Silencing of PR-10-like proteins in Medicago truncatula results in an antagonistic induction of other PR proteins and in an increased tolerance upon infection with the oomycete Aphanomyces euteiches

Colditz F, Niehaus K, Krajinski F (2007)
Planta 226(1): 57-71.

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Abstract
Recent studies on the root proteome of Medicago truncatula (Gaertn.) showed an induction of pathogenesis-related (PR) proteins of the class 10 after infection with the oomycete pathogen Aphanomyces euteiches (Drechs.). To get insights into the function of these proteins during the parasitic root-microbe association, a gene knockdown approach using RNAi was carried out. Agrobacterium rhizogenes-mediated transformation of M. truncatula roots led to a knockdown of the Medicago PR10-1 gene in transgenic in vitro root cultures. Proteomic analyses of the MtPr10-1i root cultures showed that MtPr10-1 was efficiently knocked down in two MtPr10-1i lines. Moreover, five additional PR-10-type proteins annotated as abscisic acid responsive proteins (ABR17s) revealed also an almost complete silencing in these two lines. Inoculation of the root cultures with the oomycete root pathogen A. euteiches resulted in a clearly reduced colonization and thus in a suppressed infection development in MtPr10-1i roots as compared to that in roots of the transformation controls. In addition, MtPr10-1 silencing led to the induction of a new set of PR proteins after infection with A. euteiches including the de novo induction of two isoforms of thaumatin-like proteins (PR-5b), which were not detectable in A. euteiches-infected control roots. Thus, antagonistic induction of other PR proteins, which are normally repressed due to PR-10 expression, is supposed to cause an increased resistance of M. truncatula upon an A. euteiches in vitro infection. The results were also further confirmed by detecting increased PR-5b induction levels in 2-D gels of a previously analyzed M. truncatula line (F83.005-9) exhibiting increased A. euteiches tolerance associated with reduced PR-10 induction levels.
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Colditz F, Niehaus K, Krajinski F. Silencing of PR-10-like proteins in Medicago truncatula results in an antagonistic induction of other PR proteins and in an increased tolerance upon infection with the oomycete Aphanomyces euteiches. Planta. 2007;226(1):57-71.
Colditz, F., Niehaus, K., & Krajinski, F. (2007). Silencing of PR-10-like proteins in Medicago truncatula results in an antagonistic induction of other PR proteins and in an increased tolerance upon infection with the oomycete Aphanomyces euteiches. Planta, 226(1), 57-71.
Colditz, F., Niehaus, K., and Krajinski, F. (2007). Silencing of PR-10-like proteins in Medicago truncatula results in an antagonistic induction of other PR proteins and in an increased tolerance upon infection with the oomycete Aphanomyces euteiches. Planta 226, 57-71.
Colditz, F., Niehaus, K., & Krajinski, F., 2007. Silencing of PR-10-like proteins in Medicago truncatula results in an antagonistic induction of other PR proteins and in an increased tolerance upon infection with the oomycete Aphanomyces euteiches. Planta, 226(1), p 57-71.
F. Colditz, K. Niehaus, and F. Krajinski, “Silencing of PR-10-like proteins in Medicago truncatula results in an antagonistic induction of other PR proteins and in an increased tolerance upon infection with the oomycete Aphanomyces euteiches”, Planta, vol. 226, 2007, pp. 57-71.
Colditz, F., Niehaus, K., Krajinski, F.: Silencing of PR-10-like proteins in Medicago truncatula results in an antagonistic induction of other PR proteins and in an increased tolerance upon infection with the oomycete Aphanomyces euteiches. Planta. 226, 57-71 (2007).
Colditz, Frank, Niehaus, Karsten, and Krajinski, Franziska. “Silencing of PR-10-like proteins in Medicago truncatula results in an antagonistic induction of other PR proteins and in an increased tolerance upon infection with the oomycete Aphanomyces euteiches”. Planta 226.1 (2007): 57-71.
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37 References

Data provided by Europe PubMed Central.


J-M, Grain Legumes 28(), 2000

J-M, 2001

HJ, Mol Plant Microbe Interact 6(), 1993

H, Plant Sci 165(), 2003
Characteristics of RNA silencing in plants: similarities and differences across kingdoms.
Susi P, Hohkuri M, Wahlroos T, Kilby NJ., Plant Mol. Biol. 54(2), 2004
PMID: 15159620
Several thaumatin-like proteins bind to beta-1,3-glucans.
Trudel J, Grenier J, Potvin C, Asselin A., Plant Physiol. 118(4), 1998
PMID: 9847118

LC, Plant Mol Biol Rep 12(), 1994
The PR5K receptor protein kinase from Arabidopsis thaliana is structurally related to a family of plant defense proteins.
Wang X, Zafian P, Choudhary M, Lawton M., Proc. Natl. Acad. Sci. U.S.A. 93(6), 1996
PMID: 8637920

Y, Mol Plant Microbe Interact 12(), 1999
The potential of Betv1 homologues, a nuclear multigene family, as phylogenetic markers in flowering plants.
Wen J, Vanek-Krebitz M, Hoffmann-Sommergruber K, Scheiner O, Breiteneder H., Mol. Phylogenet. Evol. 8(3), 1997
PMID: 9417891

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