In situ dark field microscopy for on-line monitoring of yeast cultures

Wei N, You J, Friehs K, Flaschel E, Nattkemper TW (2007)
BIOTECHNOLOGY LETTERS 29(3): 373-378.

Journal Article | Published | English

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Abstract
A new-type in situ probe has been developed to acquire dark field images of yeast in bioreactors. It has been derived from an in situ bright field microscope that is able to measure cell density in bioreactors during fermentation processes. The illumination part of the probe has been replaced with a dark field device, in which an aspheric condenser is used, so that high contrast dark field images can be obtained. The technique of second imaging is implemented to improve the sharpness of the images by means of a relay lens. This new in situ probe is expected to enable the evaluation of the cell viability without staining owing to modern image processing.
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Wei N, You J, Friehs K, Flaschel E, Nattkemper TW. In situ dark field microscopy for on-line monitoring of yeast cultures. BIOTECHNOLOGY LETTERS. 2007;29(3):373-378.
Wei, N., You, J., Friehs, K., Flaschel, E., & Nattkemper, T. W. (2007). In situ dark field microscopy for on-line monitoring of yeast cultures. BIOTECHNOLOGY LETTERS, 29(3), 373-378.
Wei, N., You, J., Friehs, K., Flaschel, E., and Nattkemper, T. W. (2007). In situ dark field microscopy for on-line monitoring of yeast cultures. BIOTECHNOLOGY LETTERS 29, 373-378.
Wei, N., et al., 2007. In situ dark field microscopy for on-line monitoring of yeast cultures. BIOTECHNOLOGY LETTERS, 29(3), p 373-378.
N. Wei, et al., “In situ dark field microscopy for on-line monitoring of yeast cultures”, BIOTECHNOLOGY LETTERS, vol. 29, 2007, pp. 373-378.
Wei, N., You, J., Friehs, K., Flaschel, E., Nattkemper, T.W.: In situ dark field microscopy for on-line monitoring of yeast cultures. BIOTECHNOLOGY LETTERS. 29, 373-378 (2007).
Wei, Ning, You, Jia, Friehs, Karl, Flaschel, Erwin, and Nattkemper, Tim Wilhelm. “In situ dark field microscopy for on-line monitoring of yeast cultures”. BIOTECHNOLOGY LETTERS 29.3 (2007): 373-378.
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Bluma A, Hopfner T, Lindner P, Rehbock C, Beutel S, Riechers D, Hitzmann B, Scheper T., Anal Bioanal Chem 398(6), 2010
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A review of non-invasive optical-based image analysis systems for continuous bioprocess monitoring.
Hopfner T, Bluma A, Rudolph G, Lindner P, Scheper T., Bioprocess Biosyst Eng 33(2), 2010
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