Insight into the plasmid metagenome of wastewater treatment plant bacteria showing reduced susceptibility to antimicrobial drugs analysed by the 454-pyrosequencing technology

Szczepanowski R, Bekel T, Goesmann A, Krause L, Kroemeke H, Kaiser O, Eichler W, Pühler A, Schlueter A (2008)
JOURNAL OF BIOTECHNOLOGY 136(1-2): 54-64.

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Zeitschriftenaufsatz | Veröffentlicht | Englisch
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Abstract / Bemerkung
Wastewater treatment plants (WWTPs) are a reservoir for bacteria harbouring antibiotic resistance plasmids. To get a comprehensive overview on the plasmid nietagenome of WWTP bacteria showing reduced susceptibility to certain antimicrobial drugs an ultrafast sequencing approach applying the 454-technology was carried out. One run on the GS 20 System yielded 346,427 reads with an average read length of 104 bases resulting in a total of 36,071,493 bases sequence data. The obtained plasmid metagenome was analysed and functionally annotated by means of the Sequence Analysis and Management System (SAMS) software package. Known plasmid genes could be identified within the WWTP plasmid metagenome data set by BLAST searches using the NCBI Plasmid Database. Most abundant hits represent genes involved in plasmid replication, stability, mobility and transposition. Mapping of plasmid metagenome reads to completely sequenced plasmids revealed that many sequences could be assigned to the cryptic pAsa plasmids previously identified in Aeromonas salmonicida subsp. salmonicida and to the accessory modules of the conjugative IncU resistance plasmid pFBAOT6 of Aeromonas punctata. Matches of sequence reads to antibiotic resistance genes indicate that plasmids from WWTP bacteria encode resistances to all major classes Of antimicrobial drugs. Plasmid metagenome sequence reads Could be assembled into 605 contigs with a minimum length of 500 bases. Contigs predominantly encode plasmid survival functions and transposition enzymes. (C) 2008 Elsevier B.V. All rights reserved.
Erscheinungsjahr
Zeitschriftentitel
JOURNAL OF BIOTECHNOLOGY
Band
136
Zeitschriftennummer
1-2
Seite
54-64
ISSN
PUB-ID

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Szczepanowski R, Bekel T, Goesmann A, et al. Insight into the plasmid metagenome of wastewater treatment plant bacteria showing reduced susceptibility to antimicrobial drugs analysed by the 454-pyrosequencing technology. JOURNAL OF BIOTECHNOLOGY. 2008;136(1-2):54-64.
Szczepanowski, R., Bekel, T., Goesmann, A., Krause, L., Kroemeke, H., Kaiser, O., Eichler, W., et al. (2008). Insight into the plasmid metagenome of wastewater treatment plant bacteria showing reduced susceptibility to antimicrobial drugs analysed by the 454-pyrosequencing technology. JOURNAL OF BIOTECHNOLOGY, 136(1-2), 54-64. doi:10.1016/j.jbiotec.2008.03.020
Szczepanowski, R., Bekel, T., Goesmann, A., Krause, L., Kroemeke, H., Kaiser, O., Eichler, W., Pühler, A., and Schlueter, A. (2008). Insight into the plasmid metagenome of wastewater treatment plant bacteria showing reduced susceptibility to antimicrobial drugs analysed by the 454-pyrosequencing technology. JOURNAL OF BIOTECHNOLOGY 136, 54-64.
Szczepanowski, R., et al., 2008. Insight into the plasmid metagenome of wastewater treatment plant bacteria showing reduced susceptibility to antimicrobial drugs analysed by the 454-pyrosequencing technology. JOURNAL OF BIOTECHNOLOGY, 136(1-2), p 54-64.
R. Szczepanowski, et al., “Insight into the plasmid metagenome of wastewater treatment plant bacteria showing reduced susceptibility to antimicrobial drugs analysed by the 454-pyrosequencing technology”, JOURNAL OF BIOTECHNOLOGY, vol. 136, 2008, pp. 54-64.
Szczepanowski, R., Bekel, T., Goesmann, A., Krause, L., Kroemeke, H., Kaiser, O., Eichler, W., Pühler, A., Schlueter, A.: Insight into the plasmid metagenome of wastewater treatment plant bacteria showing reduced susceptibility to antimicrobial drugs analysed by the 454-pyrosequencing technology. JOURNAL OF BIOTECHNOLOGY. 136, 54-64 (2008).
Szczepanowski, Rafael, Bekel, Thomas, Goesmann, Alexander, Krause, Lutz, Kroemeke, Holger, Kaiser, Olaf, Eichler, Wolfgang, Pühler, Alfred, and Schlueter, Andreas. “Insight into the plasmid metagenome of wastewater treatment plant bacteria showing reduced susceptibility to antimicrobial drugs analysed by the 454-pyrosequencing technology”. JOURNAL OF BIOTECHNOLOGY 136.1-2 (2008): 54-64.

31 Zitationen in Europe PMC

Daten bereitgestellt von Europe PubMed Central.

Mobility of plasmids.
Smillie C, Garcillán-Barcia MP, Francia MV, Rocha EP, de la Cruz F., Microbiol Mol Biol Rev 74(3), 2010
PMID: 20805406
The Sequence Analysis and Management System -- SAMS-2.0: data management and sequence analysis adapted to changing requirements from traditional sanger sequencing to ultrafast sequencing technologies.
Bekel T, Henckel K, Küster H, Meyer F, Mittard Runte V, Neuweger H, Paarmann D, Rupp O, Zakrzewski M, Pühler A, Stoye J, Goesmann A., J Biotechnol 140(1-2), 2009
PMID: 19297685
Detection of 140 clinically relevant antibiotic-resistance genes in the plasmid metagenome of wastewater treatment plant bacteria showing reduced susceptibility to selected antibiotics.
Szczepanowski R, Linke B, Krahn I, Gartemann KH, Gützkow T, Eichler W, Pühler A, Schlüter A., Microbiology 155(pt 7), 2009
PMID: 19389756
Conjugative plasmids: vessels of the communal gene pool.
Norman A, Hansen LH, Sørensen SJ., Philos Trans R Soc Lond B Biol Sci 364(1527), 2009
PMID: 19571247
Genetic diversity and composition of a plasmid metagenome from a wastewater treatment plant.
Schlüter A, Krause L, Szczepanowski R, Goesmann A, Pühler A., J Biotechnol 136(1-2), 2008
PMID: 18603322

45 References

Daten bereitgestellt von Europe PubMed Central.

IncP-1-beta plasmid pGNB1 isolated from a bacterial community from a wastewater treatment plant mediates decolorization of triphenylmethane dyes.
Schluter A, Krahn I, Kollin F, Bonemann G, Stiens M, Szczepanowski R, Schneiker S, Puhler A., Appl. Environ. Microbiol. 73(20), 2007
PMID: 17675426
Genetic diversity and composition of a plasmid metagenome from a wastewater treatment plant.
Schluter A, Krause L, Szczepanowski R, Goesmann A, Puhler A., J. Biotechnol. 136(1-2), 2008
PMID: 18603322
Novel macrolide resistance module carried by the IncP-1beta resistance plasmid pRSB111, isolated from a wastewater treatment plant.
Szczepanowski R, Krahn I, Bohn N, Puhler A, Schluter A., Antimicrob. Agents Chemother. 51(2), 2007
PMID: 17101677
The COG database: an updated version includes eukaryotes.
Tatusov RL, Fedorova ND, Jackson JD, Jacobs AR, Kiryutin B, Koonin EV, Krylov DM, Mazumder R, Mekhedov SL, Nikolskaya AN, Rao BS, Smirnov S, Sverdlov AV, Vasudevan S, Wolf YI, Yin JJ, Natale DA., BMC Bioinformatics 4(), 2003
PMID: 12969510
Occurrence of integron-associated resistance gene cassettes located on antibiotic resistance plasmids isolated from a wastewater treatment plant.
Tennstedt T, Szczepanowski R, Braun S, Puhler A, Schluter A., FEMS Microbiol. Ecol. 45(3), 2003
PMID: 19719593
Paradigms of plasmid organization.
Thomas CM., Mol. Microbiol. 37(3), 2000
PMID: 10931342
Mechanisms of, and barriers to, horizontal gene transfer between bacteria.
Thomas CM, Nielsen KM., Nat. Rev. Microbiol. 3(9), 2005
PMID: 16138099
Novel GES/IBC extended-spectrum beta-lactamase variants with carbapenemase activity in clinical enterobacteria.
Vourli S, Giakkoupi P, Miriagou V, Tzelepi E, Vatopoulos AC, Tzouvelekis LS., FEMS Microbiol. Lett. 234(2), 2004
PMID: 15135524
MvirDB--a microbial database of protein toxins, virulence factors and antibiotic resistance genes for bio-defence applications.
Zhou CE, Smith J, Lam M, Zemla A, Dyer MD, Slezak T., Nucleic Acids Res. 35(Database issue), 2007
PMID: 17090593
Mosaic structure of p1658/97, a 125-kilobase plasmid harboring an active amplicon with the extended-spectrum beta-lactamase gene blaSHV-5.
Zienkiewicz M, Kern-Zdanowicz I, Golebiewski M, Zylinska J, Mieczkowski P, Gniadkowski M, Bardowski J, Ceglowski P., Antimicrob. Agents Chemother. 51(4), 2007
PMID: 17220406

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