Multicolor single-molecule spectroscopy with alternating laser excitation for the investigation of interactions and dynamics

Ross J, Buschkamp P, Fetting D, Donnermeyer A, Roth CM, Tinnefeld P (2007)
Journal of Physical Chemistry B 111(2): 321-326.

Download
Es wurde kein Volltext hochgeladen. Nur Publikationsnachweis!
Zeitschriftenaufsatz | Veröffentlicht | Englisch
Autor
; ; ; ; ;
Abstract / Bemerkung
We have developed confocal multicolor single-molecule spectroscopy with optimized detection sensitivity on three spectrally distinct channels for the study of biomolecular interactions and FRET between more than two molecules. Using programmable acousto-optical devices as beamsplitter and excitation filter, we overcome some of the limitations of conventional multichroic beamsplitters and implement rapid alternation between three laser lines. This enables to visualize the synthesis of DNA three-way junctions on a single-molecule basis and to resolve seven stoichiometric subpopulations as well as to quantify FRET in the presence of competing energy transfer pathways. Furthermore, the ability to study correlated molecular movements by monitoring several distances within a biomolecular complex simultaneously is demonstrated.
Erscheinungsjahr
Zeitschriftentitel
Journal of Physical Chemistry B
Band
111
Zeitschriftennummer
2
Seite
321-326
ISSN
PUB-ID

Zitieren

Ross J, Buschkamp P, Fetting D, Donnermeyer A, Roth CM, Tinnefeld P. Multicolor single-molecule spectroscopy with alternating laser excitation for the investigation of interactions and dynamics. Journal of Physical Chemistry B. 2007;111(2):321-326.
Ross, J., Buschkamp, P., Fetting, D., Donnermeyer, A., Roth, C. M., & Tinnefeld, P. (2007). Multicolor single-molecule spectroscopy with alternating laser excitation for the investigation of interactions and dynamics. Journal of Physical Chemistry B, 111(2), 321-326. doi:10.1021/jp066082g
Ross, J., Buschkamp, P., Fetting, D., Donnermeyer, A., Roth, C. M., and Tinnefeld, P. (2007). Multicolor single-molecule spectroscopy with alternating laser excitation for the investigation of interactions and dynamics. Journal of Physical Chemistry B 111, 321-326.
Ross, J., et al., 2007. Multicolor single-molecule spectroscopy with alternating laser excitation for the investigation of interactions and dynamics. Journal of Physical Chemistry B, 111(2), p 321-326.
J. Ross, et al., “Multicolor single-molecule spectroscopy with alternating laser excitation for the investigation of interactions and dynamics”, Journal of Physical Chemistry B, vol. 111, 2007, pp. 321-326.
Ross, J., Buschkamp, P., Fetting, D., Donnermeyer, A., Roth, C.M., Tinnefeld, P.: Multicolor single-molecule spectroscopy with alternating laser excitation for the investigation of interactions and dynamics. Journal of Physical Chemistry B. 111, 321-326 (2007).
Ross, Joachim, Buschkamp, Peter, Fetting, Daniel, Donnermeyer, Achim, Roth, Christian M., and Tinnefeld, Philip. “Multicolor single-molecule spectroscopy with alternating laser excitation for the investigation of interactions and dynamics”. Journal of Physical Chemistry B 111.2 (2007): 321-326.

25 Zitationen in Europe PMC

Daten bereitgestellt von Europe PubMed Central.

Oligomerization of the tetramerization domain of p53 probed by two- and three-color single-molecule FRET.
Chung HS, Meng F, Kim JY, McHale K, Gopich IV, Louis JM., Proc Natl Acad Sci U S A 114(33), 2017
PMID: 28760960
Molecular force spectroscopy with a DNA origami-based nanoscopic force clamp.
Nickels PC, Wünsch B, Holzmeister P, Bae W, Kneer LM, Grohmann D, Tinnefeld P, Liedl T., Science 354(6310), 2016
PMID: 27846560
Alternating-laser excitation: single-molecule FRET and beyond.
Hohlbein J, Craggs TD, Cordes T., Chem Soc Rev 43(4), 2014
PMID: 24037326
Fast single-molecule FRET spectroscopy: theory and experiment.
Chung HS, Gopich IV., Phys Chem Chem Phys 16(35), 2014
PMID: 25088495
A starting point for fluorescence-based single-molecule measurements in biomolecular research.
Gust A, Zander A, Gietl A, Holzmeister P, Schulz S, Lalkens B, Tinnefeld P, Grohmann D., Molecules 19(10), 2014
PMID: 25271426
Linking single-molecule blinking to chromophore structure and redox potentials.
Stein IH, Capone S, Smit JH, Baumann F, Cordes T, Tinnefeld P., Chemphyschem 13(4), 2012
PMID: 22162072
Heat shock protein 90's mechanochemical cycle is dominated by thermal fluctuations.
Ratzke C, Berkemeier F, Hugel T., Proc Natl Acad Sci U S A 109(1), 2012
PMID: 22184223
Advances in quantitative FRET-based methods for studying nucleic acids.
Preus S, Wilhelmsson LM., Chembiochem 13(14), 2012
PMID: 22936620
Improved temporal resolution and linked hidden Markov modeling for switchable single-molecule FRET.
Uphoff S, Gryte K, Evans G, Kapanidis AN., Chemphyschem 12(3), 2011
PMID: 21280168
Multicolor single-molecule FRET to explore protein folding and binding.
Gambin Y, Deniz AA., Mol Biosyst 6(9), 2010
PMID: 20601974
Surfing on a new wave of single-molecule fluorescence methods.
Hohlbein J, Gryte K, Heilemann M, Kapanidis AN., Phys Biol 7(3), 2010
PMID: 20686191
Four-color single-molecule fluorescence with noncovalent dye labeling to monitor dynamic multimolecular complexes.
DeRocco V, Anderson T, Piehler J, Erie DA, Weninger K., Biotechniques 49(5), 2010
PMID: 21091445
Single-molecule four-color FRET.
Lee J, Lee S, Ragunathan K, Joo C, Ha T, Hohng S., Angew Chem Int Ed Engl 49(51), 2010
PMID: 21104966
Single-molecule photophysics of oxazines on DNA and its application in a FRET switch.
Vogelsang J, Cordes T, Tinnefeld P., Photochem Photobiol Sci 8(4), 2009
PMID: 19337662
Correlated movement and bending of nucleic acid structures visualized by multicolor single-molecule spectroscopy.
Person B, Stein IH, Steinhauer C, Vogelsang J, Tinnefeld P., Chemphyschem 10(9-10), 2009
PMID: 19499555
High-speed tuning of visible laser wavelength using a nanoimprinted grating optical tunable filter.
Huang NT, Truxal SC, Tung YC, Hsiao A, Takayama S, Kurabayashi K., Appl Phys Lett 95(21), 2009
PMID: 20052314
Advances in single-molecule fluorescence methods for molecular biology.
Joo C, Balci H, Ishitsuka Y, Buranachai C, Ha T., Annu Rev Biochem 77(), 2008
PMID: 18412538
Two-sided fluorescence resonance energy transfer for assessing molecular interactions of up to three distinct species in confocal microscopy.
Fazekas Z, Petrás M, Fábián A, Pályi-Krekk Z, Nagy P, Damjanovich S, Vereb G, Szöllosi J., Cytometry A 73(3), 2008
PMID: 18044751
Nonequilibrium single molecule protein folding in a coaxial mixer.
Hamadani KM, Weiss S., Biophys J 95(1), 2008
PMID: 18339751
Arabidopsis has two functional orthologs of the yeast V-ATPase assembly factor Vma21p.
Neubert C, Graham LA, Black-Maier EW, Coonrod EM, Liu TY, Stierhof YD, Seidel T, Stevens TH, Schumacher K., Traffic 9(10), 2008
PMID: 18694437

Export

Markieren/ Markierung löschen
Markierte Publikationen

Open Data PUB

Web of Science

Dieser Datensatz im Web of Science®

Quellen

PMID: 17214479
PubMed | Europe PMC

Suchen in

Google Scholar