Analysis of outer membrane vesicle associated proteins isolated from the plant pathogenic bacterium Xanthomonas campestris pv. campestris

Sidhu VK, Vorhölter F-J, Niehaus K, Watt SA (2008)
BMC Microbiology 8(1).

Download
OA
Journal Article | Published | English
Author
; ; ;
Abstract
Background: Outer membrane vesicles (OMVs) are released from the outer membrane of many Gram-negative bacteria. These extracellular compartments are known to transport compounds involved in cell-cell signalling as well as virulence associated proteins, e. g. the cytolysine from enterotoxic E. coli. Results: We have demonstrated that Xanthomonas campestris pv. campestris (Xcc) releases OMVs into the culture supernatant during growth. A proteome study identified 31 different proteins that associate with the OMV fraction of which half are virulence-associated. A comparison with the most abundant outer membrane ( OM) proteins revealed that some proteins are enriched in the OMV fraction. This may be connected to differences in the LPS composition between the OMVs and the OM. Furthermore, a comparison of the OMV proteomes from two different culture media indicated that the culture conditions have an impact on the protein composition. Interestingly, the proteins that are common to both culture conditions are mainly involved in virulence. Conclusion: Outer membrane vesicles released from the OM of Xcc contain membrane- and virulence-associated proteins. Future experiments will prove whether these structures can serve as "vehicles" for the transport of virulence factors into the host membrane.
Publishing Year
ISSN
PUB-ID

Cite this

Sidhu VK, Vorhölter F-J, Niehaus K, Watt SA. Analysis of outer membrane vesicle associated proteins isolated from the plant pathogenic bacterium Xanthomonas campestris pv. campestris. BMC Microbiology. 2008;8(1).
Sidhu, V. K., Vorhölter, F. - J., Niehaus, K., & Watt, S. A. (2008). Analysis of outer membrane vesicle associated proteins isolated from the plant pathogenic bacterium Xanthomonas campestris pv. campestris. BMC Microbiology, 8(1).
Sidhu, V. K., Vorhölter, F. - J., Niehaus, K., and Watt, S. A. (2008). Analysis of outer membrane vesicle associated proteins isolated from the plant pathogenic bacterium Xanthomonas campestris pv. campestris. BMC Microbiology 8.
Sidhu, V.K., et al., 2008. Analysis of outer membrane vesicle associated proteins isolated from the plant pathogenic bacterium Xanthomonas campestris pv. campestris. BMC Microbiology, 8(1).
V.K. Sidhu, et al., “Analysis of outer membrane vesicle associated proteins isolated from the plant pathogenic bacterium Xanthomonas campestris pv. campestris”, BMC Microbiology, vol. 8, 2008.
Sidhu, V.K., Vorhölter, F.-J., Niehaus, K., Watt, S.A.: Analysis of outer membrane vesicle associated proteins isolated from the plant pathogenic bacterium Xanthomonas campestris pv. campestris. BMC Microbiology. 8, (2008).
Sidhu, Vishaldeep K., Vorhölter, Frank-Jörg, Niehaus, Karsten, and Watt, Steven A. “Analysis of outer membrane vesicle associated proteins isolated from the plant pathogenic bacterium Xanthomonas campestris pv. campestris”. BMC Microbiology 8.1 (2008).
Main File(s)
File Name
Access Level
OA Open Access
Last Uploaded
2015-12-22T10:31:39Z

This data publication is cited in the following publications:
This publication cites the following data publications:

29 Citations in Europe PMC

Data provided by Europe PubMed Central.

Xanthomonas campestris pv. vesicatoria Secretes Proteases and Xylanases via the Xps Type II Secretion System and Outer Membrane Vesicles.
Sole M, Scheibner F, Hoffmeister AK, Hartmann N, Hause G, Rother A, Jordan M, Lautier M, Arlat M, Buttner D., J. Bacteriol. 197(17), 2015
PMID: 26124239
Molecular characterization of outer membrane vesicles released from Acinetobacter radioresistens and their potential roles in pathogenesis.
Fulsundar S, Kulkarni HM, Jagannadham MV, Nair R, Keerthi S, Sant P, Pardesi K, Bellare J, Chopade BA., Microb. Pathog. 83-84(), 2015
PMID: 25940676
Antibody-mediated immunity induced by engineered Escherichia coli OMVs carrying heterologous antigens in their lumen.
Fantappie L, de Santis M, Chiarot E, Carboni F, Bensi G, Jousson O, Margarit I, Grandi G., J Extracell Vesicles 3(), 2014
PMID: 25147647

46 References

Data provided by Europe PubMed Central.

Potential role of molecular mimicry between Helicobacter pylori lipopolysaccharide and host Lewis blood group antigens in autoimmunity.
Appelmelk BJ, Simoons-Smit I, Negrini R, Moran AP, Aspinall GO, Forte JG, De Vries T, Quan H, Verboom T, Maaskant JJ, Ghiara P, Kuipers EJ, Bloemena E, Tadema TM, Townsend RR, Tyagarajan K, Crothers JM Jr, Monteiro MA, Savio A, De Graaff J., Infect. Immun. 64(6), 1996
PMID: 8675304
Molecular signals required for type III secretion and translocation of the Xanthomonas campestris AvrBs2 protein to pepper plants.
Mudgett MB, Chesnokova O, Dahlbeck D, Clark ET, Rossier O, Bonas U, Staskawicz BJ., Proc. Natl. Acad. Sci. U.S.A. 97(24), 2000
PMID: 11078519
Direct biochemical evidence for type III secretion-dependent translocation of the AvrBs2 effector protein into plant cells.
Casper-Lindley C, Dahlbeck D, Clark ET, Staskawicz BJ., Proc. Natl. Acad. Sci. U.S.A. 99(12), 2002
PMID: 12060777
A hidden Markov model for predicting transmembrane helices in protein sequences
Glasgow J, Sonnhammer EL, von G, Krogh A., 1998
The release of outer membrane vesicles from the strains of enterotoxigenic Escherichia coli.
Wai SN, Takade A, Amako K., Microbiol. Immunol. 39(7), 1995
PMID: 8569529
Identification of prokaryotic and eukaryotic signal peptides and prediction of their cleavage sites.
Nielsen H, Engelbrecht J, Brunak S, von Heijne G., Protein Eng. 10(1), 1997
PMID: 9051728
Adhesion of cells to surfaces coated with polylysine. Applications to electron microscopy.
Mazia D, Schatten G, Sale W., J. Cell Biol. 66(1), 1975
PMID: 1095595
Proteome analysis of matrix vesicles isolated from femurs of chicken embryo.
Balcerzak M, Malinowska A, Thouverey C, Sekrecka A, Dadlez M, Buchet R, Pikula S., Proteomics 8(1), 2008
PMID: 18095356
Proteomic identification of endothelial proteins isolated in situ from atherosclerotic aorta via systemic perfusion.
Wu J, Liu W, Sousa E, Qiu Y, Pittman DD, Maganti V, Feldman J, Gill D, Lu Z, Dorner AJ, Schaub R, Tan XY., J. Proteome Res. 6(12), 2007
PMID: 17997517
WM Keck Foundation Biotechnology ResourceLaboratory
AUTHOR UNKNOWN, 0
Swiss-Prot Protein knowledgebase
AUTHOR UNKNOWN, 0

Export

0 Marked Publications

Open Data PUB

Web of Science

View record in Web of Science®

Sources

PMID: 18518965
PubMed | Europe PMC

Search this title in

Google Scholar