Heparan sulfate 6-O-endosulfatases: discrete in vivo activities and functional co-operativity

Lamanna WC, Baldwin RJ, Padva M, Kalus I, ten Dam G, van Kuppevelt TH, Gallagher JT, von Figura K, Dierks T, Merry CLR (2006)
BIOCHEMICAL JOURNAL 400(1): 63-73.

Journal Article | Published | English

No fulltext has been uploaded

Author
; ; ; ; ; ; ; ; ;
Abstract
HS (heparan sulfate) is essential for normal embryonic development. This requirement is due to the obligatory role for HS in the signalling pathways of many growth factors and morphogens that bind to sulfated domains in the HS polymer chain. The sulfation patterning of HS is determined by a complex interplay of Golgi-located N- and O-sulfotransferases which sulfate the heparan precursor and cell surface endosulfatases that selectively remove 6-O-sulfates from mature HS chains. In the present study we generated single or double knock-out mice for the two murine endosulfatases mSulf1 and mSulf2. Detailed structural analysis of HS from mSulf1(-/-) fibroblasts showed a striking increase in 6-O-sulfation. which was not seen in mSulf2(-/-) HS. Intriguingly, the level of 6-O-suffation in the double mSulf1(-/-)/2(-/-) HS was significantly higher than that observed in the mSulf1(-/-) counterpart. These data imply that mSulf1 and mSulf2 are functionally co-operative. Unlike their avian orthologues, mammalian Sulf activities are not restricted to the highly sulfated S-domains of HS. Mitogenesis assays with FGF2 (fibroblast growth factor 2) revealed that Sulf activity decreases the activating potential of newly-synthesized HS, suggesting an important role for these enzymes in cell growth regulation in embryonic and adult tissues.
Publishing Year
ISSN
eISSN
PUB-ID

Cite this

Lamanna WC, Baldwin RJ, Padva M, et al. Heparan sulfate 6-O-endosulfatases: discrete in vivo activities and functional co-operativity. BIOCHEMICAL JOURNAL. 2006;400(1):63-73.
Lamanna, W. C., Baldwin, R. J., Padva, M., Kalus, I., ten Dam, G., van Kuppevelt, T. H., Gallagher, J. T., et al. (2006). Heparan sulfate 6-O-endosulfatases: discrete in vivo activities and functional co-operativity. BIOCHEMICAL JOURNAL, 400(1), 63-73.
Lamanna, W. C., Baldwin, R. J., Padva, M., Kalus, I., ten Dam, G., van Kuppevelt, T. H., Gallagher, J. T., von Figura, K., Dierks, T., and Merry, C. L. R. (2006). Heparan sulfate 6-O-endosulfatases: discrete in vivo activities and functional co-operativity. BIOCHEMICAL JOURNAL 400, 63-73.
Lamanna, W.C., et al., 2006. Heparan sulfate 6-O-endosulfatases: discrete in vivo activities and functional co-operativity. BIOCHEMICAL JOURNAL, 400(1), p 63-73.
W.C. Lamanna, et al., “Heparan sulfate 6-O-endosulfatases: discrete in vivo activities and functional co-operativity”, BIOCHEMICAL JOURNAL, vol. 400, 2006, pp. 63-73.
Lamanna, W.C., Baldwin, R.J., Padva, M., Kalus, I., ten Dam, G., van Kuppevelt, T.H., Gallagher, J.T., von Figura, K., Dierks, T., Merry, C.L.R.: Heparan sulfate 6-O-endosulfatases: discrete in vivo activities and functional co-operativity. BIOCHEMICAL JOURNAL. 400, 63-73 (2006).
Lamanna, William C., Baldwin, Rebecca J., Padva, Michael, Kalus, Ina, ten Dam, Gerdy, van Kuppevelt, Toin H., Gallagher, John T., von Figura, Kurt, Dierks, Thomas, and Merry, Catherine L. R. “Heparan sulfate 6-O-endosulfatases: discrete in vivo activities and functional co-operativity”. BIOCHEMICAL JOURNAL 400.1 (2006): 63-73.
This data publication is cited in the following publications:
This publication cites the following data publications:

58 Citations in Europe PMC

Data provided by Europe PubMed Central.

Sulf1 and Sulf2 Differentially Modulate Heparan Sulfate Proteoglycan Sulfation during Postnatal Cerebellum Development: Evidence for Neuroprotective and Neurite Outgrowth Promoting Functions.
Kalus I, Rohn S, Puvirajesinghe TM, Guimond SE, Eyckerman-Kolln PJ, Ten Dam G, van Kuppevelt TH, Turnbull JE, Dierks T., PLoS ONE 10(10), 2015
PMID: 26448642
HSulf-1 deficiency dictates a metabolic reprograming of glycolysis and TCA cycle in ovarian cancer.
Mondal S, Roy D, Camacho-Pereira J, Khurana A, Chini E, Yang L, Baddour J, Stilles K, Padmabandu S, Leung S, Kalloger S, Gilks B, Lowe V, Dierks T, Hammond E, Dredge K, Nagrath D, Shridhar V., Oncotarget 6(32), 2015
PMID: 26378042
Using embryonic stem cells to understand how glycosaminoglycans regulate differentiation.
Holley RJ, Meade KA, Merry CL., Biochem. Soc. Trans. 42(3), 2014
PMID: 24849238
The heparan sulfate editing enzyme Sulf1 plays a novel role in zebrafish VegfA mediated arterial venous identity.
Gorsi B, Liu F, Ma X, Chico TJ, v A, Kramer KL, Bridges E, Monteiro R, Harris AL, Patient R, Stringer SE., Angiogenesis 17(1), 2014
PMID: 23959107
Production methods for heparosan, a precursor of heparin and heparan sulfate.
Chavaroche AA, van den Broek LA, Eggink G., Carbohydr Polym 93(1), 2013
PMID: 23465899

Export

0 Marked Publications

Open Data PUB

Web of Science

View record in Web of Science®

Sources

PMID: 16901266
PubMed | Europe PMC

Search this title in

Google Scholar