Quantitative determination of adrenaline and noradrenaline in urine using liquid chromatography-tandem mass spectrometry

Thomas A, Geyer H, Mester HJ, Schaenzer W, Zimmermann E, Thevis M (2006)
CHROMATOGRAPHIA 64(9-10): 587-591.

Journal Article | Published | English

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Abstract
The quantitative determination of adrenaline (A) and noradrenaline (N) in human urine provides a helpful tool for sports sciences as well as clinical purposes. A rapid and highly specific procedure based on an off-line sample preparation using weak cation exchange solid phase extraction (CX-SPE) followed by liquid chromatography-tandem mass spectrometry (LC-MS-MS) was developed that allowed the unambiguous identification of the target analytes at low (10 ng mL(-1) A and N), medium and high (500 ng mL(-1) A and N) physiological concentration levels. Recovery rates ranged from 104 to 111 %, precisions of better than 15% and a linear approximation in the aimed working range were obtained for both compounds. Thus, this method provides a rugged and highly specific alternative to the commonly used electrochemical or fluorescence detection strategies. In- and out-of-competition catecholamine concentrations in urine samples of six professional German first division soccer players were determined demonstrating an obvious decrease of the N/A-ratio post-competition, compared to pre-competition and out-of-competition values providing a parameter for the estimation of physiological and psychological stress.
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Thomas A, Geyer H, Mester HJ, Schaenzer W, Zimmermann E, Thevis M. Quantitative determination of adrenaline and noradrenaline in urine using liquid chromatography-tandem mass spectrometry. CHROMATOGRAPHIA. 2006;64(9-10):587-591.
Thomas, A., Geyer, H., Mester, H. J., Schaenzer, W., Zimmermann, E., & Thevis, M. (2006). Quantitative determination of adrenaline and noradrenaline in urine using liquid chromatography-tandem mass spectrometry. CHROMATOGRAPHIA, 64(9-10), 587-591.
Thomas, A., Geyer, H., Mester, H. J., Schaenzer, W., Zimmermann, E., and Thevis, M. (2006). Quantitative determination of adrenaline and noradrenaline in urine using liquid chromatography-tandem mass spectrometry. CHROMATOGRAPHIA 64, 587-591.
Thomas, A., et al., 2006. Quantitative determination of adrenaline and noradrenaline in urine using liquid chromatography-tandem mass spectrometry. CHROMATOGRAPHIA, 64(9-10), p 587-591.
A. Thomas, et al., “Quantitative determination of adrenaline and noradrenaline in urine using liquid chromatography-tandem mass spectrometry”, CHROMATOGRAPHIA, vol. 64, 2006, pp. 587-591.
Thomas, A., Geyer, H., Mester, H.J., Schaenzer, W., Zimmermann, E., Thevis, M.: Quantitative determination of adrenaline and noradrenaline in urine using liquid chromatography-tandem mass spectrometry. CHROMATOGRAPHIA. 64, 587-591 (2006).
Thomas, Andreas, Geyer, H., Mester, H. J., Schaenzer, W., Zimmermann, Elke, and Thevis, M. “Quantitative determination of adrenaline and noradrenaline in urine using liquid chromatography-tandem mass spectrometry”. CHROMATOGRAPHIA 64.9-10 (2006): 587-591.
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