The LacI/GalR family transcriptional regulator UriR negatively controls uridine utilization of Corynebacterium glutamicum by binding to catabolite-responsive element (cre)-like sequences

Brinkrolf K, Ploeger S, Solle S, Brune I, Nentwich SS, Hueser AT, Kalinowski J, Pühler A, Tauch A (2008)
MICROBIOLOGY 154(4): 1068-1081.

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Zeitschriftenaufsatz | Veröffentlicht | Englisch
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Abstract / Bemerkung
The Cg1547 protein of Corynebacterium glutamicum ATCC 13032 is a member of the LacI/GalR family of DNA-binding transcriptional regulators. A defined deletion in the cg1547 gene, now designated uriR (uridine utilization regulator), resulted in the mutant strain C. glutamicum KB1547. Comparison of gene expression levels in C. glutamicum KB1547 and the wild-type strain revealed enhanced expression of the uriR operon genes cg1546 (ribokinase), cg1545 (uridine transporter) and cg1543 (uridine-pref erring nucleoside hydrolase). Gene expression of the uriR operon was stimulated by the presence of either uridine or ribose. Growth assays with C. glutamicum mutants showed that functional Cg 1543 and Cg 1545 proteins are essential for the utilization of uridine as the sole carbon source. Transcriptional regulation of the uriR operon is mediated by a 29 bp palindromic sequence composed of two catabolite-responsive element (cre)-like sequences and located in between the mapped -10 promoter region and the start codon of uriR. A similar cre sequence was detected in the upstream region of rbsK2 (cg2554), coding for a second ribokinase in C. glutamicum ATCC 13032. DNA band-shift assays with a streptavidin-tagged UriR protein and labelled oligonucleotides including the cre-like sequences of uriR and rbsK2 demonstrated the specific binding of the purified regulator in vitro. Whole-genome DNA microarray hybridizations comparing the gene expression in C. glutamicum KB1547 with that of the wild-type strain revealed that UriR is a pathway-specific repressor of genes involved in uridine utilization in C. glutamicum.
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Zeitschriftentitel
MICROBIOLOGY
Band
154
Zeitschriftennummer
4
Seite
1068-1081
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Brinkrolf K, Ploeger S, Solle S, et al. The LacI/GalR family transcriptional regulator UriR negatively controls uridine utilization of Corynebacterium glutamicum by binding to catabolite-responsive element (cre)-like sequences. MICROBIOLOGY. 2008;154(4):1068-1081.
Brinkrolf, K., Ploeger, S., Solle, S., Brune, I., Nentwich, S. S., Hueser, A. T., Kalinowski, J., et al. (2008). The LacI/GalR family transcriptional regulator UriR negatively controls uridine utilization of Corynebacterium glutamicum by binding to catabolite-responsive element (cre)-like sequences. MICROBIOLOGY, 154(4), 1068-1081. doi:10.1099/mic.0.2007/014001-0
Brinkrolf, K., Ploeger, S., Solle, S., Brune, I., Nentwich, S. S., Hueser, A. T., Kalinowski, J., Pühler, A., and Tauch, A. (2008). The LacI/GalR family transcriptional regulator UriR negatively controls uridine utilization of Corynebacterium glutamicum by binding to catabolite-responsive element (cre)-like sequences. MICROBIOLOGY 154, 1068-1081.
Brinkrolf, K., et al., 2008. The LacI/GalR family transcriptional regulator UriR negatively controls uridine utilization of Corynebacterium glutamicum by binding to catabolite-responsive element (cre)-like sequences. MICROBIOLOGY, 154(4), p 1068-1081.
K. Brinkrolf, et al., “The LacI/GalR family transcriptional regulator UriR negatively controls uridine utilization of Corynebacterium glutamicum by binding to catabolite-responsive element (cre)-like sequences”, MICROBIOLOGY, vol. 154, 2008, pp. 1068-1081.
Brinkrolf, K., Ploeger, S., Solle, S., Brune, I., Nentwich, S.S., Hueser, A.T., Kalinowski, J., Pühler, A., Tauch, A.: The LacI/GalR family transcriptional regulator UriR negatively controls uridine utilization of Corynebacterium glutamicum by binding to catabolite-responsive element (cre)-like sequences. MICROBIOLOGY. 154, 1068-1081 (2008).
Brinkrolf, Karina, Ploeger, Svenja, Solle, Sandra, Brune, Iris, Nentwich, Svenia S., Hueser, Andrea T., Kalinowski, Jörn, Pühler, Alfred, and Tauch, Andreas. “The LacI/GalR family transcriptional regulator UriR negatively controls uridine utilization of Corynebacterium glutamicum by binding to catabolite-responsive element (cre)-like sequences”. MICROBIOLOGY 154.4 (2008): 1068-1081.

13 Zitationen in Europe PMC

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PMID: 22094976
Complete genome sequence, lifestyle, and multi-drug resistance of the human pathogen Corynebacterium resistens DSM 45100 isolated from blood samples of a leukemia patient.
Schröder J, Maus I, Meyer K, Wördemann S, Blom J, Jaenicke S, Schneider J, Trost E, Tauch A., BMC Genomics 13(), 2012
PMID: 22524407
Global transcriptome response in Lactobacillus sakei during growth on ribose.
McLeod A, Snipen L, Naterstad K, Axelsson L., BMC Microbiol 11(), 2011
PMID: 21702908
Two nucleoside transporters in Lactococcus lactis with different substrate specificities.
Martinussen J, Sørensen C, Jendresen CB, Kilstrup M., Microbiology 156(pt 10), 2010
PMID: 20595258
SUPERFAMILY--sophisticated comparative genomics, data mining, visualization and phylogeny.
Wilson D, Pethica R, Zhou Y, Talbot C, Vogel C, Madera M, Chothia C, Gough J., Nucleic Acids Res 37(database issue), 2009
PMID: 19036790
Characterization of the LacI-type transcriptional repressor RbsR controlling ribose transport in Corynebacterium glutamicum ATCC 13032.
Nentwich SS, Brinkrolf K, Gaigalat L, Hüser AT, Rey DA, Mohrbach T, Marin K, Pühler A, Tauch A, Kalinowski J., Microbiology 155(pt 1), 2009
PMID: 19118356
EMMA 2--a MAGE-compliant system for the collaborative analysis and integration of microarray data.
Dondrup M, Albaum SP, Griebel T, Henckel K, Jünemann S, Kahlke T, Kleindt CK, Küster H, Linke B, Mertens D, Mittard-Runte V, Neuweger H, Runte KJ, Tauch A, Tille F, Pühler A, Goesmann A., BMC Bioinformatics 10(), 2009
PMID: 19200358

53 References

Daten bereitgestellt von Europe PubMed Central.

Structural analysis of lac repressor bound to allosteric effectors.
Daber R, Stayrook S, Rosenberg A, Lewis M., J. Mol. Biol. 370(4), 2007
PMID: 17543986
Expression of Corynebacterium glutamicum glycolytic genes varies with carbon source and growth phase.
Han SO, Inui M, Yukawa H., Microbiology (Reading, Engl.) 153(Pt 7), 2007
PMID: 17600063

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