An intracellular pH gradient in the anammox bacterium Kuenenia stuttgartiensis as evaluated by P-31 NMR

van der Star WRL, Dijkema C, de Waard P, Picioreanu C, Strous M, van Loosdrecht MCM (2010)
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY 86(1): 311-317.

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Abstract
The cytoplasm of anaerobic ammonium oxidizing (anammox) bacteria consists of three compartments separated by membranes. It has been suggested that a proton motive force may be generated over the membrane of the innermost compartment, the "anammoxosome". P-31 nuclear magnetic resonance (NMR) spectroscopy was employed to investigate intracellular pH differences in the anammox bacterium Kuenenia stuttgartiensis. With in vivo NMR, spectra were recorded of active, highly concentrated suspensions of K. stuttgartiensis in a wide-bore NMR tube. At different external pH values, two stable and distinct phosphate peaks were apparent in the recorded spectra. These peaks were equivalent with pH values of 7.3 and 6.3 and suggested the presence of a proton motive force over an intracytoplasmic membrane in K. stuttgartiensis. This study provides for the second time-after discovery of acidocalcisome-like compartments in Agrobacterium tumefaciens-evidence for an intracytoplasmic pH gradient in a chemotrophic prokaryotic cell.
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van der Star WRL, Dijkema C, de Waard P, Picioreanu C, Strous M, van Loosdrecht MCM. An intracellular pH gradient in the anammox bacterium Kuenenia stuttgartiensis as evaluated by P-31 NMR. APPLIED MICROBIOLOGY AND BIOTECHNOLOGY. 2010;86(1):311-317.
van der Star, W. R. L., Dijkema, C., de Waard, P., Picioreanu, C., Strous, M., & van Loosdrecht, M. C. M. (2010). An intracellular pH gradient in the anammox bacterium Kuenenia stuttgartiensis as evaluated by P-31 NMR. APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, 86(1), 311-317.
van der Star, W. R. L., Dijkema, C., de Waard, P., Picioreanu, C., Strous, M., and van Loosdrecht, M. C. M. (2010). An intracellular pH gradient in the anammox bacterium Kuenenia stuttgartiensis as evaluated by P-31 NMR. APPLIED MICROBIOLOGY AND BIOTECHNOLOGY 86, 311-317.
van der Star, W.R.L., et al., 2010. An intracellular pH gradient in the anammox bacterium Kuenenia stuttgartiensis as evaluated by P-31 NMR. APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, 86(1), p 311-317.
W.R.L. van der Star, et al., “An intracellular pH gradient in the anammox bacterium Kuenenia stuttgartiensis as evaluated by P-31 NMR”, APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, vol. 86, 2010, pp. 311-317.
van der Star, W.R.L., Dijkema, C., de Waard, P., Picioreanu, C., Strous, M., van Loosdrecht, M.C.M.: An intracellular pH gradient in the anammox bacterium Kuenenia stuttgartiensis as evaluated by P-31 NMR. APPLIED MICROBIOLOGY AND BIOTECHNOLOGY. 86, 311-317 (2010).
van der Star, Wouter R. L., Dijkema, Cor, de Waard, Pieter, Picioreanu, Cristian, Strous, Marc, and van Loosdrecht, Mark C. M. “An intracellular pH gradient in the anammox bacterium Kuenenia stuttgartiensis as evaluated by P-31 NMR”. APPLIED MICROBIOLOGY AND BIOTECHNOLOGY 86.1 (2010): 311-317.
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31 References

Data provided by Europe PubMed Central.

Autotrophic growth of anaerobic ammonium-oxidizing microorganisms in a fluidized bed reactor
Graaf AA, Bruijn P, Robertson LA, Jetten MSM, Kuenen JG., 1996
Metabolic pathway of anaerobic ammonium oxidation on the basis of N studies in a fluidized bed reactor
Graaf AA, Bruijn P, Robertson LA, Jetten MSM, Kuenen JG., 1997
Startup of reactors for anoxic ammonium oxidation: experiences from the first full-scale anammox reactor in Rotterdam.
van der Star WR, Abma WR, Blommers D, Mulder JW, Tokutomi T, Strous M, Picioreanu C, van Loosdrecht MC., Water Res. 41(18), 2007
PMID: 17583763
The membrane bioreactor: a novel tool to grow anammox bacteria as free cells.
van der Star WR, Miclea AI, van Dongen UG, Muyzer G, Picioreanu C, van Loosdrecht MC., Biotechnol. Bioeng. 101(2), 2008
PMID: 18421799
The anammoxosome: an intracytoplasmic compartment in anammox bacteria.
van Niftrik LA, Fuerst JA, Sinninghe Damste JS, Kuenen JG, Jetten MS, Strous M., FEMS Microbiol. Lett. 233(1), 2004
PMID: 15098544
Linking ultrastructure and function in four genera of anaerobic ammonium-oxidizing bacteria: cell plan, glycogen storage, and localization of cytochrome C proteins.
van Niftrik L, Geerts WJ, van Donselaar EG, Humbel BM, Webb RI, Fuerst JA, Verkleij AJ, Jetten MS, Strous M., J. Bacteriol. 190(2), 2008
PMID: 17993524

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