Complementation analyses of Sinorhizobium meliloti nifA mutant with different originated nifA genes

Yao Z, Tian Z, Dai X, Becker A, Li J, Yan H, Xiao Y, Zhu J, Yu G, Rueverg S, Wang Y, et al. (2006)
CHINESE SCIENCE BULLETIN 51(22): 2748-2754.

Journal Article | Published | English

No fulltext has been uploaded

Author
; ; ; ; ; ; ; ; ; ; ;
All
Abstract
A previous work inferred that the nifA gene of Enterobacter cloacae did not restore the symbiotic phenotype of Sinorhizobium meliloti nifA mutant. In the present study, two nifA genes of Bradyrhizobiurn japonicum and Mesorhizobium huakuii also did not restore the symbiotic phenotype of S. meliloti nifA mutant. In whole genomic microarray experiments, 238 genes were found to be differentially expressed after S. meliloti nifA had been constitutively expressed in its nifA mutant. In contrast, only 20, 7 and 9 genes changed their transcriptional levels when expressing B. japonium, M. huakuii and Enterobacter cloacae nifA genes in Sm nifA mutant separately. These genes were classified into severai functional groups including house keeping, energy and central intermediary metabolism, transport systems and symbiosis. Interestingly, the genes that of nifH operons showed high expression levels in the presence of either B. japonium or M. huakuii NifA, which was confirmed by subsequent lacZ fusion experiments.
Publishing Year
ISSN
eISSN
PUB-ID

Cite this

Yao Z, Tian Z, Dai X, et al. Complementation analyses of Sinorhizobium meliloti nifA mutant with different originated nifA genes. CHINESE SCIENCE BULLETIN. 2006;51(22):2748-2754.
Yao , Z., Tian , Z., Dai , X., Becker, A., Li , J., Yan, H., Xiao, Y., et al. (2006). Complementation analyses of Sinorhizobium meliloti nifA mutant with different originated nifA genes. CHINESE SCIENCE BULLETIN, 51(22), 2748-2754.
Yao , Z., Tian , Z., Dai , X., Becker, A., Li , J., Yan, H., Xiao, Y., Zhu , J., Yu , G., Rueverg, S., et al. (2006). Complementation analyses of Sinorhizobium meliloti nifA mutant with different originated nifA genes. CHINESE SCIENCE BULLETIN 51, 2748-2754.
Yao , Z., et al., 2006. Complementation analyses of Sinorhizobium meliloti nifA mutant with different originated nifA genes. CHINESE SCIENCE BULLETIN, 51(22), p 2748-2754.
Z. Yao, et al., “Complementation analyses of Sinorhizobium meliloti nifA mutant with different originated nifA genes”, CHINESE SCIENCE BULLETIN, vol. 51, 2006, pp. 2748-2754.
Yao , Z., Tian , Z., Dai , X., Becker, A., Li , J., Yan, H., Xiao, Y., Zhu , J., Yu , G., Rueverg, S., Wang , Y., Zou , H.: Complementation analyses of Sinorhizobium meliloti nifA mutant with different originated nifA genes. CHINESE SCIENCE BULLETIN. 51, 2748-2754 (2006).
Yao , Z., Tian , Z., Dai , X., Becker, Anke, Li , J., Yan, H., Xiao, Y., Zhu , J., Yu , G., Rueverg, Silvia, Wang , Y., and Zou , H. “Complementation analyses of Sinorhizobium meliloti nifA mutant with different originated nifA genes”. CHINESE SCIENCE BULLETIN 51.22 (2006): 2748-2754.
This data publication is cited in the following publications:
This publication cites the following data publications:

Export

0 Marked Publications

Open Data PUB

Web of Science

View record in Web of Science®

Search this title in

Google Scholar