d'Auria C, Chami M, Salmeron C, Tropis M, Labarre C, Daffé M, Houssin C, Bayan N., J Bacteriol 192(11), 2010
PMID: 20363942
Cell growth and cell division in the rod-shaped actinomycete Corynebacterium glutamicum.
Letek M, Fiuza M, Ordóñez E, Villadangos AF, Ramos A, Mateos LM, Gil JA., Antonie Van Leeuwenhoek 94(1), 2008
PMID: 18283557
Analysis of subcellular surface structure, function and dynamics.
Anselmetti D, Hansmeier N, Kalinowski J, Martini J, Merkle T, Palmisano R, Ros R, Schmied K, Sischka A, Toensing K., Anal Bioanal Chem 387(1), 2007
PMID: 17082883

Export

Markieren/ Markierung löschen
Markierte Publikationen

Open Data PUB

Web of Science

Dieser Datensatz im Web of Science®

Quellen

PMID: 16549657
PubMed | Europe PMC

Suchen in

Google Scholar