Glycosphingolipids in vascular endothelial cells: relationship of heterogeneity in Gb3Cer/CD77 receptor expression with differential Shiga toxin 1 cytotoxicity

Schweppe CH, Bielaszewska M, Pohlentz G, Friedrich AW, Büntemeyer H, Schmidt MA, Kim KS, Peter-Katalinic J, Karch H, Müthing J (2008)
GLYCOCONJUGATE JOURNAL 25(4): 291-304.

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Abstract
Shiga toxin (Stx) 1 binds to the glycosphingolipid (GSL) globotriaosylceramide (Gb3Cer/CD77) and injures human endothelial cells. In order to gain insight into Stx1-induced cellular impairment, we analysed in detail the molecular heterogeneity of Stx1 receptors in two endothelial cell lines differing in their Stx1-sensitivity. We observed a moderate sensitivity to Stx1 of human brain microvascular endothelial cells (HBMECs, CD50 > 200 ng/ml), but a considerably higher mortality rate in cultures of EA.hy 926 cells, a cell line derived from human umbilical vein endothelial cells (CD50 of 0.2 ng/ml). Immunofluorescence microscopy demonstrated the presence of Gb3Cer in both cell lines, but showed an enhanced content of Gb3Cer in EA.hy 926 cells. Solid phase overlay binding assays of isolated GSLs combined with nanoelectrospray ionization quadrupole time-of-flight mass spectrometry demonstrated a balanced proportion of Gb3Cer and globotetraosylceramide (Gb4Cer) in HBMECs, but an increase of Gb3Cer and absence of Gb4Cer in EA.hy 926 cells. Gb3Cer species with C24:1/C24:0 fatty acids were found to dominate over those with C16:0 fatty acids in EA.hy 926 cells, but were similarly distributed in HBMECs. Reverse transcriptase polymerase chain reaction indicated the concomitant presence of Gb3Cer and Gb4Cer synthases in HBMECs, whereas EA.hy 926 cells expressed Gb3Cer synthase, but completely lacked Gb4Cer synthase. This deficiency, resulting in the accumulation of Gb3Cer in EA.hy 926 cells, represents the most prominent molecular reason that underlies the different Stx1 sensitivities of HBMECs and EA.hy 926 endothelial cells.
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Schweppe CH, Bielaszewska M, Pohlentz G, et al. Glycosphingolipids in vascular endothelial cells: relationship of heterogeneity in Gb3Cer/CD77 receptor expression with differential Shiga toxin 1 cytotoxicity. GLYCOCONJUGATE JOURNAL. 2008;25(4):291-304.
Schweppe, C. H., Bielaszewska, M., Pohlentz, G., Friedrich, A. W., Büntemeyer, H., Schmidt, M. A., Kim, K. S., et al. (2008). Glycosphingolipids in vascular endothelial cells: relationship of heterogeneity in Gb3Cer/CD77 receptor expression with differential Shiga toxin 1 cytotoxicity. GLYCOCONJUGATE JOURNAL, 25(4), 291-304.
Schweppe, C. H., Bielaszewska, M., Pohlentz, G., Friedrich, A. W., Büntemeyer, H., Schmidt, M. A., Kim, K. S., Peter-Katalinic, J., Karch, H., and Müthing, J. (2008). Glycosphingolipids in vascular endothelial cells: relationship of heterogeneity in Gb3Cer/CD77 receptor expression with differential Shiga toxin 1 cytotoxicity. GLYCOCONJUGATE JOURNAL 25, 291-304.
Schweppe, C.H., et al., 2008. Glycosphingolipids in vascular endothelial cells: relationship of heterogeneity in Gb3Cer/CD77 receptor expression with differential Shiga toxin 1 cytotoxicity. GLYCOCONJUGATE JOURNAL, 25(4), p 291-304.
C.H. Schweppe, et al., “Glycosphingolipids in vascular endothelial cells: relationship of heterogeneity in Gb3Cer/CD77 receptor expression with differential Shiga toxin 1 cytotoxicity”, GLYCOCONJUGATE JOURNAL, vol. 25, 2008, pp. 291-304.
Schweppe, C.H., Bielaszewska, M., Pohlentz, G., Friedrich, A.W., Büntemeyer, H., Schmidt, M.A., Kim, K.S., Peter-Katalinic, J., Karch, H., Müthing, J.: Glycosphingolipids in vascular endothelial cells: relationship of heterogeneity in Gb3Cer/CD77 receptor expression with differential Shiga toxin 1 cytotoxicity. GLYCOCONJUGATE JOURNAL. 25, 291-304 (2008).
Schweppe, Christian H., Bielaszewska, Martina, Pohlentz, Gottfried, Friedrich, Alexander W., Büntemeyer, Heino, Schmidt, M. Alexander, Kim, Kwang S., Peter-Katalinic, Jasna, Karch, Helge, and Müthing, Johannes. “Glycosphingolipids in vascular endothelial cells: relationship of heterogeneity in Gb3Cer/CD77 receptor expression with differential Shiga toxin 1 cytotoxicity”. GLYCOCONJUGATE JOURNAL 25.4 (2008): 291-304.
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PMID: 25819809
Glycoconjugates and related molecules in human vascular endothelial cells.
Sasaki N, Toyoda M., Int J Vasc Med 2013(), 2013
PMID: 24171112
Expression of Shiga toxin 2e glycosphingolipid receptors of primary porcine brain endothelial cells and toxin-mediated breakdown of the blood-brain barrier.
Meisen I, Rosenbruck R, Galla HJ, Huwel S, Kouzel IU, Mormann M, Karch H, Muthing J., Glycobiology 23(6), 2013
PMID: 23431059
Association of Shiga toxin glycosphingolipid receptors with membrane microdomains of toxin-sensitive lymphoid and myeloid cells.
Kouzel IU, Pohlentz G, Storck W, Radamm L, Hoffmann P, Bielaszewska M, Bauwens A, Cichon C, Schmidt MA, Mormann M, Karch H, Muthing J., J. Lipid Res. 54(3), 2013
PMID: 23248329
Hypercalcemia induces a proinflammatory phenotype in rat leukocytes and endothelial cells.
Rezic-Muzinic N, Cikes-Culic V, Bozic J, Ticinovic-Kurir T, Salamunic I, Markotic A., J. Physiol. Biochem. 69(2), 2013
PMID: 23011779
Facing glycosphingolipid-Shiga toxin interaction: dire straits for endothelial cells of the human vasculature.
Bauwens A, Betz J, Meisen I, Kemper B, Karch H, Muthing J., Cell. Mol. Life Sci. 70(3), 2013
PMID: 22766973
Glycosphingolipid profile of the apical pole of human placental capillaries: the relevancy of the observed data to Fabry disease.
Hulkova H, Ledvinova J, Kuchar L, Smid F, Honzikova J, Elleder M., Glycobiology 22(5), 2012
PMID: 22319058
Promiscuous Shiga toxin 2e and its intimate relationship to Forssman.
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PMID: 22279060
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Betz J, Bielaszewska M, Thies A, Humpf HU, Dreisewerd K, Karch H, Kim KS, Friedrich AW, Muthing J., J. Lipid Res. 52(4), 2011
PMID: 21252262
On the structural diversity of Shiga toxin glycosphingolipid receptors in lymphoid and myeloid cells determined by nanoelectrospray ionization tandem mass spectrometry.
Hoffmann P, Hulsewig M, Duvar S, Ziehr H, Mormann M, Peter-Katalinic J, Friedrich AW, Karch H, Muthing J., Rapid Commun. Mass Spectrom. 24(15), 2010
PMID: 20623712
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Mass spectrometry of fluorocarbon-labeled glycosphingolipids.
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PMID: 20301184
Application of thin-layer chromatography/infrared matrix-assisted laser desorption/ionization orthogonal time-of-flight mass spectrometry to structural analysis of bacteria-binding glycosphingolipids selected by affinity detection.
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PMID: 20213676
Induction of apoptosis by Shiga toxins.
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PMID: 20210553
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Distler U, Souady J, Hulsewig M, Drmic-Hofman I, Haier J, Friedrich AW, Karch H, Senninger N, Dreisewerd K, Berkenkamp S, Schmidt MA, Peter-Katalinic J, Muthing J., PLoS ONE 4(8), 2009
PMID: 19714252
Fabry disease.
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PMID: 19318041

75 References

Data provided by Europe PubMed Central.


Domon, Glycoconj. J. 5(), 1988
Endothelial heterogeneity in Shiga toxin receptors and responses.
Obrig TG, Louise CB, Lingwood CA, Boyd B, Barley-Maloney L, Daniel TO., J. Biol. Chem. 268(21), 1993
PMID: 8340376
Human microvascular endothelial cells are strongly sensitive to Shiga toxins.
Ohmi K, Kiyokawa N, Takeda T, Fujimoto J., Biochem. Biophys. Res. Commun. 251(1), 1998
PMID: 9790920
Responses of human intestinal microvascular endothelial cells to Shiga toxins 1 and 2 and pathogenesis of hemorrhagic colitis.
Jacewicz MS, Acheson DW, Binion DG, West GA, Lincicome LL, Fiocchi C, Keusch GT., Infect. Immun. 67(3), 1999
PMID: 10024592
Effect of globotriaosyl ceramide fatty acid alpha-hydroxylation on the binding by verotoxin 1 and verotoxin 2.
Binnington B, Lingwood D, Nutikka A, Lingwood CA., Neurochem. Res. 27(7-8), 2002
PMID: 12374217
Characterization of a shiga-toxin 1-resistant stock of vero cells.
Sekino T, Kiyokawa N, Taguchi T, Takenouchi H, Matsui J, Tang WR, Suzuki T, Nakajima H, Saito M, Ohmi K, Katagiri YU, Okita H, Nakao H, Takeda T, Fujimoto J., Microbiol. Immunol. 48(5), 2004
PMID: 15215625
Dynamic and structural properties of sphingolipids as driving forces for the formation of membrane domains.
Sonnino S, Prinetti A, Mauri L, Chigorno V, Tettamanti G., Chem. Rev. 106(6), 2006
PMID: 16771445
Raft trafficking of AB5 subunit bacterial toxins.
Lencer WI, Saslowsky D., Biochim. Biophys. Acta 1746(3), 2005
PMID: 16153723
Targeting of Shiga toxin B-subunit to retrograde transport route in association with detergent-resistant membranes.
Falguieres T, Mallard F, Baron C, Hanau D, Lingwood C, Goud B, Salamero J, Johannes L., Mol. Biol. Cell 12(8), 2001
PMID: 11514628
Functionally different pools of Shiga toxin receptor, globotriaosyl ceramide, in HeLa cells.
Falguieres T, Romer W, Amessou M, Afonso C, Wolf C, Tabet JC, Lamaze C, Johannes L., FEBS J. 273(22), 2006
PMID: 17059464
Shiga-toxin-producing Escherichia coli and haemolytic uraemic syndrome.
Tarr PI, Gordon CA, Chandler WL., Lancet 365(9464), 2005
PMID: 15781103
An improved semi-quantitative enzyme immunostaining procedure for glycosphingolipid antigens on high performance thin layer chromatograms.
Bethke U, Muthing J, Schauder B, Conradt P, Muhlradt PF., J. Immunol. Methods 89(1), 1986
PMID: 3517170
Oxygenation alters ganglioside expression in rat liver following partial hepatectomy.
Markotic A, Culic VC, Kurir TT, Meisen I, Buntemeyer H, Boraska V, Zemunik T, Petri N, Mesaric M, Peter-Katalinic J, Muthing J., Biochem. Biophys. Res. Commun. 330(1), 2005
PMID: 15781242

Chester, Glycoconj. J. 16(), 1999

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