The Metabolome of Chlamydomonas reinhardtii following Induction of Anaerobic H-2 Production by Sulfur Depletion

Matthew T, Zhou W, Rupprecht J, Lim L, Thomas-Hall SR, Doebbe A, Kruse O, Hankamer B, Marx UC, Smith SM, Schenk PM (2009)
JOURNAL OF BIOLOGICAL CHEMISTRY 284(35): 23415-23425.

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Abstract
The metabolome of the model species Chlamydomonas reinhardtii has been analyzed during 120 h of sulfur depletion to induce anaerobic hydrogen (H-2) production, using NMR spectroscopy, gas chromatography coupled to mass spectrometry, and TLC. The results indicate that these unicellular green algae consume freshly supplied acetate in the medium to accumulate energy reserves during the first 24 h of sulfur depletion. In addition to the previously reported accumulation of starch, large amounts of triacylglycerides were deposited in the cells. During the early 24- to 72-h time period fermentative energy metabolism lowered the pH, H-2 was produced, and amino acid levels generally increased. In the final phase from 72 to 120 h, metabolism slowed down leading to a stabilization of pH, even though some starch and most triacylglycerides remained. We conclude that H-2 production does not slow down due to depletion of energy reserves but rather due to loss of essential functions resulting from sulfur depletion or due to a build-up of the toxic fermentative products formate and ethanol.
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Matthew T, Zhou W, Rupprecht J, et al. The Metabolome of Chlamydomonas reinhardtii following Induction of Anaerobic H-2 Production by Sulfur Depletion. JOURNAL OF BIOLOGICAL CHEMISTRY. 2009;284(35):23415-23425.
Matthew, T., Zhou, W., Rupprecht, J., Lim, L., Thomas-Hall, S. R., Doebbe, A., Kruse, O., et al. (2009). The Metabolome of Chlamydomonas reinhardtii following Induction of Anaerobic H-2 Production by Sulfur Depletion. JOURNAL OF BIOLOGICAL CHEMISTRY, 284(35), 23415-23425.
Matthew, T., Zhou, W., Rupprecht, J., Lim, L., Thomas-Hall, S. R., Doebbe, A., Kruse, O., Hankamer, B., Marx, U. C., Smith, S. M., et al. (2009). The Metabolome of Chlamydomonas reinhardtii following Induction of Anaerobic H-2 Production by Sulfur Depletion. JOURNAL OF BIOLOGICAL CHEMISTRY 284, 23415-23425.
Matthew, T., et al., 2009. The Metabolome of Chlamydomonas reinhardtii following Induction of Anaerobic H-2 Production by Sulfur Depletion. JOURNAL OF BIOLOGICAL CHEMISTRY, 284(35), p 23415-23425.
T. Matthew, et al., “The Metabolome of Chlamydomonas reinhardtii following Induction of Anaerobic H-2 Production by Sulfur Depletion”, JOURNAL OF BIOLOGICAL CHEMISTRY, vol. 284, 2009, pp. 23415-23425.
Matthew, T., Zhou, W., Rupprecht, J., Lim, L., Thomas-Hall, S.R., Doebbe, A., Kruse, O., Hankamer, B., Marx, U.C., Smith, S.M., Schenk, P.M.: The Metabolome of Chlamydomonas reinhardtii following Induction of Anaerobic H-2 Production by Sulfur Depletion. JOURNAL OF BIOLOGICAL CHEMISTRY. 284, 23415-23425 (2009).
Matthew, Timmins, Zhou, Wenxu, Rupprecht, Jens, Lim, Lysha, Thomas-Hall, Skye R., Doebbe, Anja, Kruse, Olaf, Hankamer, Ben, Marx, Ute C., Smith, Steven M., and Schenk, Peer M. “The Metabolome of Chlamydomonas reinhardtii following Induction of Anaerobic H-2 Production by Sulfur Depletion”. JOURNAL OF BIOLOGICAL CHEMISTRY 284.35 (2009): 23415-23425.
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Lopez Garcia de Lomana A, Schauble S, Valenzuela J, Imam S, Carter W, Bilgin DD, Yohn CB, Turkarslan S, Reiss DJ, Orellana MV, Price ND, Baliga NS., Biotechnol Biofuels 8(), 2015
PMID: 26633994
Lipidomic Analysis of Chlamydomonas reinhardtii under Nitrogen and Sulfur Deprivation.
Yang D, Song D, Kind T, Ma Y, Hoefkens J, Fiehn O., PLoS ONE 10(9), 2015
PMID: 26375463
Multiple regulatory mechanisms in the chloroplast of green algae: relation to hydrogen production.
Antal TK, Krendeleva TE, Tyystjarvi E., Photosyn. Res. 125(3), 2015
PMID: 25986411
Relevance of nutrient media composition for hydrogen production in Chlamydomonas.
Gonzalez-Ballester D, Jurado-Oller JL, Fernandez E., Photosyn. Res. 125(3), 2015
PMID: 25952745
Analysis of green algal growth via dynamic model simulation and process optimization.
Zhang D, Chanona EA, Vassiliadis VS, Tamburic B., Biotechnol. Bioeng. 112(10), 2015
PMID: 25855209
Microalgal Metabolic Network Model Refinement through High-Throughput Functional Metabolic Profiling.
Chaiboonchoe A, Dohai BS, Cai H, Nelson DR, Jijakli K, Salehi-Ashtiani K., Front Bioeng Biotechnol 2(), 2014
PMID: 25540776
Effects of long chain fatty acid synthesis and associated gene expression in microalga Tetraselmis sp.
Adarme-Vega TC, Thomas-Hall SR, Lim DK, Schenk PM., Mar Drugs 12(6), 2014
PMID: 24901700
Distinct mechanisms regulating gene expression coexist within the fermentative pathways in Chlamydomonas reinhardtii.
Swirsky Whitney LA, Novi G, Perata P, Loreti E., ScientificWorldJournal 2012(), 2012
PMID: 22792045

28 References

Data provided by Europe PubMed Central.

Hydrogen photoproduction is attenuated by disruption of an isoamylase gene in Chlamydomonas reinhardtii.
Posewitz MC, Smolinski SL, Kanakagiri S, Melis A, Seibert M, Ghirardi ML., Plant Cell 16(8), 2004
PMID: 15269330
Improved photobiological H2 production in engineered green algal cells.
Kruse O, Rupprecht J, Bader KP, Thomas-Hall S, Schenk PM, Finazzi G, Hankamer B., J. Biol. Chem. 280(40), 2005
PMID: 16100118

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