Utilization of soluble starch by a recombinant Corynebacterium glutamicum strain: Growth and lysine production

Seibold G, Auchter M, Berens S, Kalinowski J, Eikmanns BJ (2006)
JOURNAL OF BIOTECHNOLOGY 124(2): 381-391.

Download
No fulltext has been uploaded. References only!
Journal Article | Original Article | Published | English

No fulltext has been uploaded

Author
; ; ; ;
Abstract
Corynebacterium glutamicum, well known for the industrial production of amino acids, grows aerobically on a variety of mono- and disaccharides and on alcohols and organic acids as single or combined sources of carbon and energy. Members of the genera Corynebacterium and Brevibacterium were here tested for their ability to use the homopolysaccharide starch as a substrate for growth. None of the 24 type strains tested showed growth on or degradation of this substrate, indicating that none of the strains synthesized and secreted starch-degrading enzymes. Introducing the Streptomyces griseus amy gene on an expression vector into the lysine-producer C. glutamicum DM1730, we constructed a C. glutamicum strain synthesizing and secreting a-amylase into the culture broth. Although some high-molecular-weight degradation products remained in the culture broth, this recombinant strain effectively used soluble starch as carbon and energy substrate for growth and also for lysine production. Thus, employment of our construct allows avoidance of the cost-intensive enzymatic hydrolysis of the starch, which commercially is used as a substrate in industrial amino acid fermentations. (c) 2006 Elsevier B.V. All rights reserved.
Publishing Year
ISSN
PUB-ID

Cite this

Seibold G, Auchter M, Berens S, Kalinowski J, Eikmanns BJ. Utilization of soluble starch by a recombinant Corynebacterium glutamicum strain: Growth and lysine production. JOURNAL OF BIOTECHNOLOGY. 2006;124(2):381-391.
Seibold, G., Auchter, M., Berens, S., Kalinowski, J., & Eikmanns, B. J. (2006). Utilization of soluble starch by a recombinant Corynebacterium glutamicum strain: Growth and lysine production. JOURNAL OF BIOTECHNOLOGY, 124(2), 381-391. doi:10.1016/j.jbiotec.2005.12.027
Seibold, G., Auchter, M., Berens, S., Kalinowski, J., and Eikmanns, B. J. (2006). Utilization of soluble starch by a recombinant Corynebacterium glutamicum strain: Growth and lysine production. JOURNAL OF BIOTECHNOLOGY 124, 381-391.
Seibold, G., et al., 2006. Utilization of soluble starch by a recombinant Corynebacterium glutamicum strain: Growth and lysine production. JOURNAL OF BIOTECHNOLOGY, 124(2), p 381-391.
G. Seibold, et al., “Utilization of soluble starch by a recombinant Corynebacterium glutamicum strain: Growth and lysine production”, JOURNAL OF BIOTECHNOLOGY, vol. 124, 2006, pp. 381-391.
Seibold, G., Auchter, M., Berens, S., Kalinowski, J., Eikmanns, B.J.: Utilization of soluble starch by a recombinant Corynebacterium glutamicum strain: Growth and lysine production. JOURNAL OF BIOTECHNOLOGY. 124, 381-391 (2006).
Seibold, Gerd, Auchter, Marc, Berens, Stephan, Kalinowski, Jörn, and Eikmanns, Bernhard J. “Utilization of soluble starch by a recombinant Corynebacterium glutamicum strain: Growth and lysine production”. JOURNAL OF BIOTECHNOLOGY 124.2 (2006): 381-391.
This data publication is cited in the following publications:
This publication cites the following data publications:

50 Citations in Europe PMC

Data provided by Europe PubMed Central.

Production and glucosylation of C50 and C 40 carotenoids by metabolically engineered Corynebacterium glutamicum.
Heider SA, Peters-Wendisch P, Netzer R, Stafnes M, Brautaset T, Wendisch VF., Appl Microbiol Biotechnol 98(3), 2014
PMID: 24270893
Engineering biotin prototrophic Corynebacterium glutamicum strains for amino acid, diamine and carotenoid production.
Peters-Wendisch P, Götker S, Heider SA, Komati Reddy G, Nguyen AQ, Stansen KC, Wendisch VF., J Biotechnol 192 Pt B(), 2014
PMID: 24486440
Engineering of Corynebacterium glutamicum for growth and L-lysine and lycopene production from N-acetyl-glucosamine.
Matano C, Uhde A, Youn JW, Maeda T, Clermont L, Marin K, Krämer R, Wendisch VF, Seibold GM., Appl Microbiol Biotechnol 98(12), 2014
PMID: 24668244
Metabolic engineering for the microbial production of carotenoids and related products with a focus on the rare C50 carotenoids.
Heider SA, Peters-Wendisch P, Wendisch VF, Beekwilder J, Brautaset T., Appl Microbiol Biotechnol 98(10), 2014
PMID: 24687754
Production of L-lysine on different silage juices using genetically engineered Corynebacterium glutamicum.
Neuner A, Wagner I, Sieker T, Ulber R, Schneider K, Peifer S, Heinzle E., J Biotechnol 163(2), 2013
PMID: 22898177
Accelerated pentose utilization by Corynebacterium glutamicum for accelerated production of lysine, glutamate, ornithine and putrescine.
Meiswinkel TM, Gopinath V, Lindner SN, Nampoothiri KM, Wendisch VF., Microb Biotechnol 6(2), 2013
PMID: 23164409
Bio-based production of organic acids with Corynebacterium glutamicum.
Wieschalka S, Blombach B, Bott M, Eikmanns BJ., Microb Biotechnol 6(2), 2013
PMID: 23199277
Crude glycerol-based production of amino acids and putrescine by Corynebacterium glutamicum.
Meiswinkel TM, Rittmann D, Lindner SN, Wendisch VF., Bioresour Technol 145(), 2013
PMID: 23562176
Maltose uptake by the novel ABC transport system MusEFGK2I causes increased expression of ptsG in Corynebacterium glutamicum.
Henrich A, Kuhlmann N, Eck AW, Krämer R, Seibold GM., J Bacteriol 195(11), 2013
PMID: 23543710
Metabolic engineering of Corynebacterium glutamicum aimed at alternative carbon sources and new products.
Zahoor A, Lindner SN, Wendisch VF., Comput Struct Biotechnol J 3(), 2012
PMID: 24688664
Tools for genetic manipulations in Corynebacterium glutamicum and their applications.
Nešvera J, Pátek M., Appl Microbiol Biotechnol 90(5), 2011
PMID: 21519933
Amino acid production from rice straw and wheat bran hydrolysates by recombinant pentose-utilizing Corynebacterium glutamicum.
Gopinath V, Meiswinkel TM, Wendisch VF, Nampoothiri KM., Appl Microbiol Biotechnol 92(5), 2011
PMID: 21796382
Increased glucose utilization in Corynebacterium glutamicum by use of maltose, and its application for the improvement of L-valine productivity.
Krause FS, Henrich A, Blombach B, Krämer R, Eikmanns BJ, Seibold GM., Appl Environ Microbiol 76(1), 2010
PMID: 19880641
Putrescine production by engineered Corynebacterium glutamicum.
Schneider J, Wendisch VF., Appl Microbiol Biotechnol 88(4), 2010
PMID: 20661733
Link between phosphate starvation and glycogen metabolism in Corynebacterium glutamicum, revealed by metabolomics.
Woo HM, Noack S, Seibold GM, Willbold S, Eikmanns BJ, Bott M., Appl Environ Microbiol 76(20), 2010
PMID: 20802079
Visualizing post genomics data-sets on customized pathway maps by ProMeTra-aeration-dependent gene expression and metabolism of Corynebacterium glutamicum as an example.
Neuweger H, Persicke M, Albaum SP, Bekel T, Dondrup M, Hüser AT, Winnebald J, Schneider J, Kalinowski J, Goesmann A., BMC Syst Biol 3(), 2009
PMID: 19698148
Engineering of a glycerol utilization pathway for amino acid production by Corynebacterium glutamicum.
Rittmann D, Lindner SN, Wendisch VF., Appl Environ Microbiol 74(20), 2008
PMID: 18757581

52 References

Data provided by Europe PubMed Central.

Targeted disruption of the alpha-amylase gene in the hyperthermophilic archaeon Sulfolobus solfataricus.
Worthington P, Hoang V, Perez-Pomares F, Blum P., J. Bacteriol. 185(2), 2003
PMID: 12511494

Export

0 Marked Publications

Open Data PUB

Web of Science

View record in Web of Science®

Sources

PMID: 16488498
PubMed | Europe PMC

Search this title in

Google Scholar