T-DNA-mediated transfer of Agrobacterium tumefaciens chromosomal DNA into plants

Uelker B, Li Y, Rosso MG, Logemann E, Somssich IE, Weisshaar B (2008)
Nature Biotechnology 26(9): 1015-1017.

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Besides the well-documented integration of DNA flanked by the transfer DNA borders, occasional insertion of fragments from the tumor-inducing plasmid into plant genomes has also been reported during Agrobacterium tumefaciens-mediated transformation. We demonstrate that large (up to approximately 18 kb) gene-bearing fragments of Agrobacterium chromosomal DNA (AchrDNA) can be integrated into Arabidopsis thaliana genomic DNA during transformation. One in every 250 transgenic plants may carry AchrDNA fragments. This has implications for horizontal gene transfer and indicates a need for greater scrutiny of transgenic plants for undesired bacterial DNA.
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Uelker B, Li Y, Rosso MG, Logemann E, Somssich IE, Weisshaar B. T-DNA-mediated transfer of Agrobacterium tumefaciens chromosomal DNA into plants. Nature Biotechnology. 2008;26(9):1015-1017.
Uelker, B., Li, Y., Rosso, M. G., Logemann, E., Somssich, I. E., & Weisshaar, B. (2008). T-DNA-mediated transfer of Agrobacterium tumefaciens chromosomal DNA into plants. Nature Biotechnology, 26(9), 1015-1017.
Uelker, B., Li, Y., Rosso, M. G., Logemann, E., Somssich, I. E., and Weisshaar, B. (2008). T-DNA-mediated transfer of Agrobacterium tumefaciens chromosomal DNA into plants. Nature Biotechnology 26, 1015-1017.
Uelker, B., et al., 2008. T-DNA-mediated transfer of Agrobacterium tumefaciens chromosomal DNA into plants. Nature Biotechnology, 26(9), p 1015-1017.
B. Uelker, et al., “T-DNA-mediated transfer of Agrobacterium tumefaciens chromosomal DNA into plants”, Nature Biotechnology, vol. 26, 2008, pp. 1015-1017.
Uelker, B., Li, Y., Rosso, M.G., Logemann, E., Somssich, I.E., Weisshaar, B.: T-DNA-mediated transfer of Agrobacterium tumefaciens chromosomal DNA into plants. Nature Biotechnology. 26, 1015-1017 (2008).
Uelker, Bekir, Li, Yong, Rosso, Mario G., Logemann, Elke, Somssich, Imre E., and Weisshaar, Bernd. “T-DNA-mediated transfer of Agrobacterium tumefaciens chromosomal DNA into plants”. Nature Biotechnology 26.9 (2008): 1015-1017.
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17 Citations in Europe PMC

Data provided by Europe PubMed Central.

Agrobacterium-mediated transient MaFT expression in mulberry (Morus alba L.) leaves.
Wu SL, Yang XB, Liu LQ, Jiang T, Wu H, Su C, Qian YH, Jiao F., Biosci. Biotechnol. Biochem. 79(8), 2015
PMID: 26024368
Whole-genome analysis of herbicide-tolerant mutant rice generated by Agrobacterium-mediated gene targeting.
Endo M, Kumagai M, Motoyama R, Sasaki-Yamagata H, Mori-Hosokawa S, Hamada M, Kanamori H, Nagamura Y, Katayose Y, Itoh T, Toki S., Plant Cell Physiol. 56(1), 2015
PMID: 25378689
Sri Lankan cassava mosaic virus replication associated protein (Rep) triggers transposition of IS426 in Agrobacterium.
Resmi TR, Nivedhitha S, Karthikeyan C, Veluthambi K., FEMS Microbiol. Lett. 360(1), 2014
PMID: 25135797
Physical methods for genetic transformation of fungi and yeast.
Rivera AL, Magana-Ortiz D, Gomez-Lim M, Fernandez F, Loske AM., Phys Life Rev 11(2), 2014
PMID: 24507729
Genome engineering of Agrobacterium tumefaciens using the lambda Red recombination system.
Hu S, Fu J, Huang F, Ding X, Stewart AF, Xia L, Zhang Y., Appl. Microbiol. Biotechnol. 98(5), 2014
PMID: 24297480
A whole-genome analysis of a transgenic rice seed-based edible vaccine against cedar pollen allergy.
Kawakatsu T, Kawahara Y, Itoh T, Takaiwa F., DNA Res. 20(6), 2013
PMID: 23956243
A novel and highly efficient method for genetic transformation of fungi employing shock waves.
Magana-Ortiz D, Coconi-Linares N, Ortiz-Vazquez E, Fernandez F, Loske AM, Gomez-Lim MA., Fungal Genet. Biol. 56(), 2013
PMID: 23583899
Genetic transformation of grapevine cells using the minimal cassette technology: the need of 3'-end protection.
Sanjurjo L, Vidal JR, Segura A, de la Torre F., J. Biotechnol. 163(4), 2013
PMID: 23219889
Physical methods for genetic plant transformation.
Rivera AL, Gomez-Lim M, Fernandez F, Loske AM., Phys Life Rev 9(3), 2012
PMID: 22704230
Cisgenic barley with improved phytase activity.
Holme IB, Dionisio G, Brinch-Pedersen H, Wendt T, Madsen CK, Vincze E, Holm PB., Plant Biotechnol. J. 10(2), 2012
PMID: 21955685
Bioinformatics and the allergy assessment of agricultural biotechnology products: industry practices and recommendations.
Ladics GS, Cressman RF, Herouet-Guicheney C, Herman RA, Privalle L, Song P, Ward JM, McClain S., Regul. Toxicol. Pharmacol. 60(1), 2011
PMID: 21320564
Identification of virulence genes in the corn pathogen Colletotrichum graminicola by Agrobacterium tumefaciens-mediated transformation.
Munch S, Ludwig N, Floss DS, Sugui JA, Koszucka AM, Voll LM, Sonnewald U, Deising HB., Mol. Plant Pathol. 12(1), 2011
PMID: 21118348
An archived activation tagged population of Arabidopsis thaliana to facilitate forward genetics approaches.
Robinson SJ, Tang LH, Mooney BA, McKay SJ, Clarke WE, Links MG, Karcz S, Regan S, Wu YY, Gruber MY, Cui D, Yu M, Parkin IA., BMC Plant Biol. 9(), 2009
PMID: 19646253
A non-canonical transferred DNA insertion at the BRI1 locus in Arabidopsis thaliana.
Zhao Z, Zhu Y, Erhardt M, Ruan Y, Shen WH., J Integr Plant Biol 51(4), 2009
PMID: 19341406
Agrobacterium-mediated DNA transfer, and then some.
Gelvin SB., Nat. Biotechnol. 26(9), 2008
PMID: 18779811

16 References

Data provided by Europe PubMed Central.

Insertion sequences.
Mahillon J, Chandler M., Microbiol. Mol. Biol. Rev. 62(3), 1998
PMID: 9729608
Agrobacterium tumefaciens transfers extremely long T-DNAs by a unidirectional mechanism.
Miranda A, Janssen G, Hodges L, Peralta EG, Ream W., J. Bacteriol. 174(7), 1992
PMID: 1551847
More T-DNA than meets the eye
SMITH, Trends in Plant Science 3(3), 1998
FLAGdb/FST: a database of mapped flanking insertion sites (FSTs) of Arabidopsis thaliana T-DNA transformants.
Samson F, Brunaud V, Balzergue S, Dubreucq B, Lepiniec L, Pelletier G, Caboche M, Lecharny A., Nucleic Acids Res. 30(1), 2002
PMID: 11752264
The genome of the natural genetic engineer Agrobacterium tumefaciens C58.
Wood DW, Setubal JC, Kaul R, Monks DE, Kitajima JP, Okura VK, Zhou Y, Chen L, Wood GE, Almeida NF Jr, Woo L, Chen Y, Paulsen IT, Eisen JA, Karp PD, Bovee D Sr, Chapman P, Clendenning J, Deatherage G, Gillet W, Grant C, Kutyavin T, Levy R, Li MJ, McClelland E, Palmieri A, Raymond C, Rouse G, Saenphimmachak C, Wu Z, Romero P, Gordon D, Zhang S, Yoo H, Tao Y, Biddle P, Jung M, Krespan W, Perry M, Gordon-Kamm B, Liao L, Kim S, Hendrick C, Zhao ZY, Dolan M, Chumley F, Tingey SV, Tomb JF, Gordon MP, Olson MV, Nester EW., Science 294(5550), 2001
PMID: 11743193
Genome sequence of the plant pathogen and biotechnology agent Agrobacterium tumefaciens C58.
Goodner B, Hinkle G, Gattung S, Miller N, Blanchard M, Qurollo B, Goldman BS, Cao Y, Askenazi M, Halling C, Mullin L, Houmiel K, Gordon J, Vaudin M, Iartchouk O, Epp A, Liu F, Wollam C, Allinger M, Doughty D, Scott C, Lappas C, Markelz B, Flanagan C, Crowell C, Gurson J, Lomo C, Sear C, Strub G, Cielo C, Slater S., Science 294(5550), 2001
PMID: 11743194
E. coli chromosomal DNA in a transgene locus created by microprojectile bombardment in tobacco.
Ulker B, Weissinger AK, Spiker S., Transgenic Res. 11(3), 2002
PMID: 12113463
A high-throughput Arabidopsis reverse genetics system.
Sessions A, Burke E, Presting G, Aux G, McElver J, Patton D, Dietrich B, Ho P, Bacwaden J, Ko C, Clarke JD, Cotton D, Bullis D, Snell J, Miguel T, Hutchison D, Kimmerly B, Mitzel T, Katagiri F, Glazebrook J, Law M, Goff SA., Plant Cell 14(12), 2002
PMID: 12468722
Genome-wide insertional mutagenesis of Arabidopsis thaliana.
Alonso JM, Stepanova AN, Leisse TJ, Kim CJ, Chen H, Shinn P, Stevenson DK, Zimmerman J, Barajas P, Cheuk R, Gadrinab C, Heller C, Jeske A, Koesema E, Meyers CC, Parker H, Prednis L, Ansari Y, Choy N, Deen H, Geralt M, Hazari N, Hom E, Karnes M, Mulholland C, Ndubaku R, Schmidt I, Guzman P, Aguilar-Henonin L, Schmid M, Weigel D, Carter DE, Marchand T, Risseeuw E, Brogden D, Zeko A, Crosby WL, Berry CC, Ecker JR., Science 301(5633), 2003
PMID: 12893945
An Arabidopsis thaliana T-DNA mutagenized population (GABI-Kat) for flanking sequence tag-based reverse genetics.
Rosso MG, Li Y, Strizhov N, Reiss B, Dekker K, Weisshaar B., Plant Mol. Biol. 53(1-2), 2003
PMID: 14756321
Mutational analysis of the conserved domains of a T-region border repeat of Agrobacterium tumefaciens.
van Haaren MJ, Sedee NJ, de Boer HA, Schilperoort RA, Hooykaas PJ., Plant Mol. Biol. 13(5), 1989
PMID: 2491670
Sequences homologous to Agrobacterium rhizogenes T-DNA in the genomes of uninfected plants
White, Nature 301(5898), 1983
Plant-derived transfer DNAs.
Rommens CM, Bougri O, Yan H, Humara JM, Owen J, Swords K, Ye J., Plant Physiol. 139(3), 2005
PMID: 16244143
A case of promiscuity: Agrobacterium's endless hunt for new partners.
Lacroix B, Tzfira T, Vainstein A, Citovsky V., Trends Genet. 22(1), 2006
PMID: 16289425


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