Investigation of the chemical structure and biological activity of oligosaccharides isolated from rough-type Xanthomonas campestris pv. campestris B100 lipopolysaccharide

Kaczynski Z, Braun S, Lindner B, Niehaus K, Holst O (2007)
Journal of Endotoxin Research 13(2): 101-108.

Download
No fulltext has been uploaded. References only!
Journal Article | Original Article | Published | English

No fulltext has been uploaded

Author
; ; ; ;
Abstract
The rough-type lipopolysaccharide, (LPS) of the phytopathogenic bacterium Xanthomonas campestris pv. campestris B 100 was isolated utilizing the hot phenol-water method and successively de-acylated by treatment with hydrazine and hot potassium hydroxide. Four compounds were separated by preparative high-performance anion-exchange chromatography and studied by sugar analysis and by ID and 2D homonuclear and heteronuclear H-1-, C-13- and P-31-NMR spectroscopy as well as ESI FT-MS. The two main products were a heptasaccharide and a pentasaccharide of the structures (alpha-D-Manp-(1 -> 3)-alpha-D-Manp-(1 -> 4)-beta-D-Glcp-(1 -> 4)-alpha-D-Manp-3P-(1 -> 5)-alpha-Kdo-(2 -> 6)-beta-D-GlcpN-4P-(1 ->)6)-alpha-D-GlcpN-1P (1) and beta-D-Glcp-(1 -> 4)-(alpha-D-Manp-3P-(1 -> 5)-alpha-Kdo-(2-6)-alpha-D-GlcpN-4P-(1 -> 6)-alpha-D-GlcpN-1P (2), respectively. The products in smaller amounts were a heptasaccharide and pentasaccharide possessing the above structures plus a phosphate group at C-4 of the Kdo residue (compounds 3 and 4). Both, heptasaccharide 1 and pentasaccharide 2 were able to induce an oxidative burst in cell cultures of the non-host plant tobacco.
Publishing Year
ISSN
PUB-ID

Cite this

Kaczynski Z, Braun S, Lindner B, Niehaus K, Holst O. Investigation of the chemical structure and biological activity of oligosaccharides isolated from rough-type Xanthomonas campestris pv. campestris B100 lipopolysaccharide. Journal of Endotoxin Research. 2007;13(2):101-108.
Kaczynski, Z., Braun, S., Lindner, B., Niehaus, K., & Holst, O. (2007). Investigation of the chemical structure and biological activity of oligosaccharides isolated from rough-type Xanthomonas campestris pv. campestris B100 lipopolysaccharide. Journal of Endotoxin Research, 13(2), 101-108. doi:10.1177/0968051907079121
Kaczynski, Z., Braun, S., Lindner, B., Niehaus, K., and Holst, O. (2007). Investigation of the chemical structure and biological activity of oligosaccharides isolated from rough-type Xanthomonas campestris pv. campestris B100 lipopolysaccharide. Journal of Endotoxin Research 13, 101-108.
Kaczynski, Z., et al., 2007. Investigation of the chemical structure and biological activity of oligosaccharides isolated from rough-type Xanthomonas campestris pv. campestris B100 lipopolysaccharide. Journal of Endotoxin Research, 13(2), p 101-108.
Z. Kaczynski, et al., “Investigation of the chemical structure and biological activity of oligosaccharides isolated from rough-type Xanthomonas campestris pv. campestris B100 lipopolysaccharide”, Journal of Endotoxin Research, vol. 13, 2007, pp. 101-108.
Kaczynski, Z., Braun, S., Lindner, B., Niehaus, K., Holst, O.: Investigation of the chemical structure and biological activity of oligosaccharides isolated from rough-type Xanthomonas campestris pv. campestris B100 lipopolysaccharide. Journal of Endotoxin Research. 13, 101-108 (2007).
Kaczynski, Zbigniew, Braun, Sebastian, Lindner, Buko, Niehaus, Karsten, and Holst, Otto. “Investigation of the chemical structure and biological activity of oligosaccharides isolated from rough-type Xanthomonas campestris pv. campestris B100 lipopolysaccharide”. Journal of Endotoxin Research 13.2 (2007): 101-108.
This data publication is cited in the following publications:
This publication cites the following data publications:

9 Citations in Europe PMC

Data provided by Europe PubMed Central.

The influence of a modified lipopolysaccharide O-antigen on the biosynthesis of xanthan in Xanthomonas campestris pv. campestris B100.
Steffens T, Vorhölter FJ, Giampà M, Hublik G, Pühler A, Niehaus K., BMC Microbiol 16(), 2016
PMID: 27215401
Chemistry of lipid A: at the heart of innate immunity.
Molinaro A, Holst O, Di Lorenzo F, Callaghan M, Nurisso A, D'Errico G, Zamyatina A, Peri F, Berisio R, Jerala R, Jiménez-Barbero J, Silipo A, Martín-Santamaría S., Chemistry 21(2), 2015
PMID: 25353096
Characterization of a variant of Xanthomonas citri subsp. citri that triggers a host-specific defense response.
Chiesa MA, Siciliano MF, Ornella L, Roeschlin RA, Favaro MA, Delgado NP, Sendín LN, Orce IG, Ploper LD, Vojnov AA, Vacas JG, Filippone MP, Castagnaro AP, Marano MR., Phytopathology 103(6), 2013
PMID: 23268580
Dynamic protein phosphorylation during the growth of Xanthomonas campestris pv. campestris B100 revealed by a gel-based proteomics approach.
Musa YR, Bäsell K, Schatschneider S, Vorhölter FJ, Becher D, Niehaus K., J Biotechnol 167(2), 2013
PMID: 23792782
Glyco-conjugates as elicitors or suppressors of plant innate immunity.
Silipo A, Erbs G, Shinya T, Dow JM, Parrilli M, Lanzetta R, Shibuya N, Newman MA, Molinaro A., Glycobiology 20(4), 2010
PMID: 20018942
The structure of the O-specific polysaccharide of the lipopolysaccharide from Pantoea agglomerans strain FL1.
Cimmino A, Marchi G, Surico G, Hanuszkiewicz A, Evidente A, Holst O., Carbohydr Res 343(2), 2008
PMID: 18039532

Export

0 Marked Publications

Open Data PUB

Web of Science

View record in Web of Science®

Sources

PMID: 17621551
PubMed | Europe PMC

Search this title in

Google Scholar