Transcriptome profiling uncovers metabolic and regulatory processes occurring during the transition from desiccation-sensitive to desiccation-tolerant stages in Medicago truncatula seeds

Buitink J, Leger JJ, Guisle I, Vu BL, Wuilleme S, Lamirault G, Le Bars A, Le Meur N, Becker A, Kuester H, Leprince O (2006)
PLANT JOURNAL 47(5): 735-750.

Download
No fulltext has been uploaded. References only!
Journal Article | Original Article | Published | English

No fulltext has been uploaded

Author
; ; ; ; ; ; ; ; ; ;
Abstract
To investigate regulatory processes and protective mechanisms leading to desiccation tolerance (DT) in seeds, 16086-element microarrays were used to monitor changes in the transcriptome of desiccation-sensitive 3-mm-long radicles of Medicago truncatula seeds at different time points during incubation in a polyethylene glycol (PEG) solution at -1.7 MPa, resulting in a gradual re-establishment of DT. Gene profiling was also performed on embryos before and after the acquisition of DT during maturation. More than 1300 genes were differentially expressed during the PEG incubation. A large number of genes involved in C metabolism are expressed during the re-establishment of DT. Quantification of C reserves confirms that lipids, starch and oligosaccharides were mobilised, coinciding with the production of sucrose during the early osmotic adjustment. Several clusters of gene profiles were identified with different time-scales. Genes expressed early during the PEG incubation belonged to classes involved in early stress and adaptation responses. Interestingly, several regulatory genes typically expressed during abiotic/drought stresses were also upregulated during maturation, arguing for the partial overlap of ABA-dependent and -independent regulatory pathways involved in both drought and DT. At later time points, in parallel to the re-establishment of DT, upregulated genes are comparable with those involved in late seed maturation. Concomitantly, a massive repression of genes belonging to numerous classes occurred, including cell cycle, biogenesis, primary and energy metabolism. The re-establishment of DT in the germinated radicles appears to concur with a partial return to the quiescent state prior to germination.
Publishing Year
ISSN
eISSN
PUB-ID

Cite this

Buitink J, Leger JJ, Guisle I, et al. Transcriptome profiling uncovers metabolic and regulatory processes occurring during the transition from desiccation-sensitive to desiccation-tolerant stages in Medicago truncatula seeds. PLANT JOURNAL. 2006;47(5):735-750.
Buitink, J., Leger, J. J., Guisle, I., Vu, B. L., Wuilleme, S., Lamirault, G., Le Bars, A., et al. (2006). Transcriptome profiling uncovers metabolic and regulatory processes occurring during the transition from desiccation-sensitive to desiccation-tolerant stages in Medicago truncatula seeds. PLANT JOURNAL, 47(5), 735-750. doi:10.1111/j.1365-313X.2006.02822.x
Buitink, J., Leger, J. J., Guisle, I., Vu, B. L., Wuilleme, S., Lamirault, G., Le Bars, A., Le Meur, N., Becker, A., Kuester, H., et al. (2006). Transcriptome profiling uncovers metabolic and regulatory processes occurring during the transition from desiccation-sensitive to desiccation-tolerant stages in Medicago truncatula seeds. PLANT JOURNAL 47, 735-750.
Buitink, J., et al., 2006. Transcriptome profiling uncovers metabolic and regulatory processes occurring during the transition from desiccation-sensitive to desiccation-tolerant stages in Medicago truncatula seeds. PLANT JOURNAL, 47(5), p 735-750.
J. Buitink, et al., “Transcriptome profiling uncovers metabolic and regulatory processes occurring during the transition from desiccation-sensitive to desiccation-tolerant stages in Medicago truncatula seeds”, PLANT JOURNAL, vol. 47, 2006, pp. 735-750.
Buitink, J., Leger, J.J., Guisle, I., Vu, B.L., Wuilleme, S., Lamirault, G., Le Bars, A., Le Meur, N., Becker, A., Kuester, H., Leprince, O.: Transcriptome profiling uncovers metabolic and regulatory processes occurring during the transition from desiccation-sensitive to desiccation-tolerant stages in Medicago truncatula seeds. PLANT JOURNAL. 47, 735-750 (2006).
Buitink, Julia, Leger, Jean J., Guisle, Isabelle, Vu, Benoit Ly, Wuilleme, Sylvie, Lamirault, Guillaume, Le Bars, Alice, Le Meur, Nolwenn, Becker, Anke, Kuester, Helge, and Leprince, Olivier. “Transcriptome profiling uncovers metabolic and regulatory processes occurring during the transition from desiccation-sensitive to desiccation-tolerant stages in Medicago truncatula seeds”. PLANT JOURNAL 47.5 (2006): 735-750.
This data publication is cited in the following publications:
This publication cites the following data publications:

59 Citations in Europe PMC

Data provided by Europe PubMed Central.

From Avicennia to Zizania: seed recalcitrance in perspective.
Berjak P, Pammenter NW., Ann Bot 101(2), 2008
PMID: 17704237
Annexins: multifunctional components of growth and adaptation.
Mortimer JC, Laohavisit A, Macpherson N, Webb A, Brownlee C, Battey NH, Davies JM., J Exp Bot 59(3), 2008
PMID: 18267940
Expressed sequence tag analysis of the response of apple (Malus x domestica'Royal Gala') to low temperature and water deficit.
Wisniewski M, Bassett C, Norelli J, Macarisin D, Artlip T, Gasic K, Korban S., Physiol Plant 133(2), 2008
PMID: 18298416
Deciphering the regulatory mechanisms of abiotic stress tolerance in plants by genomic approaches.
Sreenivasulu N, Sopory SK, Kavi Kishor PB., Gene 388(1-2), 2007
PMID: 17134853
Combined networks regulating seed maturation.
Gutierrez L, Van Wuytswinkel O, Castelain M, Bellini C., Trends Plant Sci 12(7), 2007
PMID: 17588801

52 References

Data provided by Europe PubMed Central.

Translational control in plant stress: the formation of messenger ribonucleoprotein particles (mRNPs) in response to desiccation of Tortula ruralis gametophytes
Wood, Plant J. 18(), 1999

Export

0 Marked Publications

Open Data PUB

Web of Science

View record in Web of Science®

Sources

PMID: 16923015
PubMed | Europe PMC

Search this title in

Google Scholar