Identification and characterization of a nodH ortholog from the alfalfa-nodulating Or191-like rhizobia

Del Papa MF, Pistorio M, Draghi WO, Lozano MJ, Giusti MA, Medina C, van Dillewijn P, Martinez-Abarca F, Flores BM, Ruiz-Sainz JE, Megias M, et al. (2007)
Molecular Plant - Microbe Interactions 20(2): 138-145.

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Abstract
Nodulation of Medicago sativa (alfalfa) is known to be restricted to Sinorhizobium meliloti and a few other rhizobia that include the poorly characterized isolates related to Rhizobium sp. strain Or191. Distinctive features of the symbiosis between alfalfa and S. meliloti are the marked specificity from the plant to the bacteria and the strict requirement for the presence of sulfated lipochitooligosaccharides (Nod factors [NFs]) at its reducing end. Here, we present evidence of the presence of a functional nodH-encoded NF sulfotransferase in the Or191-like rhizobia. The nodH gene, present in single copy, maps to a high molecular weight megaplasmid. As in S. meliloti, a nodF homolog was identified immediately upstream of nodH that was transcribed in the opposite direction (local synteny). This novel nodH ortholog was cloned and shown to restore both NF sulfation and the Nif(+)Fix(+) phenotypes when introduced into an S. meliloti nodH mutant. Unexpectedly, however, nodH disruption in the Or191-like bacteria did not abolish their ability to nodulate alfalfa, resulting instead in a severely delayed nodulation. In agreement with evidence from other authors, the nodH sequence analysis strongly supports the idea that the Or191-like rhizobia most likely represent a genetic mosaic resulting from the horizontal transfer of symbiotic genes from a sinorhizobial megaplasmid to a not yet clearly identified ancestor.
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Del Papa MF, Pistorio M, Draghi WO, et al. Identification and characterization of a nodH ortholog from the alfalfa-nodulating Or191-like rhizobia. Molecular Plant - Microbe Interactions. 2007;20(2):138-145.
Del Papa, M. F., Pistorio, M., Draghi, W. O., Lozano, M. J., Giusti, M. A., Medina, C., van Dillewijn, P., et al. (2007). Identification and characterization of a nodH ortholog from the alfalfa-nodulating Or191-like rhizobia. Molecular Plant - Microbe Interactions, 20(2), 138-145.
Del Papa, M. F., Pistorio, M., Draghi, W. O., Lozano, M. J., Giusti, M. A., Medina, C., van Dillewijn, P., Martinez-Abarca, F., Flores, B. M., Ruiz-Sainz, J. E., et al. (2007). Identification and characterization of a nodH ortholog from the alfalfa-nodulating Or191-like rhizobia. Molecular Plant - Microbe Interactions 20, 138-145.
Del Papa, M.F., et al., 2007. Identification and characterization of a nodH ortholog from the alfalfa-nodulating Or191-like rhizobia. Molecular Plant - Microbe Interactions, 20(2), p 138-145.
M.F. Del Papa, et al., “Identification and characterization of a nodH ortholog from the alfalfa-nodulating Or191-like rhizobia”, Molecular Plant - Microbe Interactions, vol. 20, 2007, pp. 138-145.
Del Papa, M.F., Pistorio, M., Draghi, W.O., Lozano, M.J., Giusti, M.A., Medina, C., van Dillewijn, P., Martinez-Abarca, F., Flores, B.M., Ruiz-Sainz, J.E., Megias, M., Pühler, A., Niehaus, K., Toro, N., Lagares, A.: Identification and characterization of a nodH ortholog from the alfalfa-nodulating Or191-like rhizobia. Molecular Plant - Microbe Interactions. 20, 138-145 (2007).
Del Papa, M. F., Pistorio, M., Draghi, W. O., Lozano, M. J., Giusti, M. A., Medina, C., van Dillewijn, P., Martinez-Abarca, F., Flores, B. Moron, Ruiz-Sainz, J. E., Megias, M., Pühler, Alfred, Niehaus, Karsten, Toro, N., and Lagares, A. “Identification and characterization of a nodH ortholog from the alfalfa-nodulating Or191-like rhizobia”. Molecular Plant - Microbe Interactions 20.2 (2007): 138-145.
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25 References

Data provided by Europe PubMed Central.

Dissecting Symbiosis: Developments in Nod Factor Signal Transduction
Oldroyd, Annals of Botany 87(6), 2001
Comparison of drought tolerance in nitrogen-fixing and inorganic nitrogen-grown common beans.
Lodeiro AR, Gonzalez P, Hernandez A, Balague LJ, Favelukes G., Plant Sci. 154(1), 2000
PMID: 10725556
Root nodulation and infection factors produced by rhizobial bacteria.
Spaink HP., Annu. Rev. Microbiol. 54(), 2000
PMID: 11018130
Molecular basis of symbiotic promiscuity.
Perret X, Staehelin C, Broughton WJ., Microbiol. Mol. Biol. Rev. 64(1), 2000
PMID: 10704479
In vitro sulfotransferase activity of NoeE, a nodulation protein of Rhizobium sp. NGR234.
Quesada-Vincens D, Hanin M, Broughton WJ, Jabbouri S., Mol. Plant Microbe Interact. 11(7), 1998
PMID: 9650293
Bradyrhizobium sp. Strains that nodulate the leguminous tree Acacia albida produce fucosylated and partially sulfated nod factors.
Ferro M, Lorquin J, Ba S, Sanon K, Prome JC, Boivin C., Appl. Environ. Microbiol. 66(11), 2000
PMID: 11055966
Sulphation of Rhizobium sp. NGR234 Nod factors is dependent on noeE, a new host-specificity gene.
Hanin M, Jabbouri S, Quesada-Vincens D, Freiberg C, Perret X, Prome JC, Broughton WJ, Fellay R., Mol. Microbiol. 24(6), 1997
PMID: 9218762
Molecular basis of symbiotic host specificity in Rhizobium meliloti: nodH and nodPQ genes encode the sulfation of lipo-oligosaccharide signals.
Roche P, Debelle F, Maillet F, Lerouge P, Faucher C, Truchet G, Denarie J, Prome JC., Cell 67(6), 1991
PMID: 1760841
Organization, structure and symbiotic function of Rhizobium meliloti nodulation genes determining host specificity for alfalfa.
Horvath B, Kondorosi E, John M, Schmidt J, Torok I, Gyorgypal Z, Barabas I, Wieneke U, Schell J, Kondorosi A., Cell 46(3), 1986
PMID: 3731273
Induction of nodule primordia on Phaseolus and Acacia by lipo-chitin oligosaccharide nodulation signals from broad-host-range Rhizobium strain GRH2.
Lopez-Lara IM, van der Drift KM, van Brussel AA, Haverkamp J, Lugtenberg BJ, Thomas-Oates JE, Spaink HP., Plant Mol. Biol. 29(3), 1995
PMID: 8534846
Broad host range DNA cloning system for gram-negative bacteria: construction of a gene bank of Rhizobium meliloti.
Ditta G, Stanfield S, Corbin D, Helinski DR., Proc. Natl. Acad. Sci. U.S.A. 77(12), 1980
PMID: 7012838
Rhizobium meliloti produces a family of sulfated lipooligosaccharides exhibiting different degrees of plant host specificity.
Schultze M, Quiclet-Sire B, Kondorosi E, Virelizer H, Glushka JN, Endre G, Gero SD, Kondorosi A., Proc. Natl. Acad. Sci. U.S.A. 89(1), 1992
PMID: 1729688
Identification of a third sulfate activation system in Sinorhizobium sp. strain BR816: the CysDN sulfate activation complex.
Snoeck C, Verreth C, Hernandez-Lucas I, Martinez-Romero E, Vanderleyden J., Appl. Environ. Microbiol. 69(4), 2003
PMID: 12676676
Symbiotic host-specificity of Rhizobium meliloti is determined by a sulphated and acylated glucosamine oligosaccharide signal.
Lerouge P, Roche P, Faucher C, Maillet F, Truchet G, Prome JC, Denarie J., Nature 344(6268), 1990
PMID: 2330031
Sulphated lipo-oligosaccharide signals of Rhizobium meliloti elicit root nodule organogenesis in alfalfa
Truchet, Nature 351(6328), 1991
Rhizobium--plant signal exchange.
Fisher RF, Long SR., Nature 357(6380), 1992
PMID: 1614514
A Broad Host Range Mobilization System for In Vivo Genetic Engineering: Transposon Mutagenesis in Gram Negative Bacteria
Simon, Bio/Technology 1(9), 1983
Nitrogen-fixing sinorhizobia with Medicago laciniata constitute a novel biovar (bv. medicaginis) of S. meliloti.
Villegas Mdel C, Rome S, Maure L, Domergue O, Gardan L, Bailly X, Cleyet-Marel JC, Brunel B., Syst. Appl. Microbiol. 29(7), 2006
PMID: 16413160

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