Sequential Expression of Bacterial Virulence and Plant Defense Genes During Infection of Tomato with Clavibacter michiganensis subsp michiganensis

Chalupowicz L, Cohen-Kandli M, Dror O, Eichenlaub R, Gartemann K-H, Sessa G, Barash I, Manulis-Sasson S (2010)
PHYTOPATHOLOGY 100(3): 252-261.

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Abstract
The molecular interactions between Clavibacter michiganensis subsp. michiganensis and tomato plant were studied by following the expression of bacterial virulence and host-defense genes during early stages of infection. The C. michiganensis subsp. michiganensis genes included the plasmid-borne cellulase (celA) and the serine protease (pat-1), and the serine proteases chpC and ppaA, residing on the chp/tomA pathogenicity island (PAI). Gene expression was measured following tomato inoculation with Cmm382 (wild type), Cmm100 (lacking the plasmids pCM1 and pCM2), and Cmm27 (lacking the PAI). Transcriptional analysis revealed that celA and pat-1 were significantly induced in Cmm382 at initial 12 to 72 h, whereas chpC and ppaA were highly expressed only 96 h after inoculation. Interdependence between the expression of chromosomal and of plasmid-located genes was revealed: expression of celA and pat-1 was substantially reduced in the absence of the chp/tomA PAI, whereas chpC and ppaA expressions were reduced in the absence of the virulence plasmids. Transcription of chromosomal genes involved in cell wall degradation (i.e., pelA1, celB, xysA, and xysB), was also induced at early stages of infection. Expression of the host-defense genes, chitinase class II and pathogenesis-related protein-5 isoform was induced in the absence of the PAI at early stages of infection, suggesting that PAI-located genes are involved in suppression of tomato basal defenses.
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Chalupowicz L, Cohen-Kandli M, Dror O, et al. Sequential Expression of Bacterial Virulence and Plant Defense Genes During Infection of Tomato with Clavibacter michiganensis subsp michiganensis. PHYTOPATHOLOGY. 2010;100(3):252-261.
Chalupowicz, L., Cohen-Kandli, M., Dror, O., Eichenlaub, R., Gartemann, K. - H., Sessa, G., Barash, I., et al. (2010). Sequential Expression of Bacterial Virulence and Plant Defense Genes During Infection of Tomato with Clavibacter michiganensis subsp michiganensis. PHYTOPATHOLOGY, 100(3), 252-261.
Chalupowicz, L., Cohen-Kandli, M., Dror, O., Eichenlaub, R., Gartemann, K. - H., Sessa, G., Barash, I., and Manulis-Sasson, S. (2010). Sequential Expression of Bacterial Virulence and Plant Defense Genes During Infection of Tomato with Clavibacter michiganensis subsp michiganensis. PHYTOPATHOLOGY 100, 252-261.
Chalupowicz, L., et al., 2010. Sequential Expression of Bacterial Virulence and Plant Defense Genes During Infection of Tomato with Clavibacter michiganensis subsp michiganensis. PHYTOPATHOLOGY, 100(3), p 252-261.
L. Chalupowicz, et al., “Sequential Expression of Bacterial Virulence and Plant Defense Genes During Infection of Tomato with Clavibacter michiganensis subsp michiganensis”, PHYTOPATHOLOGY, vol. 100, 2010, pp. 252-261.
Chalupowicz, L., Cohen-Kandli, M., Dror, O., Eichenlaub, R., Gartemann, K.-H., Sessa, G., Barash, I., Manulis-Sasson, S.: Sequential Expression of Bacterial Virulence and Plant Defense Genes During Infection of Tomato with Clavibacter michiganensis subsp michiganensis. PHYTOPATHOLOGY. 100, 252-261 (2010).
Chalupowicz, L., Cohen-Kandli, M., Dror, O., Eichenlaub, Rudolf, Gartemann, Karl-Heinz, Sessa, G., Barash, I., and Manulis-Sasson, S. “Sequential Expression of Bacterial Virulence and Plant Defense Genes During Infection of Tomato with Clavibacter michiganensis subsp michiganensis”. PHYTOPATHOLOGY 100.3 (2010): 252-261.
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4 Citations in Europe PMC

Data provided by Europe PubMed Central.

Studies on the colonization of axenically grown tomato plants by a GFP-tagged strain of Clavibacter michiganensis subsp. michiganensis
Lelis FMV, Czajkowski R, de Souza RM, Ribeiro DH, van der Wolf JM., Eur. J. Plant Pathol. 139(1), 2014
PMID: IND500743147
Colonization and movement of GFP-labeled Clavibacter michiganensis subsp. michiganensis during tomato infection.
Chalupowicz L, Zellermann EM, Fluegel M, Dror O, Eichenlaub R, Gartemann KH, Savidor A, Sessa G, Iraki N, Barash I, Manulis-Sasson S., Phytopathology 102(1), 2012
PMID: 21879791

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