The SNF1-type serine-threonine protein kinase SAPK4 regulates stress-responsive gene expression in rice

Diedhiou CJ, Popova OV, Dietz K-J, Golldack D (2008)
BMC PLANT BIOLOGY 8(1): 49.

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BACKGROUND: Plants respond to extracellularly perceived abiotic stresses such as low temperature, drought, and salinity by activation of complex intracellular signaling cascades that regulate acclimatory biochemical and physiological changes. Protein kinases are major signal transduction factors that have a central role in mediating acclimation to environmental changes in eukaryotic organisms. In this study, we characterized the function of the sucrose nonfermenting 1-related protein kinase2 (SnRK2) SAPK4 in the salt stress response of rice. RESULTS: Translational fusion of SAPK4 with the green fluorescent protein (GFP) showed subcellular localization in cytoplasm and nucleus. To examine the role of SAPK4 in salt tolerance we generated transgenic rice plants with over-expression of rice SAPK4 under control of the CaMV-35S promoter. Induced expression of SAPK4 resulted in improved germination, growth and development under salt stress both in seedlings and mature plants. In response to salt stress, the SAPK4-overexpressing rice accumulated less Na+ and Cl- and showed improved photosynthesis. SAPK4-regulated genes with functions in ion homeostasis and oxidative stress response were identified: the vacuolar H+-ATPase, the Na+/H+ antiporter NHX1, the Cl- channel OsCLC1 and a catalase. CONCLUSION: Our results show that SAPK4 regulates ion homeostasis and growth and development under salinity and suggest function of SAPK4 as a regulatory factor in plant salt stress acclimation. Identification of signaling elements involved in stress adaptation in plants presents a powerful approach to identify transcriptional activators of adaptive mechanisms to environmental changes that have the potential to improve tolerance in crop plants.
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BMC PLANT BIOLOGY
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8
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1
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49
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Diedhiou CJ, Popova OV, Dietz K-J, Golldack D. The SNF1-type serine-threonine protein kinase SAPK4 regulates stress-responsive gene expression in rice. BMC PLANT BIOLOGY. 2008;8(1):49.
Diedhiou, C. J., Popova, O. V., Dietz, K. - J., & Golldack, D. (2008). The SNF1-type serine-threonine protein kinase SAPK4 regulates stress-responsive gene expression in rice. BMC PLANT BIOLOGY, 8(1), 49. doi:10.1186/1471-2229-8-49
Diedhiou, C. J., Popova, O. V., Dietz, K. - J., and Golldack, D. (2008). The SNF1-type serine-threonine protein kinase SAPK4 regulates stress-responsive gene expression in rice. BMC PLANT BIOLOGY 8, 49.
Diedhiou, C.J., et al., 2008. The SNF1-type serine-threonine protein kinase SAPK4 regulates stress-responsive gene expression in rice. BMC PLANT BIOLOGY, 8(1), p 49.
C.J. Diedhiou, et al., “The SNF1-type serine-threonine protein kinase SAPK4 regulates stress-responsive gene expression in rice”, BMC PLANT BIOLOGY, vol. 8, 2008, pp. 49.
Diedhiou, C.J., Popova, O.V., Dietz, K.-J., Golldack, D.: The SNF1-type serine-threonine protein kinase SAPK4 regulates stress-responsive gene expression in rice. BMC PLANT BIOLOGY. 8, 49 (2008).
Diedhiou, Calliste J., Popova, Olga V., Dietz, Karl-Josef, and Golldack, Dortje. “The SNF1-type serine-threonine protein kinase SAPK4 regulates stress-responsive gene expression in rice”. BMC PLANT BIOLOGY 8.1 (2008): 49.
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PMID: 24465084
Cloning and characterization of TaSnRK2.3, a novel SnRK2 gene in common wheat.
Tian S, Mao X, Zhang H, Chen S, Zhai C, Yang S, Jing R., J Exp Bot 64(7), 2013
PMID: 23630328
Bioengineering for salinity tolerance in plants: state of the art.
Agarwal PK, Shukla PS, Gupta K, Jha B., Mol Biotechnol 54(1), 2013
PMID: 22539206
A suite of new genes defining salinity stress tolerance in seedlings of contrasting rice genotypes.
Soda N, Kushwaha HR, Soni P, Singla-Pareek SL, Pareek A., Funct Integr Genomics 13(3), 2013
PMID: 23813016
Insights into genomics of salt stress response in rice.
Kumar K, Kumar M, Kim SR, Ryu H, Cho YG., Rice (N Y) 6(1), 2013
PMID: 24280112
Phosphatidic acid, a versatile water-stress signal in roots.
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PMID: 24391659
Comparative proteomic analysis of early salt stress-responsive proteins in roots of SnRK2 transgenic rice.
Nam MH, Huh SM, Kim KM, Park WJ, Seo JB, Cho K, Kim DY, Kim BG, Yoon IS., Proteome Sci 10(), 2012
PMID: 22462395
Identification of salt treated proteins in sorghum using gene ontology linkage.
Sekhwal MK, Swami AK, Sarin R, Sharma V., Physiol Mol Biol Plants 18(3), 2012
PMID: 23814435
Osmotic stress signaling via protein kinases.
Fujii H, Zhu JK., Cell Mol Life Sci 69(19), 2012
PMID: 22828864
Water stress induces up-regulation of DOF1 and MIF1 transcription factors and down-regulation of proteins involved in secondary metabolism in amaranth roots (Amaranthus hypochondriacus L.).
Huerta-Ocampo JA, León-Galván MF, Ortega-Cruz LB, Barrera-Pacheco A, De León-Rodríguez A, Mendoza-Hernández G, de la Rosa AP., Plant Biol (Stuttg) 13(3), 2011
PMID: 21489098
SNF1-related protein kinases 2 are negatively regulated by a plant-specific calcium sensor.
Bucholc M, Ciesielski A, Goch G, Anielska-Mazur A, Kulik A, Krzywińska E, Dobrowolska G., J Biol Chem 286(5), 2011
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Cyclin dependent protein kinases and stress responses in plants.
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PMID: 21512322
Cloning and characterization of a maize SnRK2 protein kinase gene confers enhanced salt tolerance in transgenic Arabidopsis.
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SnRK2 protein kinases--key regulators of plant response to abiotic stresses.
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PMID: 22136638

57 References

Daten bereitgestellt von Europe PubMed Central.

Oxidative stress, antioxidants and stress tolerance.
Mittler R., Trends Plant Sci. 7(9), 2002
PMID: 12234732
Hydrogen peroxide signalling.
Neill S, Desikan R, Hancock J., Curr. Opin. Plant Biol. 5(5), 2002
PMID: 12183176
Catalase is a sink for H2O2 and is indispensable for stress defence in C3 plants.
Willekens H, Chamnongpol S, Davey M, Schraudner M, Langebartels C, Van Montagu M, Inze D, Van Camp W., EMBO J. 16(16), 1997
PMID: 9305623
Modular cloning in plant cells.
Karimi M, De Meyer B, Hilson P., Trends Plant Sci. 10(3), 2005
PMID: 15749466
Subcellular distribution of the V-ATPase complex in plant cells, and in vivo localisation of the 100 kDa subunit VHA-a within the complex.
Kluge C, Seidel T, Bolte S, Sharma SS, Hanitzsch M, Satiat-Jeunemaitre B, Ross J, Sauer M, Golldack D, Dietz KJ., BMC Cell Biol. 5(), 2004
PMID: 15310389

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