Three-spin correlations in double electron-electron resonance

Jeschke G, Sajid M, Schulte M, Godt A (2009)

Es wurde kein Volltext hochgeladen. Nur Publikationsnachweis!
Zeitschriftenaufsatz | Veröffentlicht | Englisch
; ; ;
Abstract / Bemerkung
Distance measurements by pulse electron paramagnetic resonance techniques are increasingly applied to systems that contain more than two spin labels. Under typical experimental conditions this leads to signal contributions from sum and difference combinations of dipolar frequencies. As these contributions are neglected in the expressions used in data analysis they cause artifacts in distance distributions. It is shown that the artifacts lead to substantial broadening of the distance distribution. By measuring double electron-electron resonance (DEER) data with variable inversion efficiency of the pump pulse and fitting the data at each point in time by appropriate polynomials the pair contribution can be separated from the three-spin contribution. Experiments on a conformationally invariant triradical with equilateral triangle geometry and the corresponding biradical with the same interspin distance demonstrate that this approach leads to a considerable improvement in the distance distribution. The three-spin contribution is shown to provide information on angles between spin-spin vectors. Data for a series of triradicals with equilateral, isosceles, and scalene triangle geometry are analysed by fitting a general triangle model simultaneously to the dipolar spectra of the pair and three-spin contribution. The agreement with the expected side lengths of the triangles is satisfying.


Jeschke G, Sajid M, Schulte M, Godt A. Three-spin correlations in double electron-electron resonance. PHYSICAL CHEMISTRY CHEMICAL PHYSICS. 2009;11(31):6580-6591.
Jeschke, G., Sajid, M., Schulte, M., & Godt, A. (2009). Three-spin correlations in double electron-electron resonance. PHYSICAL CHEMISTRY CHEMICAL PHYSICS, 11(31), 6580-6591. doi:10.1039/b905724b
Jeschke, G., Sajid, M., Schulte, M., and Godt, A. (2009). Three-spin correlations in double electron-electron resonance. PHYSICAL CHEMISTRY CHEMICAL PHYSICS 11, 6580-6591.
Jeschke, G., et al., 2009. Three-spin correlations in double electron-electron resonance. PHYSICAL CHEMISTRY CHEMICAL PHYSICS, 11(31), p 6580-6591.
G. Jeschke, et al., “Three-spin correlations in double electron-electron resonance”, PHYSICAL CHEMISTRY CHEMICAL PHYSICS, vol. 11, 2009, pp. 6580-6591.
Jeschke, G., Sajid, M., Schulte, M., Godt, A.: Three-spin correlations in double electron-electron resonance. PHYSICAL CHEMISTRY CHEMICAL PHYSICS. 11, 6580-6591 (2009).
Jeschke, Gunnar, Sajid, Muhammad, Schulte, Miriam, and Godt, Adelheid. “Three-spin correlations in double electron-electron resonance”. PHYSICAL CHEMISTRY CHEMICAL PHYSICS 11.31 (2009): 6580-6591.

43 Zitationen in Europe PMC

Daten bereitgestellt von Europe PubMed Central.

An algorithm to analyze PELDOR data of rigid spin label pairs.
Marko A, Prisner TF., Phys Chem Chem Phys 15(2), 2013
PMID: 23183777
Conformational ensemble of the sodium-coupled aspartate transporter.
Georgieva ER, Borbat PP, Ginter C, Freed JH, Boudker O., Nat Struct Mol Biol 20(2), 2013
PMID: 23334289
Conformational heterogeneity of the aspartate transporter Glt(Ph).
Hänelt I, Wunnicke D, Bordignon E, Steinhoff HJ, Slotboom DJ., Nat Struct Mol Biol 20(2), 2013
PMID: 23334291
Rigid core and flexible terminus: structure of solubilized light-harvesting chlorophyll a/b complex (LHCII) measured by EPR.
Dockter C, Müller AH, Dietz C, Volkov A, Polyhach Y, Jeschke G, Paulsen H., J Biol Chem 287(4), 2012
PMID: 22147706
DEER distance measurements on proteins.
Jeschke G., Annu Rev Phys Chem 63(), 2012
PMID: 22404592
Asymmetry in the homodimeric ABC transporter MsbA recognized by a DARPin.
Mittal A, Böhm S, Grütter MG, Bordignon E, Seeger MA., J Biol Chem 287(24), 2012
PMID: 22523072
High sensitivity and versatility of the DEER experiment on nitroxide radical pairs at Q-band frequencies.
Polyhach Y, Bordignon E, Tschaggelar R, Gandra S, Godt A, Jeschke G., Phys Chem Chem Phys 14(30), 2012
PMID: 22751953
Conformational state of the MscS mechanosensitive channel in solution revealed by pulsed electron-electron double resonance (PELDOR) spectroscopy.
Pliotas C, Ward R, Branigan E, Rasmussen A, Hagelueken G, Huang H, Black SS, Booth IR, Schiemann O, Naismith JH., Proc Natl Acad Sci U S A 109(40), 2012
PMID: 23012406
A short note on the analysis of distance measurements by electron paramagnetic resonance.
Domingo Köhler S, Spitzbarth M, Diederichs K, Exner TE, Drescher M., J Magn Reson 208(1), 2011
PMID: 21044853
Pulsed dipolar spectroscopy distance measurements in biomacromolecules labeled with Gd(III) markers.
Song Y, Meade TJ, Astashkin AV, Klein EL, Enemark JH, Raitsimring A., J Magn Reson 210(1), 2011
PMID: 21388847
DEER in biological multispin-systems: a case study on the fatty acid binding to human serum albumin.
Junk MJ, Spiess HW, Hinderberger D., J Magn Reson 210(2), 2011
PMID: 21450500
W-Band pulse EPR distance measurements in peptides using Gd(3+)-dipicolinic acid derivatives as spin labels.
Gordon-Grossman M, Kaminker I, Gofman Y, Shai Y, Goldfarb D., Phys Chem Chem Phys 13(22), 2011
PMID: 21552622
Mechanism of multivalent carbohydrate-protein interactions studied by EPR spectroscopy.
Braun P, Nägele B, Wittmann V, Drescher M., Angew Chem Int Ed Engl 50(36), 2011
PMID: 21739541
A triple spin-labeling strategy coupled with DEER analysis to detect DNA modifications and enzymatic repair.
Flaender M, Sicoli G, Aci-Seche S, Reignier T, Maurel V, Saint-Pierre C, Boulard Y, Gambarelli S, Gasparutto D., Chembiochem 12(17), 2011
PMID: 21984076
Conformationally unambiguous spin labeling for distance measurements.
Sajid M, Jeschke G, Wiebcke M, Godt A., Chemistry 15(47), 2009
PMID: 19847831

34 References

Daten bereitgestellt von Europe PubMed Central.

Distance Measurements in Solid-State NMR and EPR Spectroscopy
Jeschke, 2006
How flexible are poly(para-phenyleneethynylene)s?
Godt A, Schulte M, Zimmermann H, Jeschke G., Angew. Chem. Int. Ed. Engl. 45(45), 2006
PMID: 17051574
Counting the monomers in nanometer-sized oligomers by pulsed electron-electron double resonance.
Bode BE, Margraf D, Plackmeyer J, Durner G, Prisner TF, Schiemann O., J. Am. Chem. Soc. 129(21), 2007
PMID: 17487970
Long-range distance determinations in biomacromolecules by EPR spectroscopy.
Schiemann O, Prisner TF., Q. Rev. Biophys. 40(1), 2007
PMID: 17565764
Distance measurements in the borderline region of applicability of CW EPR and DEER: a model study on a homologous series of spin-labelled peptides.
Banham JE, Baker CM, Ceola S, Day IJ, Grant GH, Groenen EJ, Rodgers CT, Jeschke G, Timmel CR., J. Magn. Reson. 191(2), 2008
PMID: 18280189
PELDOR Spectroscopy Distance Fingerprinting of the Octameric Outer-Membrane Protein Wza from Escherichia coli.
Hagelueken, Angewandte Chemie International Edition 48(16), 2009
Relative Orientation of Rigid Nitroxides by PELDOR: Beyond Distance Measurements in Nucleic Acids
Schiemann, Angewandte Chemie International Edition 48(18), 2009


Markieren/ Markierung löschen
Markierte Publikationen

Open Data PUB

Web of Science

Dieser Datensatz im Web of Science®


PMID: 19639133
PubMed | Europe PMC

Suchen in

Google Scholar