The Ic1R-type transcriptional repressor LtbR regulates the expression of leucine and tryptophan biosynthesis genes in the amino acid producer Corynebacterium glutamicum

Brune I, Jochmann N, Brinkrolf K, Hueser AT, Gerstmeir R, Eikmanns BJ, Kalinowski J, Pühler A, Tauch A (2007)
JOURNAL OF BACTERIOLOGY 189(7): 2720-2733.

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Zeitschriftenaufsatz | Veröffentlicht | Englisch
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Abstract / Bemerkung
The transcriptional regulator Cg1486 of Corynebacterium glutamicum ATCC 13032 is a member of the IclR protein family and belongs to the conserved set of regulatory proteins in corynebacteria. A defined deletion in the cg1486 gene, now designated ltbR (leucine and tryptophan biosynthesis regulator), led to the mutant strain C. glutamicum IB1486. According to whole-genome expression analysis by DNA microarray hybridizations, transcription of the leuB and leuCD genes encoding enzymes of the leucine biosynthesis pathway was enhanced in C. glutamicum IB1486 compared with the wild-type strain. Moreover, the genes of the trpEGDCFBA operon involved in tryptophan biosynthesis of C. glutamicum showed an enhanced expression in the cg1486 mutant strain. Bioinformatics pattern searches in the upstream regions of the differentially expressed genes revealed the common 12-bp motif CA(T/C)ATAGTG(A/G)GA that is located downstream of the -10 region of the mapped promoter sequences. DNA band shift assays with a streptavidin-tagged LtbR protein demonstrated the specific binding of the purified protein to 40-mers containing the 12-bp motif localized in front of leuB, leuC, and trpE, thereby confirming the direct regulatory role of LtbR in the expression of the leucine and tryptophan biosynthesis pathway genes of C. glutamicum. Genes homologous with ltbR were detected upstream of the leuCD genes in almost all sequenced genomes of bacteria belonging to the taxonomic class Actinobacteria. The ltbR-like genes of Corynebacterium diphtheriae, Corynebacterium jeikeium, Mycobacterium bovis, and Bifidobacterium longum were cloned and shown to complement the deregulation of leuB, leuCD, and trpE gene expression in C. glutamicum IB1486.
Erscheinungsjahr
Zeitschriftentitel
JOURNAL OF BACTERIOLOGY
Band
189
Zeitschriftennummer
7
Seite
2720-2733
ISSN
PUB-ID

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Brune I, Jochmann N, Brinkrolf K, et al. The Ic1R-type transcriptional repressor LtbR regulates the expression of leucine and tryptophan biosynthesis genes in the amino acid producer Corynebacterium glutamicum. JOURNAL OF BACTERIOLOGY. 2007;189(7):2720-2733.
Brune, I., Jochmann, N., Brinkrolf, K., Hueser, A. T., Gerstmeir, R., Eikmanns, B. J., Kalinowski, J., et al. (2007). The Ic1R-type transcriptional repressor LtbR regulates the expression of leucine and tryptophan biosynthesis genes in the amino acid producer Corynebacterium glutamicum. JOURNAL OF BACTERIOLOGY, 189(7), 2720-2733. doi:10.1128/JB.01876-06
Brune, I., Jochmann, N., Brinkrolf, K., Hueser, A. T., Gerstmeir, R., Eikmanns, B. J., Kalinowski, J., Pühler, A., and Tauch, A. (2007). The Ic1R-type transcriptional repressor LtbR regulates the expression of leucine and tryptophan biosynthesis genes in the amino acid producer Corynebacterium glutamicum. JOURNAL OF BACTERIOLOGY 189, 2720-2733.
Brune, I., et al., 2007. The Ic1R-type transcriptional repressor LtbR regulates the expression of leucine and tryptophan biosynthesis genes in the amino acid producer Corynebacterium glutamicum. JOURNAL OF BACTERIOLOGY, 189(7), p 2720-2733.
I. Brune, et al., “The Ic1R-type transcriptional repressor LtbR regulates the expression of leucine and tryptophan biosynthesis genes in the amino acid producer Corynebacterium glutamicum”, JOURNAL OF BACTERIOLOGY, vol. 189, 2007, pp. 2720-2733.
Brune, I., Jochmann, N., Brinkrolf, K., Hueser, A.T., Gerstmeir, R., Eikmanns, B.J., Kalinowski, J., Pühler, A., Tauch, A.: The Ic1R-type transcriptional repressor LtbR regulates the expression of leucine and tryptophan biosynthesis genes in the amino acid producer Corynebacterium glutamicum. JOURNAL OF BACTERIOLOGY. 189, 2720-2733 (2007).
Brune, Iris, Jochmann, Nina, Brinkrolf, Karina, Hueser, Andrea T., Gerstmeir, Robert, Eikmanns, Bernhard J., Kalinowski, Jörn, Pühler, Alfred, and Tauch, Andreas. “The Ic1R-type transcriptional repressor LtbR regulates the expression of leucine and tryptophan biosynthesis genes in the amino acid producer Corynebacterium glutamicum”. JOURNAL OF BACTERIOLOGY 189.7 (2007): 2720-2733.

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PMID: 24169948
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PMID: 23800623
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PMID: 22524407
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72 References

Daten bereitgestellt von Europe PubMed Central.

EMBOSS: the European Molecular Biology Open Software Suite.
Rice P, Longden I, Bleasby A., Trends Genet. 16(6), 2000
PMID: 10827456

AUTHOR UNKNOWN, 1989

AUTHOR UNKNOWN, 1992
Comparative analysis of RNA regulatory elements of amino acid metabolism genes in Actinobacteria.
Seliverstov AV, Putzer H, Gelfand MS, Lyubetsky VA., BMC Microbiol. 5(), 2005
PMID: 16202131
Enzymes of the tryptophan synthetic pathway in Brevibacterium flavum.
Sugimoto S, Shiio I., J. Biochem. 81(4), 1977
PMID: 881418
Corynebacterium glutamicum DNA is subjected to methylation-restriction in Escherichia coli.
Tauch A, Kirchner O, Wehmeier L, Kalinowski J, Puhler A., FEMS Microbiol. Lett. 123(3), 1994
PMID: 7988915
Efficient electrotransformation of corynebacterium diphtheriae with a mini-replicon derived from the Corynebacterium glutamicum plasmid pGA1.
Tauch A, Kirchner O, Loffler B, Gotker S, Puhler A, Kalinowski J., Curr. Microbiol. 45(5), 2002
PMID: 12232668
Complete genome sequence and analysis of the multiresistant nosocomial pathogen Corynebacterium jeikeium K411, a lipid-requiring bacterium of the human skin flora.
Tauch A, Kaiser O, Hain T, Goesmann A, Weisshaar B, Albersmeier A, Bekel T, Bischoff N, Brune I, Chakraborty T, Kalinowski J, Meyer F, Rupp O, Schneiker S, Viehoever P, Puhler A., J. Bacteriol. 187(13), 2005
PMID: 15968079

AUTHOR UNKNOWN, 2006
The CLUSTAL_X windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools.
Thompson JD, Gibson TJ, Plewniak F, Jeanmougin F, Higgins DG., Nucleic Acids Res. 25(24), 1997
PMID: 9396791

AUTHOR UNKNOWN, 1978
Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.
Ueda K, Yamashita A, Ishikawa J, Shimada M, Watsuji TO, Morimura K, Ikeda H, Hattori M, Beppu T., Nucleic Acids Res. 32(16), 2004
PMID: 15383646

AUTHOR UNKNOWN, 1996
The DtxR regulon of Corynebacterium glutamicum.
Wennerhold J, Bott M., J. Bacteriol. 188(8), 2006
PMID: 16585752

AUTHOR UNKNOWN, 2005
PredictRegulon: a web server for the prediction of the regulatory protein binding sites and operons in prokaryote genomes.
Yellaboina S, Seshadri J, Kumar MS, Ranjan A., Nucleic Acids Res. 32(Web Server issue), 2004
PMID: 15215402

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