Periodic acceptor excitation spectroscopy of single molecules

Doose S, Heilemann M, Michalet X, Weiss S, Kapanidis AN (2007)
EUROPEAN BIOPHYSICS JOURNAL WITH BIOPHYSICS LETTERS 36(6): 669-674.

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Abstract
Alternating-laser excitation (ALEX) spectroscopy has recently been added to the single-molecule spectroscopy toolkit. ALEX monitors interaction and stoichiometry of biomolecules, reports on biomolecular structure by measuring accurate Forster resonance energy transfer (FRET) efficiencies, and allows sorting of subpopulations on the basis of stoichiometry and FRET. Here, we demonstrate that a simple combination of one continuous-wave donor-excitation laser and one directly modulated acceptor-excitation laser (Periodic Acceptor eXcitation) is sufficient to recapitulate the capabilities of ALEX while minimizing the cost and complexity associated with use of modulation techniques.
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Doose S, Heilemann M, Michalet X, Weiss S, Kapanidis AN. Periodic acceptor excitation spectroscopy of single molecules. EUROPEAN BIOPHYSICS JOURNAL WITH BIOPHYSICS LETTERS. 2007;36(6):669-674.
Doose, S., Heilemann, M., Michalet, X., Weiss, S., & Kapanidis, A. N. (2007). Periodic acceptor excitation spectroscopy of single molecules. EUROPEAN BIOPHYSICS JOURNAL WITH BIOPHYSICS LETTERS, 36(6), 669-674.
Doose, S., Heilemann, M., Michalet, X., Weiss, S., and Kapanidis, A. N. (2007). Periodic acceptor excitation spectroscopy of single molecules. EUROPEAN BIOPHYSICS JOURNAL WITH BIOPHYSICS LETTERS 36, 669-674.
Doose, S., et al., 2007. Periodic acceptor excitation spectroscopy of single molecules. EUROPEAN BIOPHYSICS JOURNAL WITH BIOPHYSICS LETTERS, 36(6), p 669-674.
S. Doose, et al., “Periodic acceptor excitation spectroscopy of single molecules”, EUROPEAN BIOPHYSICS JOURNAL WITH BIOPHYSICS LETTERS, vol. 36, 2007, pp. 669-674.
Doose, S., Heilemann, M., Michalet, X., Weiss, S., Kapanidis, A.N.: Periodic acceptor excitation spectroscopy of single molecules. EUROPEAN BIOPHYSICS JOURNAL WITH BIOPHYSICS LETTERS. 36, 669-674 (2007).
Doose, Soren, Heilemann, Mike, Michalet, Xavier, Weiss, Shimon, and Kapanidis, Achillefs N. “Periodic acceptor excitation spectroscopy of single molecules”. EUROPEAN BIOPHYSICS JOURNAL WITH BIOPHYSICS LETTERS 36.6 (2007): 669-674.
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