Glycosphingolipids in vascular endothelial cells: relationship of heterogeneity in Gb3Cer/CD77 receptor expression with differential Shiga toxin 1 cytotoxicity

Schweppe CH, Bielaszewska M, Pohlentz G, Friedrich AW, Büntemeyer H, Schmidt MA, Kim KS, Peter-Katalinic J, Karch H, Müthing J (2008)
GLYCOCONJUGATE JOURNAL 25(4): 291-304.

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Zeitschriftenaufsatz | Veröffentlicht | Englisch
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Abstract / Bemerkung
Shiga toxin (Stx) 1 binds to the glycosphingolipid (GSL) globotriaosylceramide (Gb3Cer/CD77) and injures human endothelial cells. In order to gain insight into Stx1-induced cellular impairment, we analysed in detail the molecular heterogeneity of Stx1 receptors in two endothelial cell lines differing in their Stx1-sensitivity. We observed a moderate sensitivity to Stx1 of human brain microvascular endothelial cells (HBMECs, CD50 > 200 ng/ml), but a considerably higher mortality rate in cultures of EA.hy 926 cells, a cell line derived from human umbilical vein endothelial cells (CD50 of 0.2 ng/ml). Immunofluorescence microscopy demonstrated the presence of Gb3Cer in both cell lines, but showed an enhanced content of Gb3Cer in EA.hy 926 cells. Solid phase overlay binding assays of isolated GSLs combined with nanoelectrospray ionization quadrupole time-of-flight mass spectrometry demonstrated a balanced proportion of Gb3Cer and globotetraosylceramide (Gb4Cer) in HBMECs, but an increase of Gb3Cer and absence of Gb4Cer in EA.hy 926 cells. Gb3Cer species with C24:1/C24:0 fatty acids were found to dominate over those with C16:0 fatty acids in EA.hy 926 cells, but were similarly distributed in HBMECs. Reverse transcriptase polymerase chain reaction indicated the concomitant presence of Gb3Cer and Gb4Cer synthases in HBMECs, whereas EA.hy 926 cells expressed Gb3Cer synthase, but completely lacked Gb4Cer synthase. This deficiency, resulting in the accumulation of Gb3Cer in EA.hy 926 cells, represents the most prominent molecular reason that underlies the different Stx1 sensitivities of HBMECs and EA.hy 926 endothelial cells.
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Zeitschriftentitel
GLYCOCONJUGATE JOURNAL
Band
25
Zeitschriftennummer
4
Seite
291-304
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eISSN
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Schweppe CH, Bielaszewska M, Pohlentz G, et al. Glycosphingolipids in vascular endothelial cells: relationship of heterogeneity in Gb3Cer/CD77 receptor expression with differential Shiga toxin 1 cytotoxicity. GLYCOCONJUGATE JOURNAL. 2008;25(4):291-304.
Schweppe, C. H., Bielaszewska, M., Pohlentz, G., Friedrich, A. W., Büntemeyer, H., Schmidt, M. A., Kim, K. S., et al. (2008). Glycosphingolipids in vascular endothelial cells: relationship of heterogeneity in Gb3Cer/CD77 receptor expression with differential Shiga toxin 1 cytotoxicity. GLYCOCONJUGATE JOURNAL, 25(4), 291-304. doi:10.1007/s10719-007-9091-7
Schweppe, C. H., Bielaszewska, M., Pohlentz, G., Friedrich, A. W., Büntemeyer, H., Schmidt, M. A., Kim, K. S., Peter-Katalinic, J., Karch, H., and Müthing, J. (2008). Glycosphingolipids in vascular endothelial cells: relationship of heterogeneity in Gb3Cer/CD77 receptor expression with differential Shiga toxin 1 cytotoxicity. GLYCOCONJUGATE JOURNAL 25, 291-304.
Schweppe, C.H., et al., 2008. Glycosphingolipids in vascular endothelial cells: relationship of heterogeneity in Gb3Cer/CD77 receptor expression with differential Shiga toxin 1 cytotoxicity. GLYCOCONJUGATE JOURNAL, 25(4), p 291-304.
C.H. Schweppe, et al., “Glycosphingolipids in vascular endothelial cells: relationship of heterogeneity in Gb3Cer/CD77 receptor expression with differential Shiga toxin 1 cytotoxicity”, GLYCOCONJUGATE JOURNAL, vol. 25, 2008, pp. 291-304.
Schweppe, C.H., Bielaszewska, M., Pohlentz, G., Friedrich, A.W., Büntemeyer, H., Schmidt, M.A., Kim, K.S., Peter-Katalinic, J., Karch, H., Müthing, J.: Glycosphingolipids in vascular endothelial cells: relationship of heterogeneity in Gb3Cer/CD77 receptor expression with differential Shiga toxin 1 cytotoxicity. GLYCOCONJUGATE JOURNAL. 25, 291-304 (2008).
Schweppe, Christian H., Bielaszewska, Martina, Pohlentz, Gottfried, Friedrich, Alexander W., Büntemeyer, Heino, Schmidt, M. Alexander, Kim, Kwang S., Peter-Katalinic, Jasna, Karch, Helge, and Müthing, Johannes. “Glycosphingolipids in vascular endothelial cells: relationship of heterogeneity in Gb3Cer/CD77 receptor expression with differential Shiga toxin 1 cytotoxicity”. GLYCOCONJUGATE JOURNAL 25.4 (2008): 291-304.

28 Zitationen in Europe PMC

Daten bereitgestellt von Europe PubMed Central.

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PMID: 20210553
Fabry disease.
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PMID: 19318041
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PMID: 19714252

75 References

Daten bereitgestellt von Europe PubMed Central.


Domon, Glycoconj. J. 5(), 1988
Endothelial heterogeneity in Shiga toxin receptors and responses.
Obrig TG, Louise CB, Lingwood CA, Boyd B, Barley-Maloney L, Daniel TO., J. Biol. Chem. 268(21), 1993
PMID: 8340376
Human microvascular endothelial cells are strongly sensitive to Shiga toxins.
Ohmi K, Kiyokawa N, Takeda T, Fujimoto J., Biochem. Biophys. Res. Commun. 251(1), 1998
PMID: 9790920
Responses of human intestinal microvascular endothelial cells to Shiga toxins 1 and 2 and pathogenesis of hemorrhagic colitis.
Jacewicz MS, Acheson DW, Binion DG, West GA, Lincicome LL, Fiocchi C, Keusch GT., Infect. Immun. 67(3), 1999
PMID: 10024592
Effect of globotriaosyl ceramide fatty acid alpha-hydroxylation on the binding by verotoxin 1 and verotoxin 2.
Binnington B, Lingwood D, Nutikka A, Lingwood CA., Neurochem. Res. 27(7-8), 2002
PMID: 12374217
Characterization of a shiga-toxin 1-resistant stock of vero cells.
Sekino T, Kiyokawa N, Taguchi T, Takenouchi H, Matsui J, Tang WR, Suzuki T, Nakajima H, Saito M, Ohmi K, Katagiri YU, Okita H, Nakao H, Takeda T, Fujimoto J., Microbiol. Immunol. 48(5), 2004
PMID: 15215625
Dynamic and structural properties of sphingolipids as driving forces for the formation of membrane domains.
Sonnino S, Prinetti A, Mauri L, Chigorno V, Tettamanti G., Chem. Rev. 106(6), 2006
PMID: 16771445
Raft trafficking of AB5 subunit bacterial toxins.
Lencer WI, Saslowsky D., Biochim. Biophys. Acta 1746(3), 2005
PMID: 16153723
Targeting of Shiga toxin B-subunit to retrograde transport route in association with detergent-resistant membranes.
Falguieres T, Mallard F, Baron C, Hanau D, Lingwood C, Goud B, Salamero J, Johannes L., Mol. Biol. Cell 12(8), 2001
PMID: 11514628
Functionally different pools of Shiga toxin receptor, globotriaosyl ceramide, in HeLa cells.
Falguieres T, Romer W, Amessou M, Afonso C, Wolf C, Tabet JC, Lamaze C, Johannes L., FEBS J. 273(22), 2006
PMID: 17059464
Shiga-toxin-producing Escherichia coli and haemolytic uraemic syndrome.
Tarr PI, Gordon CA, Chandler WL., Lancet 365(9464), 2005
PMID: 15781103
An improved semi-quantitative enzyme immunostaining procedure for glycosphingolipid antigens on high performance thin layer chromatograms.
Bethke U, Muthing J, Schauder B, Conradt P, Muhlradt PF., J. Immunol. Methods 89(1), 1986
PMID: 3517170
Oxygenation alters ganglioside expression in rat liver following partial hepatectomy.
Markotic A, Culic VC, Kurir TT, Meisen I, Buntemeyer H, Boraska V, Zemunik T, Petri N, Mesaric M, Peter-Katalinic J, Muthing J., Biochem. Biophys. Res. Commun. 330(1), 2005
PMID: 15781242

Chester, Glycoconj. J. 16(), 1999

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