Transcript profiling indicates that the absence of PsbO affects the coordination of C and N metabolism in Synechocystis sp PCC 6803

Schriek S, Aguirre-von-Wobeser E, Nodop A, Becker A, Ibelings BW, Bok J, Staiger D, Matthijs HCP, Pistorius EK, Michel K-P (2008)
Physiologia Plantarum 133(3): 525-543.

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Abstract
Transcript profiling of nitrate-grown Synechocystis sp. PCC 6803 PsbO-free mutant cells in comparison to wild-type (WT) detected substantial deviations. Because we had previously observed phenotypical differences between Synechocystis sp. PCC 6803 WT and its corresponding PsbO-free mutant when cultivated with L-arginine as sole N source and a light intensity of 200 mu mol photons m(-2) s(-1), we also performed transcript profiling for both strains grown either with nitrate or with L-arginine as sole N source. We observed a total number of 520 differentially regulated transcripts in Synechocystis WT because of a shift from nitrate- to L-arginine-containing BG11 medium, while we detected only 13 differentially regulated transcripts for the PsbO-free mutant. Thus, the PsbO-free Synechocystis mutant had already undergone a preconditioning process for growth with L-arginine in comparison to WT. While Synechocystis WT suffered from growth with L-arginine at a light intensity of 200 mu mol photons m(-2) s(-1), the PsbO-free mutant developed only a minor stress phenotype. In summary, our results suggest that the absence of PsbO in Synechocystis affects the coordination of photosynthesis/respiration and L-arginine metabolism through complex probably redox-mediated regulatory pathways. In addition, we show that a comparison of the transcriptomes of nitrate-grown Synechococcus elongatus PCC 7942 WT cells and its corresponding PsbO-free mutant cells resulted in only a few differentially regulated transcripts between both strains. The absence of the manganese/calcium-stabilizing PsbO protein of PSII with an assigned regulatory function for photosynthetic water oxidation causes bigger changes in the transcriptome of the permissive photoheterotrophically growing Synechocystis sp. PCC 6803 than in the transcriptome of the obligate photoautotrophically growing S. elongatus PCC 7942.
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Schriek S, Aguirre-von-Wobeser E, Nodop A, et al. Transcript profiling indicates that the absence of PsbO affects the coordination of C and N metabolism in Synechocystis sp PCC 6803. Physiologia Plantarum. 2008;133(3):525-543.
Schriek, S., Aguirre-von-Wobeser, E., Nodop, A., Becker, A., Ibelings, B. W., Bok, J., Staiger, D., et al. (2008). Transcript profiling indicates that the absence of PsbO affects the coordination of C and N metabolism in Synechocystis sp PCC 6803. Physiologia Plantarum, 133(3), 525-543.
Schriek, S., Aguirre-von-Wobeser, E., Nodop, A., Becker, A., Ibelings, B. W., Bok, J., Staiger, D., Matthijs, H. C. P., Pistorius, E. K., and Michel, K. - P. (2008). Transcript profiling indicates that the absence of PsbO affects the coordination of C and N metabolism in Synechocystis sp PCC 6803. Physiologia Plantarum 133, 525-543.
Schriek, S., et al., 2008. Transcript profiling indicates that the absence of PsbO affects the coordination of C and N metabolism in Synechocystis sp PCC 6803. Physiologia Plantarum, 133(3), p 525-543.
S. Schriek, et al., “Transcript profiling indicates that the absence of PsbO affects the coordination of C and N metabolism in Synechocystis sp PCC 6803”, Physiologia Plantarum, vol. 133, 2008, pp. 525-543.
Schriek, S., Aguirre-von-Wobeser, E., Nodop, A., Becker, A., Ibelings, B.W., Bok, J., Staiger, D., Matthijs, H.C.P., Pistorius, E.K., Michel, K.-P.: Transcript profiling indicates that the absence of PsbO affects the coordination of C and N metabolism in Synechocystis sp PCC 6803. Physiologia Plantarum. 133, 525-543 (2008).
Schriek, Sarah, Aguirre-von-Wobeser, Eneas, Nodop, Anke, Becker, Anke, Ibelings, Bas W., Bok, Jasper, Staiger, Dorothee, Matthijs, Hans C. P., Pistorius, Elfriede K., and Michel, Klaus-Peter. “Transcript profiling indicates that the absence of PsbO affects the coordination of C and N metabolism in Synechocystis sp PCC 6803”. Physiologia Plantarum 133.3 (2008): 525-543.
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Detection of an L-amino acid dehydrogenase activity in Synechocystis sp. PCC 6803.
Schriek S, Kahmann U, Staiger D, Pistorius EK, Michel KP., J. Exp. Bot. 60(3), 2009
PMID: 19213808

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Minorsky, PLANT PHYSIOLOGY 147(1), 2008

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