Transcriptome profiling reveals the importance of plasmid pSymB for osmoadaptation of Sinorhizobium meliloti

Dominguez-Ferreras A, Perez-Arnedo R, Becker A, Olivares J, Soto MJ, Sanjuan J (2006)
JOURNAL OF BACTERIOLOGY 188(21): 7617-7625.

Journal Article | Published | English

No fulltext has been uploaded

Author
; ; ; ; ;
Abstract
In this work, DNA microarrays were used to investigate genome-wide transcriptional responses of Sinorhi-zobium meliloti to a sudden increase in external osmolarity elicited by addition of either NaCl or sucrose to exponentially growing cultures. A time course of the response within the first 4 h after the osmotic shock was established. We found that there was a general redundancy in the differentially expressed genes after NaCl or sucrose addition. Both kinds of stress resulted in induction of a large number of genes having unknown functions and in repression of many genes coding for proteins with known functions. There was a strong replicon bias in the pattern of the osmotic stress response; whereas 64% of the upregulated genes had a plasmid localization, 85% of the downregulated genes were chromosomal. Among the pSymB osmoresponsive genes, 83% were upregulated, suggesting the importance of this plasmid for S. meliloti osmoadaptation. Indeed, we identified a 200-kb region in pSymB needed for adaptation to saline shock which has a high density of osmoregulated genes.
Publishing Year
ISSN
PUB-ID

Cite this

Dominguez-Ferreras A, Perez-Arnedo R, Becker A, Olivares J, Soto MJ, Sanjuan J. Transcriptome profiling reveals the importance of plasmid pSymB for osmoadaptation of Sinorhizobium meliloti. JOURNAL OF BACTERIOLOGY. 2006;188(21):7617-7625.
Dominguez-Ferreras, A., Perez-Arnedo, R., Becker, A., Olivares, J., Soto, M. J., & Sanjuan, J. (2006). Transcriptome profiling reveals the importance of plasmid pSymB for osmoadaptation of Sinorhizobium meliloti. JOURNAL OF BACTERIOLOGY, 188(21), 7617-7625.
Dominguez-Ferreras, A., Perez-Arnedo, R., Becker, A., Olivares, J., Soto, M. J., and Sanjuan, J. (2006). Transcriptome profiling reveals the importance of plasmid pSymB for osmoadaptation of Sinorhizobium meliloti. JOURNAL OF BACTERIOLOGY 188, 7617-7625.
Dominguez-Ferreras, A., et al., 2006. Transcriptome profiling reveals the importance of plasmid pSymB for osmoadaptation of Sinorhizobium meliloti. JOURNAL OF BACTERIOLOGY, 188(21), p 7617-7625.
A. Dominguez-Ferreras, et al., “Transcriptome profiling reveals the importance of plasmid pSymB for osmoadaptation of Sinorhizobium meliloti”, JOURNAL OF BACTERIOLOGY, vol. 188, 2006, pp. 7617-7625.
Dominguez-Ferreras, A., Perez-Arnedo, R., Becker, A., Olivares, J., Soto, M.J., Sanjuan, J.: Transcriptome profiling reveals the importance of plasmid pSymB for osmoadaptation of Sinorhizobium meliloti. JOURNAL OF BACTERIOLOGY. 188, 7617-7625 (2006).
Dominguez-Ferreras, Ana, Perez-Arnedo, Rebeca, Becker, Anke, Olivares, Jose, Soto, Maria J., and Sanjuan, Juan. “Transcriptome profiling reveals the importance of plasmid pSymB for osmoadaptation of Sinorhizobium meliloti”. JOURNAL OF BACTERIOLOGY 188.21 (2006): 7617-7625.
This data publication is cited in the following publications:
This publication cites the following data publications:

51 Citations in Europe PMC

Data provided by Europe PubMed Central.

Gene SMb21071 of plasmid pSymB is required for osmoadaptation of Sinorhizobium meliloti 1021 and is implicated in modifications of cell surface polysaccharides structure in response to hyperosmotic stress.
Reguera M, Lloret J, Margaret I, Vinardell JM, Martin M, Buendia A, Rivilla R, Ruiz-Sainz JE, Bonilla I, Bolanos L., Can. J. Microbiol. 55(10), 2009
PMID: 19935886

67 References

Data provided by Europe PubMed Central.

Polyamine transport and role of potE in response to osmotic stress in Escherichia coli.
Schiller D, Kruse D, Kneifel H, Kramer R, Burkovski A., J. Bacteriol. 182(21), 2000
PMID: 11029450

AUTHOR UNKNOWN, 2001
Effects of salinity on protein and lipopolysaccharide pattern in a salt-tolerant strain of Mesorhizobium ciceri.
Soussi M, Santamaria M, Ocana A, Lluch C., J. Appl. Microbiol. 90(3), 2001
PMID: 11298245
Regulation cascade of flagellar expression in Gram-negative bacteria.
Soutourina OA, Bertin PN., FEMS Microbiol. Rev. 27(4), 2003
PMID: 14550943
Root nodulation and infection factors produced by rhizobial bacteria.
Spaink HP., Annu. Rev. Microbiol. 54(), 2000
PMID: 11018130
Genetics and genomics of root symbiosis.
Stougaard J., Curr. Opin. Plant Biol. 4(4), 2001
PMID: 11418343
Defective O-antigen polymerization in tolA and pal mutants of Escherichia coli in response to extracytoplasmic stress.
Vines ED, Marolda CL, Balachandran A, Valvano MA., J. Bacteriol. 187(10), 2005
PMID: 15866920
The role of RbfA in 16S rRNA processing and cell growth at low temperature in Escherichia coli.
Xia B, Ke H, Shinde U, Inouye M., J. Mol. Biol. 332(3), 2003
PMID: 12963368
Normalization for cDNA microarray data: a robust composite method addressing single and multiple slide systematic variation.
Yang YH, Dudoit S, Luu P, Lin DM, Peng V, Ngai J, Speed TP., Nucleic Acids Res. 30(4), 2002
PMID: 11842121
Combined transcriptome and proteome analysis of Escherichia coli during high cell density culture.
Yoon SH, Han MJ, Lee SY, Jeong KJ, Yoo JS., Biotechnol. Bioeng. 81(7), 2003
PMID: 12557308

Export

0 Marked Publications

Open Data PUB

Web of Science

View record in Web of Science®

Sources

PMID: 16916894
PubMed | Europe PMC

Search this title in

Google Scholar