Improved beta-glucanase production by a recombinant Escherichia coli strain using a zinc-ion supplemented medium

Beshay U, Miksch G, Friehs K, Flaschel E (2007)
ENGINEERING IN LIFE SCIENCES 7(3): 253-258.

Journal Article | Published | English

No fulltext has been uploaded

Abstract
In order to investigate the suitability of different metal chelates for affinity chromatography, an expression vector was constructed. It contained a hybrid beta-glucanase as a model protein fused with a His(6)-tag and a secretion cassette providing the ability to secrete beta-glucanase into the culture medium. Supplementation of zinc to the medium led to a rapidly increased expression and release of the target protein into the cultivation medium. Results in respect to the supplementation of the commonly used Terrific Broth "TB-medium" with different metal ions are reported with special emphasis on the influence of zinc ions. A concentration of zinc ions in the order of about 0.175 mM led to optimal results. Batch cultivation under well-controlled conditions showed that the growth behavior did not change significantly by adding zinc ions. Growth in a stirred tank bioreactor was much faster in unsupplemented TB-medium compared to shake flask experiments leading to a much higher biomass concentration (15 g/L instead of 3 g/L). The secretion of beta-glucanase under theses conditions started at the transition into the stationary phase and increased to yield an extracellular activity of 1350 U/mL at the end of the fermentation process. An even higher yield of extracellular beta-glucanase (2800 U/mL) was reached when the fermentation was carried out with TB-medium supplemented with 0.175 mM ZnSO4.
Publishing Year
ISSN
eISSN
PUB-ID

Cite this

Beshay U, Miksch G, Friehs K, Flaschel E. Improved beta-glucanase production by a recombinant Escherichia coli strain using a zinc-ion supplemented medium. ENGINEERING IN LIFE SCIENCES. 2007;7(3):253-258.
Beshay, U., Miksch, G., Friehs, K., & Flaschel, E. (2007). Improved beta-glucanase production by a recombinant Escherichia coli strain using a zinc-ion supplemented medium. ENGINEERING IN LIFE SCIENCES, 7(3), 253-258.
Beshay, U., Miksch, G., Friehs, K., and Flaschel, E. (2007). Improved beta-glucanase production by a recombinant Escherichia coli strain using a zinc-ion supplemented medium. ENGINEERING IN LIFE SCIENCES 7, 253-258.
Beshay, U., et al., 2007. Improved beta-glucanase production by a recombinant Escherichia coli strain using a zinc-ion supplemented medium. ENGINEERING IN LIFE SCIENCES, 7(3), p 253-258.
U. Beshay, et al., “Improved beta-glucanase production by a recombinant Escherichia coli strain using a zinc-ion supplemented medium”, ENGINEERING IN LIFE SCIENCES, vol. 7, 2007, pp. 253-258.
Beshay, U., Miksch, G., Friehs, K., Flaschel, E.: Improved beta-glucanase production by a recombinant Escherichia coli strain using a zinc-ion supplemented medium. ENGINEERING IN LIFE SCIENCES. 7, 253-258 (2007).
Beshay, U., Miksch, Gerd, Friehs, Karl, and Flaschel, Erwin. “Improved beta-glucanase production by a recombinant Escherichia coli strain using a zinc-ion supplemented medium”. ENGINEERING IN LIFE SCIENCES 7.3 (2007): 253-258.
This data publication is cited in the following publications:
This publication cites the following data publications:

Export

0 Marked Publications

Open Data PUB

Web of Science

View record in Web of Science®

Search this title in

Google Scholar