Factors that influence the extracellular expression of streptavidin in Escherichia coli using a bacteriocin release protein

Miksch G, Ryu S, Risse JM, Flaschel E (2008)
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY 81(2): 319-326.

Journal Article | Published | English

No fulltext has been uploaded

Abstract
Aiming to increase production of recombinant streptavidin in Escherichia coli, the effect of different leader sequences, different promoter strengths of the bacteriocin release protein (kil), host strain and medium composition on the expression and secretion into the medium was investigated. Expression vectors containing an expression or secretion unit were constructed with different combinations of leader sequence for the streptavidin gene and promoters for the kil gene and streptavidin gene. Results showed that a high-level extracellular production of streptavidin could be accomplished with E. coli BL21(DE3) by using the leader sequence of the phoA gene, a strong stationary-phase promoter for the kil gene and supplementation of the medium by glycine. Using a stationary-phase promoter for the expression of streptavidin had a negative effect.
Publishing Year
ISSN
eISSN
PUB-ID

Cite this

Miksch G, Ryu S, Risse JM, Flaschel E. Factors that influence the extracellular expression of streptavidin in Escherichia coli using a bacteriocin release protein. APPLIED MICROBIOLOGY AND BIOTECHNOLOGY. 2008;81(2):319-326.
Miksch, G., Ryu, S., Risse, J. M., & Flaschel, E. (2008). Factors that influence the extracellular expression of streptavidin in Escherichia coli using a bacteriocin release protein. APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, 81(2), 319-326.
Miksch, G., Ryu, S., Risse, J. M., and Flaschel, E. (2008). Factors that influence the extracellular expression of streptavidin in Escherichia coli using a bacteriocin release protein. APPLIED MICROBIOLOGY AND BIOTECHNOLOGY 81, 319-326.
Miksch, G., et al., 2008. Factors that influence the extracellular expression of streptavidin in Escherichia coli using a bacteriocin release protein. APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, 81(2), p 319-326.
G. Miksch, et al., “Factors that influence the extracellular expression of streptavidin in Escherichia coli using a bacteriocin release protein”, APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, vol. 81, 2008, pp. 319-326.
Miksch, G., Ryu, S., Risse, J.M., Flaschel, E.: Factors that influence the extracellular expression of streptavidin in Escherichia coli using a bacteriocin release protein. APPLIED MICROBIOLOGY AND BIOTECHNOLOGY. 81, 319-326 (2008).
Miksch, Gerd, Ryu, Stella, Risse, Joe Max, and Flaschel, Erwin. “Factors that influence the extracellular expression of streptavidin in Escherichia coli using a bacteriocin release protein”. APPLIED MICROBIOLOGY AND BIOTECHNOLOGY 81.2 (2008): 319-326.
This data publication is cited in the following publications:
This publication cites the following data publications:

3 Citations in Europe PMC

Data provided by Europe PubMed Central.

A secretory system for bacterial production of high-profile protein targets.
Kotzsch A, Vernet E, Hammarstrom M, Berthelsen J, Weigelt J, Graslund S, Sundstrom M., Protein Sci. 20(3), 2011
PMID: 21308845
Extracellular recombinant protein production from Escherichia coli.
Ni Y, Chen R., Biotechnol. Lett. 31(11), 2009
PMID: 19597765

26 References

Data provided by Europe PubMed Central.


P, Biotechnol Lett 13(), 1991

Export

0 Marked Publications

Open Data PUB

Web of Science

View record in Web of Science®

Sources

PMID: 18795286
PubMed | Europe PMC

Search this title in

Google Scholar