Dielectrophoretic manipulation of DNA: Separation and polarizability

Regtmeier J, Duong TT, Eichhorn R, Anselmetti D, Ros A (2007)
Analytical Chemistry 79(10): 3925-3932.

Download
Es wurde kein Volltext hochgeladen. Nur Publikationsnachweis!
Zeitschriftenaufsatz | Veröffentlicht | Englisch
Autor
; ; ; ;
Abstract / Bemerkung
Although separation of polymers based on the combination of dielectrophoretic trapping and electrophoretic forces was proposed 15 years ago, experimental proof has not yet been reported. Here, we address this problem for long DNA fragments in a simple and easy-to-fabricate microfluidic device, in which the DNA is manipulated by electrophoresis and by electrodeless dielectrophoresis. By slowly increasing the strength of the dielectrophoretic traps in the course of the separation experiments, we are able to perform efficient and fast DNA separation according to length for two different DNA conformations: linear DNA (lambda (48.5-kbp) and T2 (164-kbp) DNA) and supercoiled covalently closed circular plasmid DNA (7 and 14 kbp). The underlying migration mechanismthermally induced escape processes out of the dielectrophoretic traps in the direction of the electrophoretic forceis sensitive to different DNA fragments because of length-dependent DNA polarizabilities. This is analyzed in a second series of experiments, where the migration mechanism is exploited for the quantitative measurement of the DNA polarizabilities. This new and simple technique allows for the systematic characterization of the polarizability not only for DNA but also for other biomolecules like proteins. Furthermore, our results have direct implications to future biotechnological applications such as gene therapy and DNA vaccination.
Erscheinungsjahr
Zeitschriftentitel
Analytical Chemistry
Band
79
Zeitschriftennummer
10
Seite
3925-3932
ISSN
eISSN
PUB-ID

Zitieren

Regtmeier J, Duong TT, Eichhorn R, Anselmetti D, Ros A. Dielectrophoretic manipulation of DNA: Separation and polarizability. Analytical Chemistry. 2007;79(10):3925-3932.
Regtmeier, J., Duong, T. T., Eichhorn, R., Anselmetti, D., & Ros, A. (2007). Dielectrophoretic manipulation of DNA: Separation and polarizability. Analytical Chemistry, 79(10), 3925-3932. doi:10.1021/ac062431r
Regtmeier, J., Duong, T. T., Eichhorn, R., Anselmetti, D., and Ros, A. (2007). Dielectrophoretic manipulation of DNA: Separation and polarizability. Analytical Chemistry 79, 3925-3932.
Regtmeier, J., et al., 2007. Dielectrophoretic manipulation of DNA: Separation and polarizability. Analytical Chemistry, 79(10), p 3925-3932.
J. Regtmeier, et al., “Dielectrophoretic manipulation of DNA: Separation and polarizability”, Analytical Chemistry, vol. 79, 2007, pp. 3925-3932.
Regtmeier, J., Duong, T.T., Eichhorn, R., Anselmetti, D., Ros, A.: Dielectrophoretic manipulation of DNA: Separation and polarizability. Analytical Chemistry. 79, 3925-3932 (2007).
Regtmeier, Jan, Duong, Thanh Tu, Eichhorn, Ralf, Anselmetti, Dario, and Ros, Alexandra. “Dielectrophoretic manipulation of DNA: Separation and polarizability”. Analytical Chemistry 79.10 (2007): 3925-3932.

41 Zitationen in Europe PMC

Daten bereitgestellt von Europe PubMed Central.

Dielectrophoretic platforms for bio-microfluidic systems.
Khoshmanesh K, Nahavandi S, Baratchi S, Mitchell A, Kalantar-zadeh K., Biosens Bioelectron 26(5), 2011
PMID: 20933384
Dielectrophoretic mobility determination in DC insulator-based dielectrophoresis.
Weiss NG, Jones PV, Mahanti P, Chen KP, Taylor TJ, Hayes MA., Electrophoresis 32(17), 2011
PMID: 21823129
Physisorbed surface coatings for poly(dimethylsiloxane) and quartz microfluidic devices.
Viefhues M, Manchanda S, Chao TC, Anselmetti D, Regtmeier J, Ros A., Anal Bioanal Chem 401(7), 2011
PMID: 21847528
Dielectrophoresis for manipulation of micro/nano particles in microfluidic systems.
Zhang C, Khoshmanesh K, Mitchell A, Kalantar-Zadeh K., Anal Bioanal Chem 396(1), 2010
PMID: 19578834
DNA manipulation, sorting, and mapping in nanofluidic systems.
Levy SL, Craighead HG., Chem Soc Rev 39(3), 2010
PMID: 20179829
Controlled microparticle manipulation employing low frequency alternating electric fields in an array of insulators.
Baylon-Cardiel JL, Jesús-Pérez NM, Chávez-Santoscoy AV, Lapizco-Encinas BH., Lab Chip 10(23), 2010
PMID: 20936247
Simple replication methods for producing nanoslits in thermoplastics and the transport dynamics of double-stranded DNA through these slits.
Chantiwas R, Hupert ML, Pullagurla SR, Balamurugan S, Tamarit-López J, Park S, Datta P, Goettert J, Cho YK, Soper SA., Lab Chip 10(23), 2010
PMID: 20938506
Manipulation of biological samples using micro and nano techniques.
Castillo J, Dimaki M, Svendsen WE., Integr Biol (Camb) 1(1), 2009
PMID: 20023789
Bioanalytical separations using electric field gradient techniques.
Meighan MM, Staton SJ, Hayes MA., Electrophoresis 30(5), 2009
PMID: 19197905
Directing Brownian motion on a periodic surface.
Speer D, Eichhorn R, Reimann P., Phys Rev Lett 102(12), 2009
PMID: 19392282
Current control in a tilted washboard potential via time-delayed feedback.
Hennig D., Phys Rev E Stat Nonlin Soft Matter Phys 79(4 pt 1), 2009
PMID: 19518180
DNA manipulation by means of insulator-based dielectrophoresis employing direct current electric fields.
Gallo-Villanueva RC, Rodríguez-López CE, Díaz-de-la-Garza RI, Reyes-Betanzo C, Lapizco-Encinas BH., Electrophoresis 30(24), 2009
PMID: 20013902
Size-dependent trajectories of DNA macromolecules due to insulative dielectrophoresis in submicrometer-deep fluidic channels.
Parikesit GO, Markesteijn AP, Piciu OM, Bossche A, Westerweel J, Young IT, Garini Y., Biomicrofluidics 2(2), 2008
PMID: 19693406

Export

Markieren/ Markierung löschen
Markierte Publikationen

Open Data PUB

Web of Science

Dieser Datensatz im Web of Science®

Quellen

PMID: 17444613
PubMed | Europe PMC

Suchen in

Google Scholar