Involvement of a gelsolin-related protein in spermatogenesis of the earthworm Lumbricus terrestris

Krueger E, Hinssen H, D'Haese J (2008)
CELL AND TISSUE RESEARCH 332(1): 141-150.

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Abstract
A gelsolin-related protein was isolated from seminal vesicles of the annelid Lumbricus terrestris. Compared with the isoforms of the gelsolin-related protein previously found in the muscle of the annelid body wall, the isolated protein was assigned to the first isoform (EWAM-P1) because of its electrophoretic mobility, chromatographic elution behaviour, immunological cross-reactivity and identical nucleotide sequence of segments obtained by reverse transcription/polymerase chain reaction. Immunofluorescence studies with smear preparations of developing male germ cells revealed characteristic changes of the local distribution of actin and EWAM-P1 during spermatogenesis. These changes were correlated with the developmental transport processes and structural alterations. F-actin, as revealed by rhodamine-phalloidin staining, formed a toroid-shaped structure in cytoplasmic bridges connecting the germ cells to a central cytophore during the developmental stages. An actin antibody reacting with both G- and F-actin demonstrated that actin was concentrated at the proximal and distal parts of the spermatocytes. EWAM-P1 was also localized in these regions, with intense staining in the distal part of spermatocytes and young spermatids in which the Golgi complex and proacrosome resided. The anti-actin antibody further stained the periphery of the nucleus. This staining gradually reduced during sperm maturation and covered about half of the length of the nucleus in elongated spermatids. Co-localization of EWAM with actin implied a functional significance of this gelsolin-related protein for the rearrangement of the actin cytoskeleton during earthworm spermiogenesis.
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Krueger E, Hinssen H, D'Haese J. Involvement of a gelsolin-related protein in spermatogenesis of the earthworm Lumbricus terrestris. CELL AND TISSUE RESEARCH. 2008;332(1):141-150.
Krueger, E., Hinssen, H., & D'Haese, J. (2008). Involvement of a gelsolin-related protein in spermatogenesis of the earthworm Lumbricus terrestris. CELL AND TISSUE RESEARCH, 332(1), 141-150.
Krueger, E., Hinssen, H., and D'Haese, J. (2008). Involvement of a gelsolin-related protein in spermatogenesis of the earthworm Lumbricus terrestris. CELL AND TISSUE RESEARCH 332, 141-150.
Krueger, E., Hinssen, H., & D'Haese, J., 2008. Involvement of a gelsolin-related protein in spermatogenesis of the earthworm Lumbricus terrestris. CELL AND TISSUE RESEARCH, 332(1), p 141-150.
E. Krueger, H. Hinssen, and J. D'Haese, “Involvement of a gelsolin-related protein in spermatogenesis of the earthworm Lumbricus terrestris”, CELL AND TISSUE RESEARCH, vol. 332, 2008, pp. 141-150.
Krueger, E., Hinssen, H., D'Haese, J.: Involvement of a gelsolin-related protein in spermatogenesis of the earthworm Lumbricus terrestris. CELL AND TISSUE RESEARCH. 332, 141-150 (2008).
Krueger, Evelyn, Hinssen, Horst, and D'Haese, Jochen. “Involvement of a gelsolin-related protein in spermatogenesis of the earthworm Lumbricus terrestris”. CELL AND TISSUE RESEARCH 332.1 (2008): 141-150.
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