Screening for conditions of enhanced production of a recombinant beta-glucanase secreted into the medium by Escherichia coli

Spexard M, Beshay U, Risse JM, Miksch G, Flaschel E (2010)
BIOTECHNOLOGY LETTERS 32(2): 243-248.

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The extracellular production of a hybrid bacterial beta-glucanase using Escherichia coli was studied by using combinations of promoters of varying strength for both a beta-glucanase as the target protein and the Kil protein as the releasing factor. Four strains with different combinations of promoter strengths were cultivated in shake-flasks on four different media to assess the cross-influence of promoter and medium in a general manner. Promoters were taken from natural as well as synthetic sequences known to exhibit either weak or strong promoter strength. By far the highest extracellular glucanase activity (> 200 U ml(-1)) was achieved when a strain harbouring the kil gene under control of a strong synthetic stationary-phase promoter and the glucanase gene under control of a strong synthetic constitutive promoter was cultivated on a complex medium mainly composed of casein peptone, yeast extract, and glycerol.
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Spexard M, Beshay U, Risse JM, Miksch G, Flaschel E. Screening for conditions of enhanced production of a recombinant beta-glucanase secreted into the medium by Escherichia coli. BIOTECHNOLOGY LETTERS. 2010;32(2):243-248.
Spexard, M., Beshay, U., Risse, J. M., Miksch, G., & Flaschel, E. (2010). Screening for conditions of enhanced production of a recombinant beta-glucanase secreted into the medium by Escherichia coli. BIOTECHNOLOGY LETTERS, 32(2), 243-248.
Spexard, M., Beshay, U., Risse, J. M., Miksch, G., and Flaschel, E. (2010). Screening for conditions of enhanced production of a recombinant beta-glucanase secreted into the medium by Escherichia coli. BIOTECHNOLOGY LETTERS 32, 243-248.
Spexard, M., et al., 2010. Screening for conditions of enhanced production of a recombinant beta-glucanase secreted into the medium by Escherichia coli. BIOTECHNOLOGY LETTERS, 32(2), p 243-248.
M. Spexard, et al., “Screening for conditions of enhanced production of a recombinant beta-glucanase secreted into the medium by Escherichia coli”, BIOTECHNOLOGY LETTERS, vol. 32, 2010, pp. 243-248.
Spexard, M., Beshay, U., Risse, J.M., Miksch, G., Flaschel, E.: Screening for conditions of enhanced production of a recombinant beta-glucanase secreted into the medium by Escherichia coli. BIOTECHNOLOGY LETTERS. 32, 243-248 (2010).
Spexard, Meike, Beshay, Usama, Risse, Joe Max, Miksch, Gerd, and Flaschel, Erwin. “Screening for conditions of enhanced production of a recombinant beta-glucanase secreted into the medium by Escherichia coli”. BIOTECHNOLOGY LETTERS 32.2 (2010): 243-248.
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