Integrin alpha(5)beta(1): a new purification strategy based on immobilized peptides

Wobbe L, Zimmermann D, Wissbock M, Urman S, Sewald K, Malesevic M, Sewald N (2005)
JOURNAL OF PEPTIDE RESEARCH 66: 22-29.

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Abstract
Novel efficient and robust affinity chromatography material: There are several strategies known for the purification of integrins by affinity chromatography, but the disadvantages of common strategies like insufficient selectivity or compelling conditions for the elution still require alternatives. A new strategy, based on the immobilized C-terminally modified peptide Ac-Gly-Ala-c-(Cys(SS)-Arg-Arg-Glu-Thr-Ala-Trp-Ala-Cys(SS))-Gly-Ala-O(CH2 CH2O)(2)CH2CH2-NH2 allows for the affinity purification of the integrin alpha(5)beta(1). While RGD peptides have been proven in the past to be inappropriate for selective purification of integrins by affinity chromatography, the new peptide can be efficiently used for selective enrichment of the integrin alpha(5)beta(1). It is a specific ligand of the target protein, but does not contain an RGD sequence. The application of well-characterized affinity chromatography material with a site-specifically immobilized peptide allows to obtain integrin alpha(5)beta(1) in a single chromatography step without contamination by other integrins. This process combines the advantages of a selective and monospecific protein-ligand recognition with mild elution conditions and a low sensitivity of the immobilized ligand with respect to column regeneration.
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Wobbe L, Zimmermann D, Wissbock M, et al. Integrin alpha(5)beta(1): a new purification strategy based on immobilized peptides. JOURNAL OF PEPTIDE RESEARCH. 2005;66:22-29.
Wobbe, L., Zimmermann, D., Wissbock, M., Urman, S., Sewald, K., Malesevic, M., & Sewald, N. (2005). Integrin alpha(5)beta(1): a new purification strategy based on immobilized peptides. JOURNAL OF PEPTIDE RESEARCH, 66, 22-29.
Wobbe, L., Zimmermann, D., Wissbock, M., Urman, S., Sewald, K., Malesevic, M., and Sewald, N. (2005). Integrin alpha(5)beta(1): a new purification strategy based on immobilized peptides. JOURNAL OF PEPTIDE RESEARCH 66, 22-29.
Wobbe, L., et al., 2005. Integrin alpha(5)beta(1): a new purification strategy based on immobilized peptides. JOURNAL OF PEPTIDE RESEARCH, 66, p 22-29.
L. Wobbe, et al., “Integrin alpha(5)beta(1): a new purification strategy based on immobilized peptides”, JOURNAL OF PEPTIDE RESEARCH, vol. 66, 2005, pp. 22-29.
Wobbe, L., Zimmermann, D., Wissbock, M., Urman, S., Sewald, K., Malesevic, M., Sewald, N.: Integrin alpha(5)beta(1): a new purification strategy based on immobilized peptides. JOURNAL OF PEPTIDE RESEARCH. 66, 22-29 (2005).
Wobbe, Lutz, Zimmermann, D, Wissbock, M, Urman, S, Sewald, K, Malesevic, M, and Sewald, Norbert. “Integrin alpha(5)beta(1): a new purification strategy based on immobilized peptides”. JOURNAL OF PEPTIDE RESEARCH 66 (2005): 22-29.
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PMID: 16650057
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