Novel macrolide resistance module carried by the IncP-1 beta resistance plasmid pRSB111, isolated from a wastewater treatment plant

Szczepanowski R, Krahn I, Bohn N, Pühler A, Schlueter A (2007)
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY 51(2): 673-678.

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Abstract
The macrolide resistance plasmid pRSB111 was isolated from bacteria residing in the final effluents of a wastewater treatment plant. The 47-kb plasmid confers resistance to azithromycin, clarithromycin, erythromycin, roxithromycin, and tylosin when it is carried by Pseudomonas sp. strain B13 and is very similar to prototype IncP-1 beta plasmid pB3, which was previously isolated from an activated-sludge bacterial community of a wastewater treatment plant. The two plasmids differ in their accessory regions, located downstream of the conjugative transfer module gene traC. Nucleotide sequence analysis of the pRSB111 accessory region revealed that it contains a new macrolide resistance module composed of the genes mphR(E), mph(E), and mrx(E), which putatively encode a transcriptional regulator, a macrolide phosphotransferase, and a transmembrane transport protein, respectively. Analysis of the contributions of the individual genes of the macrolide resistance module revealed that mph(E) and mrx(E) are required for high-level macrolide resistance. The resistance genes are flanked by two insertion sequences, namely, 1SPa15 and ISRSB111. Two truncated transposable elements, IS6100 and remnants of a Tn3-like transposon, were identified in the vicinity of ISRSB111. The accessory element of pRSB111 apparently replaced the Tn402-like element present on the sister plasmid, pB3, as suggested by the conservation of Tn402-specific terminal inverted repeats on pRSB111.
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Szczepanowski R, Krahn I, Bohn N, Pühler A, Schlueter A. Novel macrolide resistance module carried by the IncP-1 beta resistance plasmid pRSB111, isolated from a wastewater treatment plant. ANTIMICROBIAL AGENTS AND CHEMOTHERAPY. 2007;51(2):673-678.
Szczepanowski, R., Krahn, I., Bohn, N., Pühler, A., & Schlueter, A. (2007). Novel macrolide resistance module carried by the IncP-1 beta resistance plasmid pRSB111, isolated from a wastewater treatment plant. ANTIMICROBIAL AGENTS AND CHEMOTHERAPY, 51(2), 673-678.
Szczepanowski, R., Krahn, I., Bohn, N., Pühler, A., and Schlueter, A. (2007). Novel macrolide resistance module carried by the IncP-1 beta resistance plasmid pRSB111, isolated from a wastewater treatment plant. ANTIMICROBIAL AGENTS AND CHEMOTHERAPY 51, 673-678.
Szczepanowski, R., et al., 2007. Novel macrolide resistance module carried by the IncP-1 beta resistance plasmid pRSB111, isolated from a wastewater treatment plant. ANTIMICROBIAL AGENTS AND CHEMOTHERAPY, 51(2), p 673-678.
R. Szczepanowski, et al., “Novel macrolide resistance module carried by the IncP-1 beta resistance plasmid pRSB111, isolated from a wastewater treatment plant”, ANTIMICROBIAL AGENTS AND CHEMOTHERAPY, vol. 51, 2007, pp. 673-678.
Szczepanowski, R., Krahn, I., Bohn, N., Pühler, A., Schlueter, A.: Novel macrolide resistance module carried by the IncP-1 beta resistance plasmid pRSB111, isolated from a wastewater treatment plant. ANTIMICROBIAL AGENTS AND CHEMOTHERAPY. 51, 673-678 (2007).
Szczepanowski, Rafael, Krahn, Irene, Bohn, Nadine, Pühler, Alfred, and Schlueter, Andreas. “Novel macrolide resistance module carried by the IncP-1 beta resistance plasmid pRSB111, isolated from a wastewater treatment plant”. ANTIMICROBIAL AGENTS AND CHEMOTHERAPY 51.2 (2007): 673-678.
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Data provided by Europe PubMed Central.

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Chen Z, Fang H, Wang L, Sun F, Wang Y, Yin Z, Yang H, Yang W, Wang J, Xia P, Zhou D, Liu C., Sci Rep 4(), 2014
PMID: 25428613
The TetR family of regulators.
Cuthbertson L, Nodwell JR., Microbiol. Mol. Biol. Rev. 77(3), 2013
PMID: 24006471
Human health implications of clinically relevant bacteria in wastewater habitats.
Varela AR, Manaia CM., Environ Sci Pollut Res Int 20(6), 2013
PMID: 23508533
Urban wastewater treatment plants as hotspots for antibiotic resistant bacteria and genes spread into the environment: a review.
Rizzo L, Manaia C, Merlin C, Schwartz T, Dagot C, Ploy MC, Michael I, Fatta-Kassinos D., Sci. Total Environ. 447(), 2013
PMID: 23396083
IncP-1β plasmids of Comamonas sp. and Delftia sp. strains isolated from a wastewater treatment plant mediate resistance to and decolorization of the triphenylmethane dye crystal violet.
Stolze Y, Eikmeyer F, Wibberg D, Brandis G, Karsten C, Krahn I, Schneiker-Bekel S, Viehover P, Barsch A, Keck M, Top EM, Niehaus K, Schluter A., Microbiology (Reading, Engl.) 158(Pt 8), 2012
PMID: 22653947
Analysis of antibiotic resistance regions in Gram-negative bacteria.
Partridge SR., FEMS Microbiol. Rev. 35(5), 2011
PMID: 21564142
Detection of 140 clinically relevant antibiotic-resistance genes in the plasmid metagenome of wastewater treatment plant bacteria showing reduced susceptibility to selected antibiotics.
Szczepanowski R, Linke B, Krahn I, Gartemann KH, Gutzkow T, Eichler W, Puhler A, Schluter A., Microbiology (Reading, Engl.) 155(Pt 7), 2009
PMID: 19389756
Structure and function of the macrolide biosensor protein, MphR(A), with and without erythromycin.
Zheng J, Sagar V, Smolinsky A, Bourke C, LaRonde-LeBlanc N, Cropp TA., J. Mol. Biol. 387(5), 2009
PMID: 19265703
Genetic diversity and composition of a plasmid metagenome from a wastewater treatment plant.
Schluter A, Krause L, Szczepanowski R, Goesmann A, Puhler A., J. Biotechnol. 136(1-2), 2008
PMID: 18603322
Insight into the plasmid metagenome of wastewater treatment plant bacteria showing reduced susceptibility to antimicrobial drugs analysed by the 454-pyrosequencing technology.
Szczepanowski R, Bekel T, Goesmann A, Krause L, Kromeke H, Kaiser O, Eichler W, Puhler A, Schluter A., J. Biotechnol. 136(1-2), 2008
PMID: 18586057
IncP-1-beta plasmid pGNB1 isolated from a bacterial community from a wastewater treatment plant mediates decolorization of triphenylmethane dyes.
Schluter A, Krahn I, Kollin F, Bonemann G, Stiens M, Szczepanowski R, Schneiker S, Puhler A., Appl. Environ. Microbiol. 73(20), 2007
PMID: 17675426

46 References

Data provided by Europe PubMed Central.

Structure-activity relationships for three macrolide antibiotics in Haemophilus influenzae.
Mabe S, Eller J, Champney WS., Curr. Microbiol. 49(4), 2004
PMID: 15386112
R-plasmid transfer in a wastewater treatment plant.
Mach PA, Grimes DJ., Appl. Environ. Microbiol. 44(6), 1982
PMID: 6760813
Antibiotic resistance in microbes.
Mazel D, Davies J., Cell. Mol. Life Sci. 56(9-10), 1999
PMID: 11212334
GenDB--an open source genome annotation system for prokaryote genomes.
Meyer F, Goesmann A, McHardy AC, Bartels D, Bekel T, Clausen J, Kalinowski J, Linke B, Rupp O, Giegerich R, Puhler A., Nucleic Acids Res. 31(8), 2003
PMID: 12682369
Complete nucleotide sequence of the conjugative tetracycline resistance plasmid pFBAOT6, a member of a group of IncU plasmids with global ubiquity.
Rhodes G, Parkhill J, Bird C, Ambrose K, Jones MC, Huys G, Swings J, Pickup RW., Appl. Environ. Microbiol. 70(12), 2004
PMID: 15574953
Nomenclature for macrolide and macrolide-lincosamide-streptogramin B resistance determinants.
Roberts MC, Sutcliffe J, Courvalin P, Jensen LB, Rood J, Seppala H., Antimicrob. Agents Chemother. 43(12), 1999
PMID: 10582867

AUTHOR UNKNOWN, 1989
Macrolides and airway inflammation in children.
Shinkai M, Rubin BK., Paediatr Respir Rev 6(3), 2005
PMID: 16153572

AUTHOR UNKNOWN, 1998
Detection of erythromycin-resistant determinants by PCR.
Sutcliffe J, Grebe T, Tait-Kamradt A, Wondrack L., Antimicrob. Agents Chemother. 40(11), 1996
PMID: 8913465
Occurrence of integron-associated resistance gene cassettes located on antibiotic resistance plasmids isolated from a wastewater treatment plant.
Tennstedt T, Szczepanowski R, Braun S, Puhler A, Schluter A., FEMS Microbiol. Ecol. 45(3), 2003
PMID: 19719593
Macrolide resistance conferred by base substitutions in 23S rRNA.
Vester B, Douthwaite S., Antimicrob. Agents Chemother. 45(1), 2001
PMID: 11120937
Review of macrolides and ketolides: focus on respiratory tract infections.
Zhanel GG, Dueck M, Hoban DJ, Vercaigne LM, Embil JM, Gin AS, Karlowsky JA., Drugs 61(4), 2001
PMID: 11324679

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