Evaluating high time-resolved changes in carbon isotope ratio of respired CO2 by a rapid in-tube incubation technique

Werner C, Hasenbein N, Maia R, Beyschlag W, Maguas C (2007)
RAPID COMMUNICATIONS IN MASS SPECTROMETRY 21(8): 1352-1360.

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Zeitschriftenaufsatz | Veröffentlicht | Englisch
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Abstract / Bemerkung
Recent insights into fractionation during dark respiration and rapid dynamics in isotope signatures of leaf- and ecosystem-respired CO2 indicate the need for new methods for high time-resolved measurements of the isotopic signature of respired CO2 (delta C-13(res)). We present a rapid and simple method to analyse delta C-13(res) using an in-tube incubation technique and an autosampler for small septum-capped vials. The effect of storage on the delta O-18 and delta C-13 ratios of ambient CO2 concentrations was tested with different humidity and temperatures. delta C-13 ratios remained stable over 72 h, whereas delta O-18 ratios decreased after 24 h. Storage at VC improved the storage time for delta O-18. Leaves or leaf discs were incubated in the vials, flushed with CO2-free air and respired CO2 was automatically sampled within 5 min on a mu Gas autosampler interfaced to a GV-Isoprime isotope ratio mass spectrometer. Results were validated by simultaneous on-line gas-exchange measurements of delta C-13(res) of attached leaves. This method was used to evaluate the short-term (5-60 min) and diurnal dynamics of delta C-13(res) in an evergreen oak (Quercus ilex) and a herb (Tolpis barbata). An immediate depletion of 2-4 parts per thousand from the initial delta C-13(res) value occurred during the first 30 min of darkening. Q. ilex exhibited further a substantial diurnal enrichment in delta C-13(res) of 8 parts per thousand, followed by a progressive depletion during the night. In contrast, T. barbata did not exhibit a distinct diurnal pattern. This is in accordance with recent theory on fractionation in metabolic pathways and may be related to the different utilisation of the respiratory substrate in the fast-growing herb and the evergreen oak. These data indicate substantial and rapid dynamics (within minutes to hours) in delta C-13(res), which differed between species and probably the growth status of the plant. The in-tube incubation method enables both high time-resolved analysis and extensive sampling across different organs, species and functional types. Copyright (C) 2007 John Wiley & Sons, Ltd.
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Zeitschriftentitel
RAPID COMMUNICATIONS IN MASS SPECTROMETRY
Band
21
Zeitschriftennummer
8
Seite
1352-1360
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Werner C, Hasenbein N, Maia R, Beyschlag W, Maguas C. Evaluating high time-resolved changes in carbon isotope ratio of respired CO2 by a rapid in-tube incubation technique. RAPID COMMUNICATIONS IN MASS SPECTROMETRY. 2007;21(8):1352-1360.
Werner, C., Hasenbein, N., Maia, R., Beyschlag, W., & Maguas, C. (2007). Evaluating high time-resolved changes in carbon isotope ratio of respired CO2 by a rapid in-tube incubation technique. RAPID COMMUNICATIONS IN MASS SPECTROMETRY, 21(8), 1352-1360. doi:10.1002/rcm.2970
Werner, C., Hasenbein, N., Maia, R., Beyschlag, W., and Maguas, C. (2007). Evaluating high time-resolved changes in carbon isotope ratio of respired CO2 by a rapid in-tube incubation technique. RAPID COMMUNICATIONS IN MASS SPECTROMETRY 21, 1352-1360.
Werner, C., et al., 2007. Evaluating high time-resolved changes in carbon isotope ratio of respired CO2 by a rapid in-tube incubation technique. RAPID COMMUNICATIONS IN MASS SPECTROMETRY, 21(8), p 1352-1360.
C. Werner, et al., “Evaluating high time-resolved changes in carbon isotope ratio of respired CO2 by a rapid in-tube incubation technique”, RAPID COMMUNICATIONS IN MASS SPECTROMETRY, vol. 21, 2007, pp. 1352-1360.
Werner, C., Hasenbein, N., Maia, R., Beyschlag, W., Maguas, C.: Evaluating high time-resolved changes in carbon isotope ratio of respired CO2 by a rapid in-tube incubation technique. RAPID COMMUNICATIONS IN MASS SPECTROMETRY. 21, 1352-1360 (2007).
Werner, Christiane, Hasenbein, Nils, Maia, Rodrigo, Beyschlag, Wolfram, and Maguas, Cristina. “Evaluating high time-resolved changes in carbon isotope ratio of respired CO2 by a rapid in-tube incubation technique”. RAPID COMMUNICATIONS IN MASS SPECTROMETRY 21.8 (2007): 1352-1360.

26 Zitationen in Europe PMC

Daten bereitgestellt von Europe PubMed Central.

Malate as a key carbon source of leaf dark-respired CO2 across different environmental conditions in potato plants.
Lehmann MM, Rinne KT, Blessing C, Siegwolf RT, Buchmann N, Werner RA., J Exp Bot 66(19), 2015
PMID: 26139821
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Tcherkez G, Schäufele R, Nogués S, Piel C, Boom A, Lanigan G, Barbaroux C, Mata C, Elhani S, Hemming D, Maguas C, Yakir D, Badeck FW, Griffiths H, Schnyder H, Ghashghaie J., Plant Cell Environ 33(6), 2010
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Disentangling drought-induced variation in ecosystem and soil respiration using stable carbon isotopes.
Unger S, Máguas C, Pereira JS, Aires LM, David TS, Werner C., Oecologia 163(4), 2010
PMID: 20217141
Impact of carbohydrate supply on stem growth, wood and respired CO2 delta13C: assessment by experimental girdling.
Maunoury-Danger F, Fresneau C, Eglin T, Berveiller D, François C, Lelarge-Trouverie C, Damesin C., Tree Physiol 30(7), 2010
PMID: 20504776
On the use of phloem sap δ¹³C as an indicator of canopy carbon discrimination.
Rascher KG, Máguas C, Werner C., Tree Physiol 30(12), 2010
PMID: 21071770
Stable carbon isotopes reveal dynamics of respiratory metabolism.
Barbour MM, Hanson DT., New Phytol 181(2), 2009
PMID: 19121025
On the metabolic origin of the carbon isotope composition of CO2 evolved from darkened light-acclimated leaves in Ricinus communis.
Gessler A, Tcherkez G, Karyanto O, Keitel C, Ferrio JP, Ghashghaie J, Kreuzwieser J, Farquhar GD., New Phytol 181(2), 2009
PMID: 19121034
Does photosynthesis affect grassland soil-respired CO2 and its carbon isotope composition on a diurnal timescale?
Bahn M, Schmitt M, Siegwolf R, Richter A, Brüggemann N., New Phytol 182(2), 2009
PMID: 19220762
Short-term dynamics of isotopic composition of leaf-respired CO2 upon darkening: measurements and implications.
Werner C, Wegener F, Unger S, Nogués S, Priault P., Rapid Commun Mass Spectrom 23(16), 2009
PMID: 19603472
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Kodama N, Barnard RL, Salmon Y, Weston C, Ferrio JP, Holst J, Werner RA, Saurer M, Rennenberg H, Buchmann N, Gessler A., Oecologia 156(4), 2008
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Can we use the CO2 concentrations determined by continuous-flow isotope ratio mass spectrometry from small samples for the Keeling plot approach?
Joos O, Saurer M, Heim A, Hagedorn F, Schmidt MW, Siegwolf RT., Rapid Commun Mass Spectrom 22(24), 2008
PMID: 19009520

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