Generation of chromosomal DNA during alkaline lysis and removal by reverse micellar extraction

Tschapalda K, Streitner N, Voß C, Flaschel E (2009)
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY 84(1): 199-204.

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Abstract
The separation of structurally related impurities from pharmaceutical plasmid DNA by highly scalable purification techniques is a challenge for biochemical engineering. Next to RNA, proteins, and lipopolysaccharides, the chromosomal DNA of the plasmid replicating host has to be removed. Here, we describe the application of reverse micellar extraction for the separation of chromosomal from plasmid DNA. By applying different procedures for alkaline lysis, bacterial lysates with different amounts of chromosomal DNA were generated. A reverse micellar extraction step enabled us to deplete the concentration of this impurity below the required level of 50 mg g(-1) of plasmid DNA with almost complete plasmid recovery.
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Tschapalda K, Streitner N, Voß C, Flaschel E. Generation of chromosomal DNA during alkaline lysis and removal by reverse micellar extraction. APPLIED MICROBIOLOGY AND BIOTECHNOLOGY. 2009;84(1):199-204.
Tschapalda, K., Streitner, N., Voß, C., & Flaschel, E. (2009). Generation of chromosomal DNA during alkaline lysis and removal by reverse micellar extraction. APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, 84(1), 199-204.
Tschapalda, K., Streitner, N., Voß, C., and Flaschel, E. (2009). Generation of chromosomal DNA during alkaline lysis and removal by reverse micellar extraction. APPLIED MICROBIOLOGY AND BIOTECHNOLOGY 84, 199-204.
Tschapalda, K., et al., 2009. Generation of chromosomal DNA during alkaline lysis and removal by reverse micellar extraction. APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, 84(1), p 199-204.
K. Tschapalda, et al., “Generation of chromosomal DNA during alkaline lysis and removal by reverse micellar extraction”, APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, vol. 84, 2009, pp. 199-204.
Tschapalda, K., Streitner, N., Voß, C., Flaschel, E.: Generation of chromosomal DNA during alkaline lysis and removal by reverse micellar extraction. APPLIED MICROBIOLOGY AND BIOTECHNOLOGY. 84, 199-204 (2009).
Tschapalda, Kirsten, Streitner, Nadine, Voß, Carsten, and Flaschel, Erwin. “Generation of chromosomal DNA during alkaline lysis and removal by reverse micellar extraction”. APPLIED MICROBIOLOGY AND BIOTECHNOLOGY 84.1 (2009): 199-204.
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Data provided by Europe PubMed Central.

Comparison of alkaline lysis with electroextraction and optimization of electric pulses to extract plasmid DNA from Escherichia coli.
Haberl S, Jarc M, Strancar A, Peterka M, Hodzic D, Miklavcic D., J. Membr. Biol. 246(11), 2013
PMID: 23831957

20 References

Data provided by Europe PubMed Central.

A rapid alkaline extraction procedure for screening recombinant plasmid DNA.
Birnboim HC, Doly J., Nucleic Acids Res. 7(6), 1979
PMID: 388356
Mechanical disruption of Escherichia coli for plasmid recovery.
Carlson A, Signs M, Liermann L, Boor R, Jem KJ., Biotechnol. Bioeng. 48(4), 1995
PMID: 18623491
The impact of fluid-dynamic-generated stresses on chDNA and pDNA stability during alkaline cell lysis for gene therapy products.
Chamsart S, Patel H, Hanak JA, Hitchcock AG, Nienow AW., Biotechnol. Bioeng. 75(4), 2001
PMID: 11668438

M, J Chem Eng Jpn 32(), 1999

TA, 1989
Reverse micellar extraction for downstream processing of proteins/enzymes.
Krishna SH, Srinivas ND, Raghavarao KS, Karanth NG., Adv. Biochem. Eng. Biotechnol. 75(), 2002
PMID: 11787493
Supercoiled plasmid DNA: selective purification by thiophilic/aromatic adsorption.
Lemmens R, Olsson U, Nyhammar T, Stadler J., J. Chromatogr. B Analyt. Technol. Biomed. Life Sci. 784(2), 2003
PMID: 12505777
Biochemical engineering approaches to the challenges of producing pure plasmid DNA.
Levy MS, O'Kennedy RD, Ayazi-Shamlou P, Dunnill P., Trends Biotechnol. 18(7), 2000
PMID: 10856925
Degradation of supercoiled plasmid DNA within a capillary device.
Meacle FJ, Zhang H, Papantoniou I, Ward JM, Titchener-Hooker NJ, Hoare M., Biotechnol. Bioeng. 97(5), 2007
PMID: 17115451
Proposal for a better integration of bacterial lysis into the production of plasmid DNA at large scale.
O'Mahony K, Freitag R, Hilbrig F, Muller P, Schumacher I., J. Biotechnol. 119(2), 2005
PMID: 15993505
Integration of bacteria capture via filtration and in situ lysis for recovery of plasmid DNA under industry-compatible conditions.
O'Mahony K, Freitag R, Hilbrig F, Schumacher I, Muller P., Biotechnol. Prog. 23(4), 2007
PMID: 17628077

J, 1989
Reverse micellar extraction systems for the purification of pharmaceutical grade plasmid DNA.
Streitner N, Voss C, Flaschel E., J. Biotechnol. 131(2), 2007
PMID: 17673324

N, Chem Ing Tech 80(), 2008

C, Biotechnol Annu Rev 13C(), 2007

C, Chem Eng Technol 31(), 2008

C, 2005
Production of supercoiled multimeric plasmid DNA for biopharmaceutical application.
Voss C, Schmidt T, Schleef M, Friehs K, Flaschel E., J. Biotechnol. 105(3), 2003
PMID: 14580792
Prediction of shear damage of plasmid DNA in pump and centrifuge operations using an ultra scale-down device.
Zhang H, Kong S, Booth A, Boushaba R, Levy MS, Hoare M., Biotechnol. Prog. 23(4), 2007
PMID: 17672520

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