The extracytoplasmic function-type sigma factor SigM of Corynebacterium glutamicum ATCC 13032 is involved in transcription of disulfide stress-related genes

Nakunst D, Larisch C, Hueser AT, Tauch A, Pühler A, Kalinowski J (2007)
JOURNAL OF BACTERIOLOGY 189(13): 4696-4707.

Download
Es wurde kein Volltext hochgeladen. Nur Publikationsnachweis!
Zeitschriftenaufsatz | Veröffentlicht | Englisch
Autor
; ; ; ; ;
Abstract / Bemerkung
The gene for the extracytoplasmic function (ECF) sigma factor SigM was deleted from the chromosome of the gram-positive soil bacterium Corynebacterium glutamicum to elucidate the role of the SigM protein in the regulation of gene expression. Comparative DNA microarray hybridizations of the C. glutamicum wild type and sigM-deficient mutant C. glutamicum DNI revealed 23 genes with enhanced expression in the sigM-proficient strain, encoding functions in the assembly of iron-sulfur clusters (suf operon), thioredoxin reductase (trxB), thioredoxins (trxC, trxB1), chaperones (groES, groEL, clpB), and proteins involved in the heat shock response (hspR, dnaJ, grpE). Deletion of the sigM gene rendered the C. glutamicum cells more sensitive to heat, cold, and the presence of the thiol oxidant diamide. Transcription of the sigM gene increased under different stress conditions, including heat shock, cold shock, and disulfide stress caused by diamide treatment, suggesting a regulatory role for SigM under thiol-oxidative stress conditions. Stress-responsive promoters were determined upstream of the suf operon and of the trxB, trxC, and trxB1 genes. The deduced SigM consensus promoter is characterized by the - 35 hexamer gGGAAT and the - 10 hexamer YGTTGR. Transcription of the sigM gene is apparently controlled by the ECF sigma factor SigH, since a sigH mutant was unable to enhance the expression of sigM and the SigM regulon under thioloxidative stress conditions. A typical SigH-responsive promoter was mapped upstream of the sigM gene. The ECF sigma factor SigM is apparently part of a regulatory cascade, and its transcription is controlled by SigH under conditions of thiol-oxidative stress.
Erscheinungsjahr
Zeitschriftentitel
JOURNAL OF BACTERIOLOGY
Band
189
Zeitschriftennummer
13
Seite
4696-4707
ISSN
PUB-ID

Zitieren

Nakunst D, Larisch C, Hueser AT, Tauch A, Pühler A, Kalinowski J. The extracytoplasmic function-type sigma factor SigM of Corynebacterium glutamicum ATCC 13032 is involved in transcription of disulfide stress-related genes. JOURNAL OF BACTERIOLOGY. 2007;189(13):4696-4707.
Nakunst, D., Larisch, C., Hueser, A. T., Tauch, A., Pühler, A., & Kalinowski, J. (2007). The extracytoplasmic function-type sigma factor SigM of Corynebacterium glutamicum ATCC 13032 is involved in transcription of disulfide stress-related genes. JOURNAL OF BACTERIOLOGY, 189(13), 4696-4707. doi:10.1128/JB.00382-07
Nakunst, D., Larisch, C., Hueser, A. T., Tauch, A., Pühler, A., and Kalinowski, J. (2007). The extracytoplasmic function-type sigma factor SigM of Corynebacterium glutamicum ATCC 13032 is involved in transcription of disulfide stress-related genes. JOURNAL OF BACTERIOLOGY 189, 4696-4707.
Nakunst, D., et al., 2007. The extracytoplasmic function-type sigma factor SigM of Corynebacterium glutamicum ATCC 13032 is involved in transcription of disulfide stress-related genes. JOURNAL OF BACTERIOLOGY, 189(13), p 4696-4707.
D. Nakunst, et al., “The extracytoplasmic function-type sigma factor SigM of Corynebacterium glutamicum ATCC 13032 is involved in transcription of disulfide stress-related genes”, JOURNAL OF BACTERIOLOGY, vol. 189, 2007, pp. 4696-4707.
Nakunst, D., Larisch, C., Hueser, A.T., Tauch, A., Pühler, A., Kalinowski, J.: The extracytoplasmic function-type sigma factor SigM of Corynebacterium glutamicum ATCC 13032 is involved in transcription of disulfide stress-related genes. JOURNAL OF BACTERIOLOGY. 189, 4696-4707 (2007).
Nakunst, Diana, Larisch, Christof, Hueser, Andrea T., Tauch, Andreas, Pühler, Alfred, and Kalinowski, Jörn. “The extracytoplasmic function-type sigma factor SigM of Corynebacterium glutamicum ATCC 13032 is involved in transcription of disulfide stress-related genes”. JOURNAL OF BACTERIOLOGY 189.13 (2007): 4696-4707.

37 Zitationen in Europe PMC

Daten bereitgestellt von Europe PubMed Central.

Factors enhancing L-valine production by the growth-limited L-isoleucine auxotrophic strain Corynebacterium glutamicum DeltailvA DeltapanB ilvNM13 (pECKAilvBNC).
Denina I, Paegle L, Prouza M, Holátko J, Pátek M, Nesvera J, Ruklisha M., J Ind Microbiol Biotechnol 37(7), 2010
PMID: 20364396
EMMA 2--a MAGE-compliant system for the collaborative analysis and integration of microarray data.
Dondrup M, Albaum SP, Griebel T, Henckel K, Jünemann S, Kahlke T, Kleindt CK, Küster H, Linke B, Mertens D, Mittard-Runte V, Neuweger H, Runte KJ, Tauch A, Tille F, Pühler A, Goesmann A., BMC Bioinformatics 10(), 2009
PMID: 19200358
A game with many players: control of gdh transcription in Corynebacterium glutamicum.
Hänssler E, Müller T, Palumbo K, Patek M, Brocker M, Krämer R, Burkovski A., J Biotechnol 142(2), 2009
PMID: 19394370
The third pillar of bacterial signal transduction: classification of the extracytoplasmic function (ECF) sigma factor protein family.
Staroń A, Sofia HJ, Dietrich S, Ulrich LE, Liesegang H, Mascher T., Mol Microbiol 74(3), 2009
PMID: 19737356
Global gene expression mediated by Thermus thermophilus SdrP, a CRP/FNR family transcriptional regulator.
Agari Y, Kashihara A, Yokoyama S, Kuramitsu S, Shinkai A., Mol Microbiol 70(1), 2008
PMID: 18699868

69 References

Daten bereitgestellt von Europe PubMed Central.

Defining the disulphide stress response in Streptomyces coelicolor A3(2): identification of the sigmaR regulon.
Paget MS, Molle V, Cohen G, Aharonowitz Y, Buttner MJ., Mol. Microbiol. 42(4), 2001
PMID: 11737643
Promoters of Corynebacterium glutamicum.
Patek M, Nesvera J, Guyonvarch A, Reyes O, Leblon G., J. Biotechnol. 104(1-3), 2003
PMID: 12948648

AUTHOR UNKNOWN, 1989
Mycobacterium tuberculosis WhiB3 interacts with RpoV to affect host survival but is dispensable for in vivo growth.
Steyn AJ, Collins DM, Hondalus MK, Jacobs WR Jr, Kawakami RP, Bloom BR., Proc. Natl. Acad. Sci. U.S.A. 99(5), 2002
PMID: 11880648
Oxidative stress.
Storz G, Imlay JA., Curr. Opin. Microbiol. 2(2), 1999
PMID: 10322176
Corynebacterium glutamicum DNA is subjected to methylation-restriction in Escherichia coli.
Tauch A, Kirchner O, Wehmeier L, Kalinowski J, Puhler A., FEMS Microbiol. Lett. 123(3), 1994
PMID: 7988915
Efficient electrotransformation of corynebacterium diphtheriae with a mini-replicon derived from the Corynebacterium glutamicum plasmid pGA1.
Tauch A, Kirchner O, Loffler B, Gotker S, Puhler A, Kalinowski J., Curr. Microbiol. 45(5), 2002
PMID: 12232668
The CLUSTAL_X windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools.
Thompson JD, Gibson TJ, Plewniak F, Jeanmougin F, Higgins DG., Nucleic Acids Res. 25(24), 1997
PMID: 9396791
Mycothiol-dependent mycobacterial response to oxidative stress.
Ung KS, Av-Gay Y., FEBS Lett. 580(11), 2006
PMID: 16643903
How high G+C Gram-positive bacteria and in particular bifidobacteria cope with heat stress: protein players and regulators.
Ventura M, Canchaya C, Zhang Z, Bernini V, Fitzgerald GF, van Sinderen D., FEMS Microbiol. Rev. 30(5), 2006
PMID: 16911042
Cysteine desulfurase activity indicates a role for NIFS in metallocluster biosynthesis.
Zheng L, White RH, Cash VL, Jack RF, Dean DR., Proc. Natl. Acad. Sci. U.S.A. 90(7), 1993
PMID: 8464885

Export

Markieren/ Markierung löschen
Markierte Publikationen

Open Data PUB

Web of Science

Dieser Datensatz im Web of Science®

Quellen

PMID: 17483229
PubMed | Europe PMC

Suchen in

Google Scholar