GC-MS based metabolite profiling implies three interdependent ways of ammonium assimilation in Medicago truncatula root nodules

Barsch A, Carvalho HG, Cullimore JV, Niehaus K (2006)
Journal of Biotechnology 127(1): 79-83.

Journal Article | Published | English

No fulltext has been uploaded

Author
; ; ;
Abstract
In symbiotic interaction with legume plants, bacteria termed Rhizobia can fix massive amounts of atmospheric nitrogen which is primarily provided in the form of ammonium to the host plants. Therefore, legume root nodules that house the symbiotic bacteria are ideally suited to study the process of primary ammonium assimilation. Here, we present a GC-MS based metabolite profiling analysis of Medicago truncatula root nodules (induced by the bacterium Sinorhizobium meliloti) before and after inhibition of glutamine synthetase (GS) by the chemical herbicide phosphinotricine. The primary role of GS in ammonium assimilation was revealed by drastically reduced levels of glutamine in phosphinotricine treated root nodules. In comparison to previous results of increased asparagine synthetase transcript and protein abundances in GS inhibited nodules the metabolic data revealed that decreased amounts of aspartate might preclude taking advantage of this elevated enzymatic activity. A potential role of glutamate dehydrogenase in ammonium assimilation was metabolically indicated 24 and 48 h after GS inhibition. Therefore, nodule ammonium assimilation might in principle involve three interdependent metabolic pathways which are adjusted to control basic nitrogen metabolism. (c) 2006 Elsevier B.V. All rights reserved.
Publishing Year
ISSN
PUB-ID

Cite this

Barsch A, Carvalho HG, Cullimore JV, Niehaus K. GC-MS based metabolite profiling implies three interdependent ways of ammonium assimilation in Medicago truncatula root nodules. Journal of Biotechnology. 2006;127(1):79-83.
Barsch, A., Carvalho, H. G., Cullimore, J. V., & Niehaus, K. (2006). GC-MS based metabolite profiling implies three interdependent ways of ammonium assimilation in Medicago truncatula root nodules. Journal of Biotechnology, 127(1), 79-83.
Barsch, A., Carvalho, H. G., Cullimore, J. V., and Niehaus, K. (2006). GC-MS based metabolite profiling implies three interdependent ways of ammonium assimilation in Medicago truncatula root nodules. Journal of Biotechnology 127, 79-83.
Barsch, A., et al., 2006. GC-MS based metabolite profiling implies three interdependent ways of ammonium assimilation in Medicago truncatula root nodules. Journal of Biotechnology, 127(1), p 79-83.
A. Barsch, et al., “GC-MS based metabolite profiling implies three interdependent ways of ammonium assimilation in Medicago truncatula root nodules”, Journal of Biotechnology, vol. 127, 2006, pp. 79-83.
Barsch, A., Carvalho, H.G., Cullimore, J.V., Niehaus, K.: GC-MS based metabolite profiling implies three interdependent ways of ammonium assimilation in Medicago truncatula root nodules. Journal of Biotechnology. 127, 79-83 (2006).
Barsch, Aiko, Carvalho, Helena G., Cullimore, Julie V., and Niehaus, Karsten. “GC-MS based metabolite profiling implies three interdependent ways of ammonium assimilation in Medicago truncatula root nodules”. Journal of Biotechnology 127.1 (2006): 79-83.
This data publication is cited in the following publications:
This publication cites the following data publications:

10 Citations in Europe PMC

Data provided by Europe PubMed Central.

A nitrogen-dependent switch in the high affinity ammonium transport in Medicago truncatula.
Straub D, Ludewig U, Neuhauser B., Plant Mol. Biol. 86(4-5), 2014
PMID: 25164101
Molecular and physiological stages of priming: how plants prepare for environmental challenges.
Gamir J, Sanchez-Bel P, Flors V., Plant Cell Rep. 33(12), 2014
PMID: 25113544
MALDI mass spectrometry-assisted molecular imaging of metabolites during nitrogen fixation in the Medicago truncatula-Sinorhizobium meliloti symbiosis.
Ye H, Gemperline E, Venkateshwaran M, Chen R, Delaux PM, Howes-Podoll M, Ane JM, Li L., Plant J. 75(1), 2013
PMID: 23551619
Cessation of photosynthesis in Lotus japonicus leaves leads to reprogramming of nodule metabolism.
Tsikou D, Kalloniati C, Fotelli MN, Nikolopoulos D, Katinakis P, Udvardi MK, Rennenberg H, Flemetakis E., J. Exp. Bot. 64(5), 2013
PMID: 23404899
Inhibition of glutamine synthetase by phosphinothricin leads to transcriptome reprograming in root nodules of Medicago truncatula.
Seabra AR, Pereira PA, Becker JD, Carvalho HG., Mol. Plant Microbe Interact. 25(7), 2012
PMID: 22414438
Identification of uricase as a potential target of plant thioredoxin: Implication in the regulation of nodule development.
Du H, Kim S, Nam KH, Lee MS, Son O, Lee SH, Cheon CI., Biochem. Biophys. Res. Commun. 397(1), 2010
PMID: 20470756
How rhizobial symbionts invade plants: the Sinorhizobium-Medicago model.
Jones KM, Kobayashi H, Davies BW, Taga ME, Walker GC., Nat. Rev. Microbiol. 5(8), 2007
PMID: 17632573

15 References

Data provided by Europe PubMed Central.

Comprehensive metabolite profiling of Sinorhizobium meliloti using gas chromatography-mass spectrometry.
Barsch A, Patschkowski T, Niehaus K., Funct. Integr. Genomics 4(4), 2004
PMID: 15372312
Engineering herbicide resistance in plants by expression of a detoxifying enzyme.
Block MD, Botterman J, Vandewiele M, Dockx J, Thoen C, Gossele V, Movva NR, Thompson C, Montagu MV, Leemans J., EMBO J. 6(9), 1987
PMID: 16453789
Sucrose synthase in legume nodules is essential for nitrogen fixation
Gordon AJ, Minchin FR, James CL, Komina O., Plant Physiol. 120(3), 1999
PMID: 10398723
Nodule formation and function
Gordon, 2001
Does lowering glutamine synthetase activity in nodules modify nitrogen metabolism and growth of Lotus japonicus?
Harrison J, Pou de Crescenzo MA, Sene O, Hirel B., Plant Physiol. 133(1), 2003
PMID: 12970491
Ammonia assimilation
Hirel, 2001
Carbon and nitrogen metabolism in Rhizobia
Kahn, 1998
The action of the 2-amino-4-(methylphosphinyl)-butanoic acid (phosphinothricin) and its 2-0x0-derivative on the metabolism of cyanobacteria and higher plants
Lea, Phytochemistry 23(), 1984
Key role of bacterial NH(4)(+) metabolism in Rhizobium-plant symbiosis.
Patriarca EJ, Tate R, Iaccarino M., Microbiol. Mol. Biol. Rev. 66(2), 2002
PMID: 12040124
Nitrogen assimilation in alfalfa: isolation and characterization of an asparagine synthetase gene showing enhanced expression in root nodules and dark-adapted leaves.
Shi L, Twary SN, Yoshioka H, Gregerson RG, Miller SS, Samac DA, Gantt JS, Unkefer PJ, Vance CP., Plant Cell 9(8), 1997
PMID: 9286111

Export

0 Marked Publications

Open Data PUB

Web of Science

View record in Web of Science®

Sources

PMID: 16870293
PubMed | Europe PMC

Search this title in

Google Scholar