Enhanced polarizing microscopy as a new tool in aneuploidy research in oocytes

Shen Y, Betzendahl I, Tinneberg H-R, Eichenlaub-Ritter U (2008)
MUTATION RESEARCH-GENETIC TOXICOLOGY AND ENVIRONMENTAL MUTAGENESIS 651(1-2): 131-140.

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Abstract
Chromosomal non-disjunction in female meiosis gives rise to reduced fertility and trisomy in humans. Human oocytes, especially from aged women, appear especially susceptible to non-disjunction. The oocyte spindle is crucial for high fidelity of chromosome segregation at meiotic divisions, and alterations in spindle morphology are therefore indicators of adverse conditions during oocyte development that may result in meiotic aneuploidy. In the past, oocytes had to be fixed for spindle analysis, precluding direct non-invasive identification of aneugens and adverse maturation conditions that affect spindle integrity and chromosome behaviour. Aneuploidy research for detection of spindle aberrations was therefore mainly focused on in vivo or in vitro exposed, fixed animal oocytes or cytogenetic analysis of spread oocytes. Orientation independent enhanced polarizing microscopy with nearly circularly polarized light and electronically controlled liquid crystal compensator optics is a new tool to study spindle morphology non-invasively in vivo for qualitative as well as quantitative analysis. Image generation by polarization microscopy depends on the intrinsic optical properties of the spindle with its paracrystalline microtubule lattice. When polarized light passes through such a lattice it induces a splitting of the beam and shift in the plane of vibration and retardation of light (termed birefringence and retardance). Studies of animal oocytes and follicle-cell denuded human oocytes fertilized by intracytoplasmic sperm injection for assisted conception have demonstrated the safety and efficacy of enhanced polarization microscopy. The method can be employed in aneuploidy research for non-invasive dose-response studies to detect spindle aberrations, for instance, in combination with cytogenetic analysis. Due to the non-invasive nature of the technique it may be employed in routine analysis of human oocytes to assess risks by lifestyle factors, and occupational and adverse environmental exposures. (c) 2007 Elsevier B.V. All rights reserved.
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Shen Y, Betzendahl I, Tinneberg H-R, Eichenlaub-Ritter U. Enhanced polarizing microscopy as a new tool in aneuploidy research in oocytes. MUTATION RESEARCH-GENETIC TOXICOLOGY AND ENVIRONMENTAL MUTAGENESIS. 2008;651(1-2):131-140.
Shen, Y., Betzendahl, I., Tinneberg, H. - R., & Eichenlaub-Ritter, U. (2008). Enhanced polarizing microscopy as a new tool in aneuploidy research in oocytes. MUTATION RESEARCH-GENETIC TOXICOLOGY AND ENVIRONMENTAL MUTAGENESIS, 651(1-2), 131-140.
Shen, Y., Betzendahl, I., Tinneberg, H. - R., and Eichenlaub-Ritter, U. (2008). Enhanced polarizing microscopy as a new tool in aneuploidy research in oocytes. MUTATION RESEARCH-GENETIC TOXICOLOGY AND ENVIRONMENTAL MUTAGENESIS 651, 131-140.
Shen, Y., et al., 2008. Enhanced polarizing microscopy as a new tool in aneuploidy research in oocytes. MUTATION RESEARCH-GENETIC TOXICOLOGY AND ENVIRONMENTAL MUTAGENESIS, 651(1-2), p 131-140.
Y. Shen, et al., “Enhanced polarizing microscopy as a new tool in aneuploidy research in oocytes”, MUTATION RESEARCH-GENETIC TOXICOLOGY AND ENVIRONMENTAL MUTAGENESIS, vol. 651, 2008, pp. 131-140.
Shen, Y., Betzendahl, I., Tinneberg, H.-R., Eichenlaub-Ritter, U.: Enhanced polarizing microscopy as a new tool in aneuploidy research in oocytes. MUTATION RESEARCH-GENETIC TOXICOLOGY AND ENVIRONMENTAL MUTAGENESIS. 651, 131-140 (2008).
Shen, Ying, Betzendahl, Ilse, Tinneberg, Hans-Rudolf, and Eichenlaub-Ritter, Ursula. “Enhanced polarizing microscopy as a new tool in aneuploidy research in oocytes”. MUTATION RESEARCH-GENETIC TOXICOLOGY AND ENVIRONMENTAL MUTAGENESIS 651.1-2 (2008): 131-140.
This data publication is cited in the following publications:
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Data provided by Europe PubMed Central.

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Mohammadi-Sangcheshmeh A, Held E, Rings F, Ghanem N, Salilew-Wondim D, Tesfaye D, Sieme H, Schellander K, Hoelker M., Reprod. Fertil. Dev. 26(3), 2014
PMID: 23622680
Quality control of embryo development.
Ajduk A, Zernicka-Goetz M., Mol. Aspects Med. 34(5), 2013
PMID: 23563243
Assessment of meiotic spindle configuration and post-warming bovine oocyte viability using polarized light microscopy.
Caamano JN, Diez C, Trigal B, Munoz M, Morato R, Martin D, Carrocera S, Mogas T, Gomez E., Reprod. Domest. Anim. 48(3), 2013
PMID: 23106568
Zona pellucida birefringence correlates with developmental capacity of bovine oocytes classified by maturational environment, COC morphology and G6PDH activity.
Held E, Mertens EM, Mohammadi-Sangcheshmeh A, Salilew-Wondim D, Besenfelder U, Havlicek V, Herrler A, Tesfaye D, Schellander K, Holker M., Reprod. Fertil. Dev. 24(4), 2012
PMID: 22541545
Evidence that carbonyl stress by methylglyoxal exposure induces DNA damage and spindle aberrations, affects mitochondrial integrity in mammalian oocytes and contributes to oocyte ageing.
Tatone C, Heizenrieder T, Di Emidio G, Treffon P, Amicarelli F, Seidel T, Eichenlaub-Ritter U., Hum. Reprod. 26(7), 2011
PMID: 21558076
Gamete competence assessment by polarizing optics in assisted reproduction.
Montag M, Koster M, van der Ven K, van der Ven H., Hum. Reprod. Update 17(5), 2011
PMID: 21531752
Polarized light microscopy in mammalian oocytes.
Caamano JN, Munoz M, Diez C, Gomez E., Reprod. Domest. Anim. 45 Suppl 2(), 2010
PMID: 20591065
MCAK is present at centromeres, midspindle and chiasmata and involved in silencing of the spindle assembly checkpoint in mammalian oocytes.
Vogt E, Sanhaji M, Klein W, Seidel T, Wordeman L, Eichenlaub-Ritter U., Mol. Hum. Reprod. 16(9), 2010
PMID: 20406800
Comparative analysis of the metaphase II spindle of human oocytes through polarized light and high-performance confocal microscopy.
Coticchio G, Sciajno R, Hutt K, Bromfield J, Borini A, Albertini DF., Fertil. Steril. 93(6), 2010
PMID: 19243751

99 References

Data provided by Europe PubMed Central.

Quantifying single and bundled microtubules with the polarized light microscope.
Tran P, Salmon ED, Oldenbourg R., Biol. Bull. 189(2), 1995
PMID: 8541402
High magnitude of light retardation by the zona pellucida is associated with conception cycles.
Shen Y, Stalf T, Mehnert C, Eichenlaub-Ritter U, Tinneberg HR., Hum. Reprod. 20(6), 2005
PMID: 15734754
Light retardance by human oocyte spindle is positively related to pronuclear score after ICSI.
Shen Y, Stalf T, Mehnert C, De Santis L, Cino I, Tinneberg HR, Eichenlaub-Ritter U., Reprod. Biomed. Online 12(6), 2006
PMID: 16792851
A reliable, noninvasive technique for spindle imaging and enucleation of mammalian oocytes.
Liu L, Oldenbourg R, Trimarchi JR, Keefe DL., Nat. Biotechnol. 18(2), 2000
PMID: 10657133
The first polar body does not predict accurately the location of the metaphase II meiotic spindle in mammalian oocytes.
Silva CP, Kommineni K, Oldenbourg R, Keefe DL., Fertil. Steril. 71(4), 1999
PMID: 10202885
2-methoxyestradiol induces spindle aberrations, chromosome congression failure, and nondisjunction in mouse oocytes.
Eichenlaub-Ritter U, Winterscheidt U, Vogt E, Shen Y, Tinneberg HR, Sorensen R., Biol. Reprod. 76(5), 2007
PMID: 17229934
Arsenite induces aberrations in meiosis that can be prevented by coadministration of N-acetylcysteine in mice.
Navarro PA, Liu L, Ferriani RA, Keefe DL., Fertil. Steril. 85 Suppl 1(), 2006
PMID: 16616091
The telomere theory of reproductive senescence in women.
Keefe DL, Marquard K, Liu L., Curr. Opin. Obstet. Gynecol. 18(3), 2006
PMID: 16735827
The spindle observation and its relationship with fertilization after intracytoplasmic sperm injection in living human oocytes.
Wang WH, Meng L, Hackett RJ, Odenbourg R, Keefe DL., Fertil. Steril. 75(2), 2001
PMID: 11172838
Polscope analysis of meiotic spindle changes in living metaphase II human oocytes during the freezing and thawing procedures.
Rienzi L, Martinez F, Ubaldi F, Minasi MG, Iacobelli M, Tesarik J, Greco E., Hum. Reprod. 19(3), 2004
PMID: 14998966
Relationship between meiotic spindle location with regard to the polar body position and oocyte developmental potential after ICSI.
Rienzi L, Ubaldi F, Martinez F, Iacobelli M, Minasi MG, Ferrero S, Tesarik J, Greco E., Hum. Reprod. 18(6), 2003
PMID: 12773461
Confocal microscopic analysis of the spindle and chromosome configurations of human oocytes matured in vitro.
Li Y, Feng HL, Cao YJ, Zheng GJ, Yang Y, Mullen S, Critser JK, Chen ZJ., Fertil. Steril. 85(4), 2006
PMID: 16580356

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