Functional genomics of pH homeostasis in Corynebacterium glutamicum revealed novel links between pH response, oxidative stress, iron homeostasis and methionine synthesis

Follmann M, Ochrombel I, Kraemer R, Troetschel C, Poetsch A, Rückert C, Hueser A, Persicke M, Seiferling D, Kalinowski J, Marin K (2009)
BMC Genomics 10(1).

Download
OA
Journal Article | Published | English
Author
; ; ; ; ; ; ; ; ; ;
Abstract
Background: The maintenance of internal pH in bacterial cells is challenged by natural stress conditions, during host infection or in biotechnological production processes. Comprehensive transcriptomic and proteomic analyses has been conducted in several bacterial model systems, yet questions remain as to the mechanisms of pH homeostasis. Results: Here we present the comprehensive analysis of pH homeostasis in C. glutamicum, a bacterium of industrial importance. At pH values between 6 and 9 effective maintenance of the internal pH at 7.5 +/- 0.5 pH units was found. By DNA microarray analyses differential mRNA patterns were identified. The expression profiles were validated and extended by 1D-LC-ESI-MS/MS based quantification of soluble and membrane proteins. Regulators involved were identified and thereby participation of numerous signaling modules in pH response was found. The functional analysis revealed for the first time the occurrence of oxidative stress in C. glutamicum cells at neutral and low pH conditions accompanied by activation of the iron starvation response. Intracellular metabolite pool analysis unraveled inhibition of the TCA and other pathways at low pH. Methionine and cysteine synthesis were found to be activated via the McbR regulator, cysteine accumulation was observed and addition of cysteine was shown to be toxic under acidic conditions. Conclusions: Novel limitations for C. glutamicum at non-optimal pH values were identified by a comprehensive analysis on the level of the transcriptome, proteome, and metabolome indicating a functional link between pH acclimatization, oxidative stress, iron homeostasis, and metabolic alterations. The results offer new insights into bacterial stress physiology and new starting points for bacterial strain design or pathogen defense.
Publishing Year
ISSN
PUB-ID

Cite this

Follmann M, Ochrombel I, Kraemer R, et al. Functional genomics of pH homeostasis in Corynebacterium glutamicum revealed novel links between pH response, oxidative stress, iron homeostasis and methionine synthesis. BMC Genomics. 2009;10(1).
Follmann, M., Ochrombel, I., Kraemer, R., Troetschel, C., Poetsch, A., Rückert, C., Hueser, A., et al. (2009). Functional genomics of pH homeostasis in Corynebacterium glutamicum revealed novel links between pH response, oxidative stress, iron homeostasis and methionine synthesis. BMC Genomics, 10(1).
Follmann, M., Ochrombel, I., Kraemer, R., Troetschel, C., Poetsch, A., Rückert, C., Hueser, A., Persicke, M., Seiferling, D., Kalinowski, J., et al. (2009). Functional genomics of pH homeostasis in Corynebacterium glutamicum revealed novel links between pH response, oxidative stress, iron homeostasis and methionine synthesis. BMC Genomics 10.
Follmann, M., et al., 2009. Functional genomics of pH homeostasis in Corynebacterium glutamicum revealed novel links between pH response, oxidative stress, iron homeostasis and methionine synthesis. BMC Genomics, 10(1).
M. Follmann, et al., “Functional genomics of pH homeostasis in Corynebacterium glutamicum revealed novel links between pH response, oxidative stress, iron homeostasis and methionine synthesis”, BMC Genomics, vol. 10, 2009.
Follmann, M., Ochrombel, I., Kraemer, R., Troetschel, C., Poetsch, A., Rückert, C., Hueser, A., Persicke, M., Seiferling, D., Kalinowski, J., Marin, K.: Functional genomics of pH homeostasis in Corynebacterium glutamicum revealed novel links between pH response, oxidative stress, iron homeostasis and methionine synthesis. BMC Genomics. 10, (2009).
Follmann, Martin, Ochrombel, Ines, Kraemer, Reinhard, Troetschel, Christian, Poetsch, Ansgar, Rückert, Christian, Hueser, Andrea, Persicke, Marcus, Seiferling, Dominic, Kalinowski, Jörn, and Marin, Kay. “Functional genomics of pH homeostasis in Corynebacterium glutamicum revealed novel links between pH response, oxidative stress, iron homeostasis and methionine synthesis”. BMC Genomics 10.1 (2009).
Main File(s)
File Name
Access Level
OA Open Access

This data publication is cited in the following publications:
This publication cites the following data publications:

17 Citations in Europe PMC

Data provided by Europe PubMed Central.

Anaerobic growth of Corynebacterium glutamicum via mixed-acid fermentation.
Michel A, Koch-Koerfges A, Krumbach K, Brocker M, Bott M., Appl. Environ. Microbiol. 81(21), 2015
PMID: 26276118
Metabolic engineering of Corynebacterium glutamicum strain ATCC13032 to produce L-methionine.
Qin T, Hu X, Hu J, Wang X., Biotechnol. Appl. Biochem. 62(4), 2015
PMID: 25196586
Assessment of robustness against dissolved oxygen/substrate oscillations for C. glutamicum DM1933 in two-compartment bioreactor.
Kaß F, Hariskos I, Michel A, Brandt HJ, Spann R, Junne S, Wiechert W, Neubauer P, Oldiges M., Bioprocess Biosyst Eng 37(6), 2014
PMID: 24218302
Conversion of Corynebacterium glutamicum from an aerobic respiring to an aerobic fermenting bacterium by inactivation of the respiratory chain.
Koch-Koerfges A, Pfelzer N, Platzen L, Oldiges M, Bott M., Biochim. Biophys. Acta 1827(6), 2013
PMID: 23416842
Characterization of shikimate dehydrogenase homologues of Corynebacterium glutamicum.
Kubota T, Tanaka Y, Hiraga K, Inui M, Yukawa H., Appl. Microbiol. Biotechnol. 97(18), 2013
PMID: 23306642
Proteomics of corynebacteria: From biotechnology workhorses to pathogens.
Poetsch A, Haussmann U, Burkovski A., Proteomics 11(15), 2011
PMID: 21674800
Identification of the membrane protein SucE and its role in succinate transport in Corynebacterium glutamicum.
Huhn S, Jolkver E, Kramer R, Marin K., Appl. Microbiol. Biotechnol. 89(2), 2011
PMID: 20809072
Corynebacterium glutamicum exhibits a membrane-related response to a small ferrocene-conjugated antimicrobial peptide.
Franzel B, Frese C, Penkova M, Metzler-Nolte N, Bandow JE, Wolters DA., J. Biol. Inorg. Chem. 15(8), 2010
PMID: 20658302
L-Glutamine as a nitrogen source for Corynebacterium glutamicum: derepression of the AmtR regulon and implications for nitrogen sensing.
Rehm N, Georgi T, Hiery E, Degner U, Schmiedl A, Burkovski A, Bott M., Microbiology (Reading, Engl.) 156(Pt 10), 2010
PMID: 20656783

56 References

Data provided by Europe PubMed Central.

EMMA: a platform for consistent storage and efficient analysis of microarray data.
Dondrup M, Goesmann A, Bartels D, Kalinowski J, Krause L, Linke B, Rupp O, Sczyrba A, Puhler A, Meyer F., J. Biotechnol. 106(2-3), 2003
PMID: 14651856
A detergent- and cyanogen bromide-free method for integral membrane proteomics: application to Halobacterium purple membranes and the human epidermal membrane proteome.
Blonder J, Conrads TP, Yu LR, Terunuma A, Janini GM, Issaq HJ, Vogel JC, Veenstra TD., Proteomics 4(1), 2004
PMID: 14730670
Detecting differential and correlated protein expression in label-free shotgun proteomics.
Zhang B, VerBerkmoes NC, Langston MA, Uberbacher E, Hettich RL, Samatova NF., J. Proteome Res. 5(11), 2006
PMID: 17081042
Technical advance: simultaneous analysis of metabolites in potato tuber by gas chromatography-mass spectrometry.
Roessner U, Wagner C, Kopka J, Trethewey RN, Willmitzer L., Plant J. 23(1), 2000
PMID: 10929108

Export

0 Marked Publications

Open Data PUB

Web of Science

View record in Web of Science®

Sources

PMID: 20025733
PubMed | Europe PMC

Search this title in

Google Scholar