Utilization of soluble starch by a recombinant Corynebacterium glutamicum strain: Growth and lysine production

Seibold G, Auchter M, Berens S, Kalinowski J, Eikmanns BJ (2006)
JOURNAL OF BIOTECHNOLOGY 124(2): 381-391.

Journal Article | Published | English

No fulltext has been uploaded

Author
; ; ; ;
Abstract
Corynebacterium glutamicum, well known for the industrial production of amino acids, grows aerobically on a variety of mono- and disaccharides and on alcohols and organic acids as single or combined sources of carbon and energy. Members of the genera Corynebacterium and Brevibacterium were here tested for their ability to use the homopolysaccharide starch as a substrate for growth. None of the 24 type strains tested showed growth on or degradation of this substrate, indicating that none of the strains synthesized and secreted starch-degrading enzymes. Introducing the Streptomyces griseus amy gene on an expression vector into the lysine-producer C. glutamicum DM1730, we constructed a C. glutamicum strain synthesizing and secreting a-amylase into the culture broth. Although some high-molecular-weight degradation products remained in the culture broth, this recombinant strain effectively used soluble starch as carbon and energy substrate for growth and also for lysine production. Thus, employment of our construct allows avoidance of the cost-intensive enzymatic hydrolysis of the starch, which commercially is used as a substrate in industrial amino acid fermentations. (c) 2006 Elsevier B.V. All rights reserved.
Publishing Year
ISSN
PUB-ID

Cite this

Seibold G, Auchter M, Berens S, Kalinowski J, Eikmanns BJ. Utilization of soluble starch by a recombinant Corynebacterium glutamicum strain: Growth and lysine production. JOURNAL OF BIOTECHNOLOGY. 2006;124(2):381-391.
Seibold, G., Auchter, M., Berens, S., Kalinowski, J., & Eikmanns, B. J. (2006). Utilization of soluble starch by a recombinant Corynebacterium glutamicum strain: Growth and lysine production. JOURNAL OF BIOTECHNOLOGY, 124(2), 381-391.
Seibold, G., Auchter, M., Berens, S., Kalinowski, J., and Eikmanns, B. J. (2006). Utilization of soluble starch by a recombinant Corynebacterium glutamicum strain: Growth and lysine production. JOURNAL OF BIOTECHNOLOGY 124, 381-391.
Seibold, G., et al., 2006. Utilization of soluble starch by a recombinant Corynebacterium glutamicum strain: Growth and lysine production. JOURNAL OF BIOTECHNOLOGY, 124(2), p 381-391.
G. Seibold, et al., “Utilization of soluble starch by a recombinant Corynebacterium glutamicum strain: Growth and lysine production”, JOURNAL OF BIOTECHNOLOGY, vol. 124, 2006, pp. 381-391.
Seibold, G., Auchter, M., Berens, S., Kalinowski, J., Eikmanns, B.J.: Utilization of soluble starch by a recombinant Corynebacterium glutamicum strain: Growth and lysine production. JOURNAL OF BIOTECHNOLOGY. 124, 381-391 (2006).
Seibold, Gerd, Auchter, Marc, Berens, Stephan, Kalinowski, Jörn, and Eikmanns, Bernhard J. “Utilization of soluble starch by a recombinant Corynebacterium glutamicum strain: Growth and lysine production”. JOURNAL OF BIOTECHNOLOGY 124.2 (2006): 381-391.
This data publication is cited in the following publications:
This publication cites the following data publications:

36 Citations in Europe PMC

Data provided by Europe PubMed Central.

Synthesis of chemicals by metabolic engineering of microbes.
Sun X, Shen X, Jain R, Lin Y, Wang J, Sun J, Wang J, Yan Y, Yuan Q., Chem Soc Rev 44(11), 2015
PMID: 25940754
Engineering of Corynebacterium glutamicum for growth and L-lysine and lycopene production from N-acetyl-glucosamine.
Matano C, Uhde A, Youn JW, Maeda T, Clermont L, Marin K, Kramer R, Wendisch VF, Seibold GM., Appl. Microbiol. Biotechnol. 98(12), 2014
PMID: 24668244
Engineering biotin prototrophic Corynebacterium glutamicum strains for amino acid, diamine and carotenoid production.
Peters-Wendisch P, Gotker S, Heider SA, Komati Reddy G, Nguyen AQ, Stansen KC, Wendisch VF., J. Biotechnol. 192 Pt B(), 2014
PMID: 24486440
Maltose uptake by the novel ABC transport system MusEFGK2I causes increased expression of ptsG in Corynebacterium glutamicum.
Henrich A, Kuhlmann N, Eck AW, Kramer R, Seibold GM., J. Bacteriol. 195(11), 2013
PMID: 23543710

52 References

Data provided by Europe PubMed Central.

Targeted disruption of the alpha-amylase gene in the hyperthermophilic archaeon Sulfolobus solfataricus.
Worthington P, Hoang V, Perez-Pomares F, Blum P., J. Bacteriol. 185(2), 2003
PMID: 12511494

Export

0 Marked Publications

Open Data PUB

Web of Science

View record in Web of Science®

Sources

PMID: 16488498
PubMed | Europe PMC

Search this title in

Google Scholar