Involvement of a gelsolin-related protein in spermatogenesis of the earthworm Lumbricus terrestris

Krueger E, Hinssen H, D'Haese J (2008)
CELL AND TISSUE RESEARCH 332(1): 141-150.

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Zeitschriftenaufsatz | Veröffentlicht | Englisch
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Abstract / Bemerkung
A gelsolin-related protein was isolated from seminal vesicles of the annelid Lumbricus terrestris. Compared with the isoforms of the gelsolin-related protein previously found in the muscle of the annelid body wall, the isolated protein was assigned to the first isoform (EWAM-P1) because of its electrophoretic mobility, chromatographic elution behaviour, immunological cross-reactivity and identical nucleotide sequence of segments obtained by reverse transcription/polymerase chain reaction. Immunofluorescence studies with smear preparations of developing male germ cells revealed characteristic changes of the local distribution of actin and EWAM-P1 during spermatogenesis. These changes were correlated with the developmental transport processes and structural alterations. F-actin, as revealed by rhodamine-phalloidin staining, formed a toroid-shaped structure in cytoplasmic bridges connecting the germ cells to a central cytophore during the developmental stages. An actin antibody reacting with both G- and F-actin demonstrated that actin was concentrated at the proximal and distal parts of the spermatocytes. EWAM-P1 was also localized in these regions, with intense staining in the distal part of spermatocytes and young spermatids in which the Golgi complex and proacrosome resided. The anti-actin antibody further stained the periphery of the nucleus. This staining gradually reduced during sperm maturation and covered about half of the length of the nucleus in elongated spermatids. Co-localization of EWAM with actin implied a functional significance of this gelsolin-related protein for the rearrangement of the actin cytoskeleton during earthworm spermiogenesis.
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Zeitschriftentitel
CELL AND TISSUE RESEARCH
Band
332
Zeitschriftennummer
1
Seite
141-150
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Krueger E, Hinssen H, D'Haese J. Involvement of a gelsolin-related protein in spermatogenesis of the earthworm Lumbricus terrestris. CELL AND TISSUE RESEARCH. 2008;332(1):141-150.
Krueger, E., Hinssen, H., & D'Haese, J. (2008). Involvement of a gelsolin-related protein in spermatogenesis of the earthworm Lumbricus terrestris. CELL AND TISSUE RESEARCH, 332(1), 141-150. doi:10.1007/s00441-007-0561-9
Krueger, E., Hinssen, H., and D'Haese, J. (2008). Involvement of a gelsolin-related protein in spermatogenesis of the earthworm Lumbricus terrestris. CELL AND TISSUE RESEARCH 332, 141-150.
Krueger, E., Hinssen, H., & D'Haese, J., 2008. Involvement of a gelsolin-related protein in spermatogenesis of the earthworm Lumbricus terrestris. CELL AND TISSUE RESEARCH, 332(1), p 141-150.
E. Krueger, H. Hinssen, and J. D'Haese, “Involvement of a gelsolin-related protein in spermatogenesis of the earthworm Lumbricus terrestris”, CELL AND TISSUE RESEARCH, vol. 332, 2008, pp. 141-150.
Krueger, E., Hinssen, H., D'Haese, J.: Involvement of a gelsolin-related protein in spermatogenesis of the earthworm Lumbricus terrestris. CELL AND TISSUE RESEARCH. 332, 141-150 (2008).
Krueger, Evelyn, Hinssen, Horst, and D'Haese, Jochen. “Involvement of a gelsolin-related protein in spermatogenesis of the earthworm Lumbricus terrestris”. CELL AND TISSUE RESEARCH 332.1 (2008): 141-150.

7 Zitationen in Europe PMC

Daten bereitgestellt von Europe PubMed Central.

Four paralog gelsolin genes are differentially expressed in the earthworm Lumbricus terrestris.
Thiruketheeswaran P, Thomalla P, Krüger E, Hinssen H, D'Haese J., Comp Biochem Physiol B Biochem Mol Biol 208-209(), 2017
PMID: 28400331
Unique phenotypes in the sperm of the earthworm Eudrilus eugeniae for assessing radiation hazards.
Yesudhason BV, Jegathambigai J, Thangasamy PA, Lakshmanan DD, Selvan Christyraj JR, Sathya Balasingh Thangapandi EJ, Krishnan M, Sivasubramaniam S., Environ Monit Assess 185(6), 2013
PMID: 23093367
Isoforms of gelsolin from lobster striated muscles differ in calcium-dependence.
Unger A, Brunne B, Hinssen H., Arch Biochem Biophys 536(1), 2013
PMID: 23707758

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